Novel materials to enhance corneal epithelial cell migration on keratoprosthesis

Karkhaneh, Akbar; Mirzadeh, Hamid; Ghaffariyeh, Alireza; Ebrahimi, Aziz; Honarpisheh, Nazafarin; Hosseinzadeh, Masud; Heidari, Mohammad Hossein

doi:10.1136/bjo.2009.178632

Cited by 19 publications

(11 citation statements)

References 16 publications

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“…Since 1878, a variety of materials such as autologous and allografts, paper patches, synthetic and bioabsorbable materials, composite materials, and, lately, cell‐seeded and tissue‐ engineered grafts have been used for TM grafting 3–9. The current concern is which composite materials and biomaterials are the best bases and scaffolds regarding high susceptibility to support cell adhesion, proliferation, and differentiation, as well as their ability to promote tissue repair in vivo and consequently repair the perforation 6, 10–14. Polydimethyl siloxane (PDMS) is one of these preferred biomaterial patches.…”

Section: Introductionmentioning

confidence: 99%

Collagen‐immobilized patch for repairing small tympanic membrane perforations: In vitro and in vivo assays

Farhadi

Mirzadeh

Solouk

et al. 2011

J Biomedical Materials Res

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Tympanic membrane (TM) perforation is still one of the most common otology complications. New designs of biomaterials, and lately tissue-engineered composites and grafts, have thoroughly revolutionized the management of TM perforation. In this study, we examined a biologically modified collagen-immobilized polydimethyl siloxane patch to repair TM perforation. In vitro potential of the aforementioned patch as a scaffold to support fibroblast cell growth and adhesion was assessed. An in vivo assay of the patch for initiating repair of TM perforations also was investigated. In vitro assay showed that the patch has significantly increased cell adhesion and growth in comparison with unmodified ones (p < 0.05). In vivo study also showed an overall closure rate of TM perforation of 70% and an average gain of 15.75 ± 4.29 dB in air-bone gap. This study shows that the preliminary in vivo evaluation of a modified siloxane patch in humans had promising results and is comparable to existing biomaterial patches.

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Section: Introductionmentioning

confidence: 99%

Collagen‐immobilized patch for repairing small tympanic membrane perforations: In vitro and in vivo assays

Farhadi

Mirzadeh

Solouk

et al. 2011

J Biomedical Materials Res

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“…Epithelial cells have been cultured on substrates such as amniotic membrane and silk, as well as specially treated plastic. A recent study in rabbits using a keratoprosthesis with a silicone optical core suggests that coating the prosthesis with type I collagen improves epithelial cell adherence and migration [22]. Our patients were not distinguished by race, gender, age, perioperative medications, or underlying ophthalmic diagnosis as compared to other keratoprosthesis patients.…”

Section: Discussionmentioning

confidence: 69%

Surface epithelialization of the type I Boston keratoprosthesis front plate: immunohistochemical and high-definition optical coherence tomography characterization

Kiang

Rosenblatt

Sartaj

et al. 2012

Graefes Arch Clin Exp Ophthalmol

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BackgroundThe aim of this work is to characterize a transparent tissue layer partially covering the anterior surface of the type I Boston permanent keratoprosthesis front plate in four patients.MethodsThe tissue over the front plate was easily scrolled back as a single transparent layer using a sponge. In two cases, histopathologic analysis was undertaken and immunofluorescent staining with a cytokeratin 3-specific antibody was performed. The relationship of the tissue to the keratoprosthesis device was further characterized using spectral domain high-definition optical coherence tomography (HD-OCT).ResultsHistopathologic analysis revealed the tissue to be non-keratinized squamous epithelium. No goblet cells were seen, suggesting the cells were of corneal, and not conjunctival, epithelial origin. Immunofluorescent staining of all cells was positive for cytokeratin 3, a protein strongly associated with corneal epithelium. The tissue was easily discerned by HD-OCT and was of substantial thickness near the external junction between the keratoprosthesis device and the carrier corneal tissue. In three cases, visual acuity was unaffected by the presence or absence of this tissue. In one case, a prominent tissue margin temporarily obscured the visual axis and reduced visual acuity; this resolved with mechanical central debridement and has not recurred.ConclusionsThe transparent tissue layer covering the anterior surface of the type I Boston keratoprosthesis front plate was found to represent non-keratinized squamous epithelium, most likely of corneal epithelial origin. This potentially represents a further step in bio-integration of the keratoprosthesis device. In particular, epithelial coverage of the critical junction between the device and the carrier corneal tissue might serve an important barrier function and further reduce the incidence of infection and extrusion of the type I Boston permanent keratoprosthesis.

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“…To improve the blood compatibility of biomaterials, various techniques of surface modification have been applied in order to alter the surface properties without affecting the bulk ones (Dadsetan et al2001aKarkhaneh et al 2010Solouk et al2011a). Among these techniques which have been developed for this purpose, laser irradiation (Mirzadeh et al19932011Khorasani and Mirzadeh 2004bMirzadeh and Bagheri 2007) and plasma treatment (Chu 2007Mochizuki et al2011Solouk et al2011ab) can be applied to create new functional groups, micro- and nanostructures, and change in surface wettability.…”

Section: Introductionmentioning

confidence: 99%

Polystyrene surface modification using excimer laser and radio-frequency plasma: blood compatibility evaluations

Bagheri‐Khoulenjani¹,

Mirzadeh²

2012

Prog Biomater

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Biomaterial surface modification is an efficient method to improve and control blood component-material interactions. In the present study, two different methods (ArF excimer laser irradiation and radio-frequency (RF) plasma treatment) were applied in separate procedures to create a vast range of physicochemical characteristics on the surface of polystyrene (PS) and investigate their effects on blood compatibility of treated surfaces. Atomic force microscopy (AFM) and Fourier transmission infrared analysis were applied to study the morphology and chemical characteristics of treated samples in comparison with those of the untreated PS. Contact angle and surface tension measurements with two different solvents were used to evaluate the wettability and surface energy of the treated PS films. The effect of the physicochemical properties of the PS surface on blood compatibility was investigated using lactate dehydrogenase (LDH) method. AFM studies showed that after laser treatment, some distinctive nanostructures are created on the surface of PS. The data from contact angle measurements demonstrated that ArF excimer laser irradiation and RF plasma treatment created surfaces with a vast range of properties in the wettability point of view. The LDH results revealed that after surface modification by laser irradiation and plasma treatment, blood compatibility of PS films was enhanced. In addition, these results offered that the most blood compatible samples were those which irradiated with 5 pulses of laser and the one treated 4 minutes in oxygen plasma.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-1-4) contains supplementary material, which is available to authorized users.

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Novel materials to enhance corneal epithelial cell migration on keratoprosthesis

Abstract: This method may have potential use in silicone optical core Keratoprosthesis.

Cited by 19 publications

References 16 publications

Collagen‐immobilized patch for repairing small tympanic membrane perforations: In vitro and in vivo assays

Collagen‐immobilized patch for repairing small tympanic membrane perforations: In vitro and in vivo assays

Surface epithelialization of the type I Boston keratoprosthesis front plate: immunohistochemical and high-definition optical coherence tomography characterization

Polystyrene surface modification using excimer laser and radio-frequency plasma: blood compatibility evaluations

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