Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells

Mesimäki, Karri; Lindroos, Bettina; Törnwall, Jyrki; Mauno, J.; Lindqvist, Christer; Kontio, Risto; Miettinen, Susanna; Suuronen, Riitta

doi:10.1016/j.ijom.2009.01.001

Cited by 423 publications

(374 citation statements)

References 46 publications

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“…We decided to use ASCs mainly because of their great accessibility and low patient morbidity when compared with other mesenchymal cell sources such as bone marrow or periosteum. The in vitro, in vivo and clinical bone‐forming capacity of ASCs in combination with various scaffold materials have been reported by many authors 14, 20, 35, 36, 37, 38, 39, 40, 41. In this series, the flow cytometric characterization of the ASCs expanded in autologous serum corresponded to previously published results for ASCs 42, 43.…”

Section: Discussionsupporting

confidence: 86%

“…Granules also molded well to the defects. Moreover, there is prior evidence of the use of betaTCP in craniomaxillofacial surgery 20, 53, 54. The failure of the graft ossification of Patients 2 and 4 may be due to too rapid resorption of betaTCP granules which in turn may be related to the use of resorbable and less rigid supporting meshes in their cases.…”

Section: Discussionmentioning

confidence: 99%

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Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

Thesleff

Lehtimäki

Niskakangas

et al. 2017

Stem Cells Translational Medicine

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Several alternative techniques exist to reconstruct skull defects. The complication rate of the cranioplasty procedure is high and the search for optimal materials and techniques continues. To report long‐term results of patients who have received a cranioplasty using autologous adipose‐derived stem cells (ASCs) seeded on beta‐tricalcium phosphate (betaTCP) granules. Between 10/2008 and 3/2010, five cranioplasties were performed (four females, one male; average age 62.0 years) using ASCs, betaTCP granules and titanium or resorbable meshes. The average defect size was 8.1 × 6.7 cm2. Patients were followed both clinically and radiologically. The initial results were promising, with no serious complications. Nevertheless, in the long‐term follow‐up, three of the five patients were re‐operated due to graft related problems. Two patients showed marked resorption of the graft, which led to revision surgery. One patient developed a late infection (7.3 years post‐operative) that required revision surgery and removal of the graft. One patient had a successfully ossified graft, but was re‐operated due to recurrence of the meningioma 2.2 years post‐operatively. One patient had an uneventful clinical follow‐up, and the cosmetic result is satisfactory, even though skull x‐rays show hypodensity in the borders of the graft. Albeit no serious adverse events occurred, the 6‐year follow‐up results of the five cases are unsatisfactory. The clinical results are not superior to results achieved by conventional cranial repair methods. The use of stem cells in combination with betaTCP granules and supporting meshes in cranial defect reconstruction need to be studied further before continuing with clinical trials. Stem Cells Translational Medicine 2017;6:1576–1582

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

Thesleff

Lehtimäki

Niskakangas

et al. 2017

Stem Cells Translational Medicine

View full text Add to dashboard Cite

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“…By fostering collaborative relationships between teams from academia, biotechnology startups, and established biomaterial industry companies, the development of ASC-based regenerative medical therapies for cartilage and bone defects can be accomplished in compliance with existing federal and international regulatory requirements. Evidence already exists suggesting that these goals are feasible since the successful use of autologous ASCs to repair critical-sized craniofacial defects has been published as case reports in the clinical literature [52,58].…”

Section: Discussionmentioning

confidence: 99%

2010 Nicolas Andry Award: Multipotent Adult Stem Cells from Adipose Tissue for Musculoskeletal Tissue Engineering

Guilak

Estes

Diekman

et al. 2010

Clinical Orthopaedics &Amp; Related Research

141

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Background Cell-based therapies such as tissue engineering provide promising therapeutic possibilities to enhance the repair or regeneration of damaged or diseased tissues but are dependent on the availability and controlled manipulation of appropriate cell sources. Questions/purposes The goal of this study was to test the hypothesis that adult subcutaneous fat contains stem cells with multilineage potential and to determine the influence of specific soluble mediators and biomaterial scaffolds on their differentiation into musculoskeletal phenotypes. Methods We reviewed recent studies showing the stemlike characteristics and multipotency of adipose-derived stem cells (ASCs), and their potential application in cellbased therapies in orthopaedics.

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“…There is evidence that functional ASCs can be expanded directly from lipoaspirate fluids without the need for collagenase digestion [14]. Similarly, multiple groups routinely use porcine-derived trypsin to passage plastic adherent ASCs, and recent studies have documented the equivalent performance of bacterial-derived or corn-derived trypsin products [15][16][17]. Thus, the removal of enzyme reagents is achievable.…”

Section: Enzyme Qualitymentioning

confidence: 99%

“…A number of laboratories have found that human serum or platelet-derived supplements can serve as alternatives (reviewed in [20]). Some groups have relied on autologous serum, donated by the subject at the time of tissue collection, for ASC expansion [16]. The future may witness the development of commercial grade, infectious agent-free allogeneic serum sources for the generation of cGMP cell products.…”

Section: Serum Alternativesmentioning

confidence: 99%

Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let's Not Get Lost in Translation

et al. 2011

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Subcutaneous fat has emerged as an alternative tissue source for stromal/stem cells in regenerative medicine. Over the past decade, international research efforts have established a wealth of basic science and preclinical evidence regarding the differentiation potential and regenerative properties of both freshly processed, heterogeneous stromal vascular fraction cells and culture expanded, relatively homogeneous adipose-derived stromal/stem cells. The stage has been set for clinicians to translate adipose-derived cells from the bench to the bedside; however, this process will involve “development” steps that fall outside of traditional “hypothesis-driven, mechanism-based” paradigm. This concise review examines the next stages of the development process for therapeutic applications of adipose-derived cells and highlights the current state of the art regarding clinical trials. It is recommended that the experiments addressing these issues be reported comprehensively in the peer-review literature. This transparency will accelerate the standardization and reproducibility of adipose-derived cell therapies with respect to their efficacy and safety.

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Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells

Cited by 423 publications

References 46 publications

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

2010 Nicolas Andry Award: Multipotent Adult Stem Cells from Adipose Tissue for Musculoskeletal Tissue Engineering

Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let's Not Get Lost in Translation

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