Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

Abstract

Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGFconjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a threedimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin-and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

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Inulin nanoparticles and silymarin counteract chlorpromazine-induced injury in the liver and kidney of rats

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Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

“…Different ways to ensure extended availability of added NTFs at the site 2,3 stefano geuna 1 of nerve reconstruction have been attempted in recent years, including gene therapy via transplanted Schwann cells 4 or nanotechnology approaches. 5 Ex vivo gene therapy can be used to genetically induce the overexpression of selected NTFs in cells that are later transplanted as part of tissue-engineered nerve grafts. 6 The usefulness of this approach has been proven already for transplanted Schwann cells overexpressing different isoforms of fibroblast growth factor-2 (FGF-2) in the rat sciatic nerve model.…”