Novel metabolites from Penicillium crustosum, including penitrem E, a tremorgenic mycotoxin

Kyriakidis, Nikolaos B.; Waight, E. S.; Day, J. B.; Mantle, Peter G.

doi:10.1128/aem.42.1.61-62.1981

Cited by 39 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The m/z of UM does not correspond with those of the penitrems provided by Kyriakidis et al (1981) and metabolites of other Penicillium cultures provided by Smedsgaard and Frisvad (1996), Smedsgaard (1997) and Smedsgaard and Nielsen (2005). It can therefore be said that the novel mycotoxin cannot be assigned to any known metabolite reported in literature.…”

Section: Discussionmentioning

confidence: 81%

Mycotoxic nephropathy in Bulgarian pigs and chickens: complex aetiology and similarity to Balkan Endemic Nephropathy

Stoev

Dutton

Njobeh

et al. 2010

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

Spontaneous nephropathy in Bulgaria, which is observed frequently during meat inspection and which differs morphologically from the classical description of mycotoxic porcine/chicken nephropathy as made in Denmark, was found to have a multi-mycotoxic aetiology being mainly provoked by a combined effect of ochratoxin A, penicillic acid and fumonisin B1 in addition to a not-yet-known metabolite. Mean contamination levels of ochratoxin A were consecutively low (188.8 and 376.4 microg kg(-1)) in contrast to high contamination levels of fumonisin B1 (5564.1 and 3254.5 microg kg(-1)) and penicillic acid (838.6 and 904.9 microg kg(-1)) for 2006 and 2007, respectively. Some other mycotoxins with lower importance such as citrinin, penitrem A, etc., may also influence clinicopathological picture of this nephropathy. A heavy contamination with Gibberella fujikuroi var. moniliformis (Fusarium verticillioides) and Penicillium aurantiogriseum complex (mainly Penicillium polonicum) was observed in almost all examined feed samples coming from pig and chick farms with nephropathy problems from Bulgaria. In contrast, low contamination with Aspergillus ochraceus, Penicillium verrucosum and Penicillium citrinum was observed in the same feed samples and these species were isolated as very rare components of the mycobiota.

show abstract

Section: Discussionmentioning

confidence: 81%

Mycotoxic nephropathy in Bulgarian pigs and chickens: complex aetiology and similarity to Balkan Endemic Nephropathy

Stoev

Dutton

Njobeh

et al. 2010

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

show abstract

“…Penicillium crustosum (Rundberget et al, 2004) and P. polonicum are common fungal species predominantly associated with food spoilage worldwide. Their metabolic profiles (production of roquefortine C, the penitrems) have been also investigated (Kyriakidis et al, 1981). The novel metabolite presented in this study, in addition to some other mycotoxins as ochratoxin A and fumonisin B 1 (Stoev et al, 1998;, was also found in Bulgarian feed samples taken from farms with mycotoxic porcine nephropathy (unpublished results), which suggests its possible involvement in this nephropathy.…”

Section: Discussionmentioning

confidence: 53%

“…The lack of adequate evidences for the definitive relationship between mycotoxins and human disease does not necessarily imply that dietary exposure does not represent a potential risk (Stoev, 2007). In developing countries, many individuals are not only malnourished but are also chronically exposed to high levels of mycotoxins in their diet (Kyriakidis et al, 1981;Smedsgaard and Frisvad, 1996;Smedsgaard, 1997;Smedsgaard and Nielsen, 2004;Stoev, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of novel metabolite and its cytotoxic effect on human lymphocyte cells in comparison to other mycotoxins

Njobeh

Dutton²,

Chuturgoon³

et al. 2009

International Journal of Biological and Chemical Sciences

View full text Add to dashboard Cite

A novel metabolite of P. polonicum was recently isolated from Cameroonian Zea mays by means of preparative silica gel thin layer chromatographic method. The potential cytotoxic effects of this novel compound was further tested, in vitro, against cultured human mononuclear lymphocyte cells over 18-hr period in comparison to ochratoxin A and T-2 toxin. At low concentrations (0.15, 0.31 and 0.63 µg/ml), the toxicity of the novel metabolite was similar to that of T-2 toxin, but was found to be significantly (p<0.05) less toxic than ochratoxin A. The increase of concentration levels of all investigated mycotoxins from 0.15 to 5.0 µg/ml significantly (R 2 =0.8; p<0.001) decreased the cell viability. These findings indicate that the newly identified compound has a toxic effect on lymphocyte cell viability and might be a potential health risk. Having in mind the circumstance, that the novel mycotoxin is frequently found in Cameroonian food commodities as well as in animal feeds from farms with nephropathy problems in Bulgaria, further studies are required on the impact of chronic dose administration of this mycotoxin on human and animal health.

show abstract

“…Compound 43 was dereplicated as atrovenetin, a polyketide isolated from Penicillium paraherquei with antioxidant activity [61]. Adding to this list of bioactivities, the terpenoids territrem C ( 33 ) from Aspergillus terreus [62], penitrem E ( 49 ) from P. crustosum [63], and compound 53 (penitrem A or pennigritrem from Penicillium sp.) [64] are mycotoxins with tremorgenic activity in mice [63].…”

Section: Discussionmentioning

confidence: 99%

Rapid Metabolome and Bioactivity Profiling of Fungi Associated with the Leaf and Rhizosphere of the Baltic Seagrass Zostera marina

et al. 2019

View full text Add to dashboard Cite

Zostera marina (eelgrass) is a marine foundation species with key ecological roles in coastal habitats. Its bacterial microbiota has been well studied, but very little is known about its mycobiome. In this study, we have isolated and identified 13 fungal strains, dominated by Penicillium species (10 strains), from the leaf and the root rhizosphere of Baltic Z. marina. The organic extracts of the fungi that were cultured by an OSMAC (One-Strain–Many-Compounds) regime using five liquid culture media under both static and shaking conditions were investigated for their chemical and bioactivity profiles. All extracts showed strong anti-quorum sensing activity, and the majority of the Penicillium extracts displayed antimicrobial or anti-biofilm activity against Gram-negative environmental marine and human pathogens. HPLC-DAD-MS-based rapid metabolome analyses of the extracts indicated the high influence of culture conditions on the secondary metabolite (SM) profiles. Among 69 compounds detected in all Penicillium sp. extracts, 46 were successfully dereplicated. Analysis of SM relatedness in culture conditions by Hierarchical Cluster Analysis (HCA) revealed generally low similarity and showed a strong effect of medium selection on chemical profiles of Penicillium sp. This is the first study assessing both the metabolite and bioactivity profile of the fungi associated with Baltic eelgrass Z. marina.

show abstract

Novel metabolites from Penicillium crustosum, including penitrem E, a tremorgenic mycotoxin

Cited by 39 publications

References 6 publications

Mycotoxic nephropathy in Bulgarian pigs and chickens: complex aetiology and similarity to Balkan Endemic Nephropathy

Mycotoxic nephropathy in Bulgarian pigs and chickens: complex aetiology and similarity to Balkan Endemic Nephropathy

Identification of novel metabolite and its cytotoxic effect on human lymphocyte cells in comparison to other mycotoxins

Rapid Metabolome and Bioactivity Profiling of Fungi Associated with the Leaf and Rhizosphere of the Baltic Seagrass Zostera marina

Contact Info

Product

Resources

About