Novel lytic bacteriophages of Klebsiella oxytoca ABG-IAUF-1 as the potential agents for mastitis phage therapy

Abstract

Mastitis is an inflammation of the mammary gland that occurs when pathogenic microorganisms enter the udder. Even though tremendous advancements in veterinary diagnosis and therapeutics, mastitis is still the most frequent and costly disease of dairy herds overall the world. The purpose of this research was to isolate and identify the lytic phages as a potential method for biological control of bovine mastitis. In this study Klebsiella oxytoca was isolated from contaminated milk samples of Isfahan dairy herds, Isfahan, Iran and characterized as K. oxytoca ABG-IAUF-1 and its 16s-rRNA sequence was deposited in GenBank under the accession numbers of MF175803.1. Then, the four novel specific lytic bacteriophages of K. oxytoca ABG-IAUF-1 from Isfahan public wastewater were isolated and identified. The results of transmission electron microscopy indicated that theses isolated phages were related to Myoviridae and Podoviridae families of bacteriophages. Also the analysis of the growth curve of K. oxytoca ABG-IAUF-1 before and after treatment with lytic phage showed the 97% success rate of the phages in preventing of bacterial growth. This is the first report indicating the use of bacteriophages as the potential agents for eliminating the pathogenic bacteria responsible for bovine mastitis in Iran. The applications of these lytic phages could be an asset for biocontrolling of pathogenic agents in medical and veterinary biotechnology.

Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review

“…They have the inherent ability to target and destroy specific bacterium and also the capacity to replicate exponentially which makes them a potential candidate against pathogenic bacteria (Carson et al 2010 ). Several bacteriophages have been isolated and studied for their potential in eliminating pathogenic bacteria associated with mastitis (Dias et al 2013 ; Porter et al 2016 ; Amiri Fahliyani et al 2018 ; Varela-Ortiz et al 2018 ). Several potential candidates have been identified for bacteriophage therapy against S. aureus (Dias et al 2013 ; Varela-Ortiz et al 2018 ) , Klebsiella oxytoca (Amiri Fahliyani et al 2018 ) and E. coli (Porter et al 2016 ).…”

A novel thermo- ethanol tolerant Acetobacter okinawensis KBMNS-IAUF-1 isolated from Iranian nectarine as a potential for nectarine vinegar production in food biotechnology

“…The final steps were 72°C for 4 minutes and incubation at 4°C for 10 minutes. The expected molecular weight of PCR product was 370 bp [21,27]. The PCR product and primers were sent to Taligene Pars Co., Isfahan Science and Technology Town (ISTT), Isfahan, Iran for DNA sequencing.…”

“…Among AAB, Acetobacter spp. As the main responsible microorganism for vinegar production have been isolated from several natural resources such as grape, date and coconut [16][17][18], Iranian white-red cherry [19,20], Iranian peach [1,21], Iranian apricot [22], Jamaican cherry, pineapple, rambutan, mango and longan [23,24], palm wine and palm tree [25][26][27] and Iranian date palm, Rotab [5]. The aims of this research were isolation and identification of AAB from Iranian nectarine as well as investigation of their tolerance against high temperatures and ethanol concentrations.…”

Molecular Characterization of Sewage Bacteriophage and Their Efficiency in Treatment of Antibiotic Resistant Bacteria

“…In order to confirm the presence of phages, 10 μL of phage filtrate was dropped on BHA 's bacterial lawns and then incubated for 24 hours at 37•C. [7] .…”