Novel Liposomal Formulation for Targeted Gene Delivery

Abstract

The present data suggest that our new PSIL formulation combines molecular features required for targeted gene therapy including high DNA encapsulation efficiencies and vector-specific transient transfection capacity.

Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

“…The MTS reagent, 3-[4, 5-dimethylthiazol-2-yl]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, was purchased from Promega Corporation (Madison, WI). Anti-integrin antibodies (α V β 3 , MAB1976; α 5 β 1 , MAB1969; α 5 , CBL497) were obtained from 17 Briefly, DSPE-PEG(2000) maleimide ammonium salt (molecular weight 2941.61) and RGD peptide were dissolved in a buffer solution (50 mM triethanolamine hydrochloride, 50 mM sodium phosphate, 150 mM NaCl, and 1 mM EDTA, pH 8). DSPE-PEG(2000) maleimide solution (2 mM) was gently mixed with an equal volume of RGD peptide solution (4 mM) overnight in a glass bottle at 4°C.…”

“…29 However, previous studies have usually prepared the nanoparticles first, and then added a targeting molecule for conjugation on the surface of the nanoparticle. 17,29,30 This method is limited by the difficulty in controlling the number of targeting moieties conjugated to the constructed nanoparticles. In this report, synthesis of the ligand-conjugated lipid, DSPE-PEG-RGD, was performed by Michael addition reactions between activated maleimide and thiol groups prior to preparation of the liposomes ( Figure 1A).…”

Targeted Nanoparticles for Cancer Therapy

“…Thus, antibodies and antibody fragments against the TfR are common examples of receptor targets [72]. Among these are the mAbs A24 [96], Rat anti-murine TfR RI7 217 and YE1/9.9, Murine anti-human TfR Antibody HB21(also known as 5E9), Antibody 454A12, Antibody B3/25, Antibody OKT9, R17217 and OX26 mAb [95,[97][98][99].…”

Nanomicelle‐Assisted Targeted Ocular Delivery with Enhanced Antiinflammatory Efficacy In Vivo

“…For example, liposomes prepared with DSPE‐PEG 2000 and helper lipids were a popular kind of nonviral ocular gene carrier 20, 21. DSPE‐PEG 2000 contained liposomes with conjugated cRGD ligand were proved to be efficient vectors for retinal cells when loaded with VEGF‐siRNA 22, 23.…”