Novel Metabolites Are Associated With Augmentation Index and Pulse Wave Velocity: Findings From the Bogalusa Heart Study

Abstract

Arterial stiffness measured by aortic pulse wave velocity (PWV) and augmentation index (AI) is a marker of subclinical organ damage and an independent risk factor for cardiovascular disease events and mortality. 1-5 Although many factors, including aging, diabetes, hypertension, dyslipidemia, and chronic kidney disease, contribute to arterial stiffening, the pathophysiological mechanisms of this process are still not fully understood. Circulating low-weight metabolites represent the intermediate and end products of metabolic pathways and may reflect initial stages of arterial stiffness. Recent advances in metabolomics technology have allowed for global characterization of a large panel of metabolites from biological samples, which provides a unique opportunity for investigating arterial stiffness mechanisms. Previous metabolomics studies have identified important metabolites associated with arterial stiffness, 6-8 revealing novel mechanisms of arterial stiffening. 6-8 However, these studies either had small sample sizes or only assayed a limited number of metabolites, and omitted metabolite networks. In this study, we performed untargeted metabolomics profiling using the most up-to-date metabolites panel in 1,239 participants of the Bogalusa Heart Study (BHS) to identify

Arterial Stiffness, Sugar-Sweetened Beverages and Fruits Intake in a Rural Population Sample: Data from the Brisighella Heart Study

“…Similarly, in prospective cohort studies, fructose-sweetened beverage consumption was associated with an increased risk of gout (relative risk = 2.08, 95%CI 1.40 to 3.08), while fruit intake was not [32]. In the Bogalusa Heart Study, serum fructose was associated with increased cfPWV [33]. These finding are in line with our observation that fructose-sweetened beverage intake is directly related tocfPWV.…”

Examination of serum metabolome altered by cigarette smoking identifies novel metabolites mediating smoking‐BMI association

“…Therefore, a total of 1,325 fasting serum samples were processed using an untargeted, ultra‐high‐performance liquid chromatography (HPLC)‐tandem mass spectroscopy‐based metabolomic quantification protocol. Detailed metabolomics profiling and quality control measures have been described previously (14). In brief, a technical replicate generated by taking a small fraction of every sample and a process blank were used to remove system artifacts, misassignments, and background noises.…”

“…A total of 1,202 metabolites were included in the analyses. There were 167 metabolites with a missing rate or below‐detection rate between 50% and 80%, and these metabolites were categorized as follows: 1 = missing or below detection limit; 2 = greater than detection limit but below median; and 3 = equal or greater than median (14). The rest of the metabolites ( n = 1,035) were normalized.…”

“…1 = missing or below detection limit; 2 = greater than detection limit but below median; and 3 = equal or greater than median (14). The rest of the metabolites (n = 1,035) were normalized.…”

Metabolomics of Arterial Stiffness

“…In a study by Jiang et al, human plasma SM levels were positively and independently related to CAD, and higher levels were proposed to be a potential marker for atherogenic remnant lipoprotein accumulation [ 138 ]. In the Bogalusa Heart Study, a metabolite module related to SM metabolism was associated with PWV in a biracial cohort of 1202 participants [ 139 ]. Another large study with 6814 middle-aged asymptomatic adults associated plasma SM levels with quantitative measures of subclinical atherosclerosis, although these associations were lost after adjustment for classical CV risk markers [ 140 ].…”