Novel messenger RNAs for body fluid identification

Albani, Patricia P.; Fleming, Rachel

doi:10.1016/j.scijus.2017.09.002

Cited by 35 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initial assays focused on identifying body fluid-specific messenger RNA (mRNA) markers, and development of multiplexes indicating the presence of single or multiple body fluid types, the latter of which is particularly useful when analysing mixed samples (reviewed in [55,60]). However, the susceptibility of mRNAs to degradation has limited their application to forensic samples, and mRNA assays also suffer from limitations including variation in sensitivity and specificity and interpretational challenges [61][62][63][64].…”

Section: Body Fluid Identificationmentioning

confidence: 99%

Developments in forensic DNA analysis

Haddrill

2021

Emerging Topics in Life Sciences

View full text Add to dashboard Cite

The analysis of DNA from biological evidence recovered in the course of criminal investigations can provide very powerful evidence when a recovered profile matches one found on a DNA database or generated from a suspect. However, when no profile match is found, when the amount of DNA in a sample is too low, or the DNA too degraded to be analysed, traditional STR profiling may be of limited value. The rapidly expanding field of forensic genetics has introduced various novel methodologies that enable the analysis of challenging forensic samples, and that can generate intelligence about the donor of a biological sample. This article reviews some of the most important recent advances in the field, including the application of massively parallel sequencing to the analysis of STRs and other marker types, advancements in DNA mixture interpretation, particularly the use of probabilistic genotyping methods, the profiling of different RNA types for the identification of body fluids, the interrogation of SNP markers for predicting forensically relevant phenotypes, epigenetics and the analysis of DNA methylation to determine tissue type and estimate age, and the emerging field of forensic genetic genealogy. A key challenge will be for researchers to consider carefully how these innovations can be implemented into forensic practice to ensure their potential benefits are maximised.

show abstract

Section: Body Fluid Identificationmentioning

confidence: 99%

Developments in forensic DNA analysis

Haddrill

2021

Emerging Topics in Life Sciences

View full text Add to dashboard Cite

show abstract

“…Lee et al 29 used Affymetrix gene chip for a comparison study on post-mortem samples on the mouse and repeated the same method for human., and found that Affymetrix gene chip can detect degraded RNA, as its unique detection from the 3` end of transcripts. 29 Similarly, Albani et al 82 characterized six novel mRNA for body fluids by using the Massively parallel sequencing (MPS)technique for the first time to search novel body fluid RNA markers.…”

Section: Microarraymentioning

confidence: 99%

Application of Ribonucleic Acid (RNA) in Forensic Sciences

Jawad¹,

Noreen²,

Ashraf³

et al. 2020

BIOMEDICA

View full text Add to dashboard Cite

Forensic science has been helping law enforcement agencies in better understanding and presenting the evidence in the court of law. In certain situations, when conventional forensic methods of investigations cannot make better conclusions with more specific accuracy, then molecular techniques do help in reaching the acquired accuracy in the results regarding the identification of evidence. Advanced molecular techniques, which are using Deoxyribose Nucleic acid (DNA), Ribose Nucleic Acid (RNA), and protein molecules to produce forensically important information from the samples recovered at the crime scene. DNA can only distinguish among individuals but is unable to discriminate the type of samples originated from the same sample. For this RNA has become a molecule of interest for its different levels of expression in different cells/tissues of an individual. RNA molecules of different types are being used to build up models for several purposes (injury-age, new-born age, molecular cause of death, etc.). Modern techniques like Real-time Polymerase Chain Reaction (PCR) and Microarray are being used for the detection of RNA molecules of interest both in the form of its abundance and as a unique molecular detection. This script will help in understanding the importance of RNA application in forensic sciences by providing an overview of the research done to date and the techniques being used for this purpose.

show abstract

“…Most commonly, these techniques take the form of multiplex reverse transcriptase endpoint PCR (RT‐PCR) (Akutsu, Watanabe, Takamura, & Sakurada, 2017; Albani & Fleming, 2018; Albani & Fleming, 2019; Fleming & Harbison, 2010a; Juusola & Ballantyne, 2005; Lindenbergh et al, 2012; Lindenbergh et al, 2013; Roeder & Haas, 2013). Although highly sensitive and specific, there is currently no reliable method for quantifying levels of RNA in forensic samples (Ingold et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

RNA‐based approaches for body fluid identification in forensic science

Lynch

Fleming

2020

WIREs Forensic Science

Self Cite

View full text Add to dashboard Cite

In forensic science, the identification of body fluids present in a stain can aid in the reconstruction of crime scenes and support activity-level information in some cases. RNA-based methods for body fluid identification address some limitations associated with conventional testing, including a lack of specificity and sample destruction. mRNA profiling by endpoint reverse transcription polymerase chain reaction (RT-PCR) is a confirmatory RNA method currently used by some forensic laboratories in casework due to the availability of capillary electrophoresis instruments. This method is highly specific and reliable when the RNA input is within the optimal range. When RNA input is below the limit of detection or too high, then false negatives and false positives/cross reactivity can occur, respectively. Real-time quantitative PCR (qPCR) is quantitative, more sensitive, and efficient than endpoint PCR, and does not require post-PCR processing. A reverse transcription (RT) step may also be included in qPCR (RT-qPCR), enabling mRNA quantification. However, there are concerns regarding the lack of consistency and standardization of RT-qPCR methodology and reporting of results. Additionally, the number of currently available fluorophores somewhat reduces multiplexing ability. More recently, approaches involving Piwi-interacting biomarkers, NanoString barcodes, and RNA sequencing have been developed. This review will cover the current and developing RNA-based techniques for forensic body fluid identification, focusing on endpoint and quantitative RT-PCR using mRNA. The advantages and limitations of these along with alternative methods will be discussed, and how they may be further developed to advance forensic body fluid identification.

show abstract

Novel messenger RNAs for body fluid identification

Cited by 35 publications

References 38 publications

Developments in forensic DNA analysis

Developments in forensic DNA analysis

Application of Ribonucleic Acid (RNA) in Forensic Sciences

RNA‐based approaches for body fluid identification in forensic science

Contact Info

Product

Resources

About