Novel lipases discovery specifically from marine organisms for industrial production and practical applications

Navvabi, Azita; Razzaghi, Mozhgan; Fernandes, Pedro; Karami, Leila; Homaei, Ahmad

doi:10.1016/j.procbio.2018.04.018

Cited by 66 publications

(21 citation statements)

References 138 publications

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“…The potential of application for lipases is noticeable in the production of biofuels, leather, foods, detergents, cosmetics, fragrances, and pharmaceuticals [68,69]. Moreover, they are easily obtained from a diversity of biological sources by extraction from animal and plant tissues or cultivation of microorganisms, providing enzymes with different substrate specificities and catalytic properties [66,70]. Most lipases utilized as catalysts in organic synthesis are of microbial and fungal origin, such as Candida rugosa, Candida antarctica, Pseudomonas fluorescens, Rhizopus oryzae, Burkholderia cepacia, Aspergillus niger, Thermomyces lanuginosus, and Rhizomucor miehei, being easy to obtain by fermentation and a basic purification step [71].…”

Section: Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.

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Section: Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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“…Lipase production belongs to one of the dominated industrial enzyme productions [4]. Lipases can be used in the production of fine chemicals, detergents, in paper manufacture, feed and food industries, leather industry, medicine, and especially in biodiesel production [5]. The big economic aspect for applying lipases in industrial processes is its need to recover and recycle, whereas significant researches are dedicated to the investigations of immobilization techniques.…”

Section: Introductionmentioning

confidence: 99%

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

et al. 2019

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This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 °C, t = 9 days). Lipase showed optimum activity at T = 40 °C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 °C. During the experiments, the operational stability of the enzyme was studied. The obtained results clearly showed that produced crude and purified lipase can be used for biodiesel production, but the process needs some additional optimization. As for operation stability, it was noticed that the commercial enzyme was deactivated after 30 h, while produced crude enzyme remained 8.25% of its activity after 368 h.

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“…When using lipases as biocatalysts in biodiesel production, those problems can be minimized or even eliminated. 8 Lipase can be used as immobilized 9,10 or free 11,12 originating from different organisms such as filamentous fungi, 13 bacteria and yeasts, 14 marine organisms, 15 plants and animals. 16 It should be noted here that, during the biocatalytic production of biodiesel, there are no other by-products except glycerol, and thus the purification of such biodiesel is easier to perform compared to the biodiesel produced by chemically catalysed transesterification.…”

Section: Biodiesel Productionmentioning

confidence: 99%

Membranska filtracija kao ekološki prihvatljiva metoda pročišćavanja sirovog biodizela

Ostojčić

Brkić²,

Tišma

et al. 2020

Kem. ind. (Online)

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Biodizel je prvo alternativno gorivo čija su fizikalno-kemijska svojstva regulirana odgovarajućim standardima: američkim ASTM D 6751 i europskim standardom EN 14214. Proces proizvodnje biodizela sastoji se od tri glavne faze: 1) pripreme sirovine, 2) transesterifikacije i 3) obrade produkta reakcije – pročišćavanje sirovog biodizela kako bi se zadovoljile specifikacije koje su navedene u prethodno spomenutim standardima. Proces pročišćavanja sirovog biodizela obično se provodi dvjema tehnikama: vlažnim i suhim pranjem. Najčešće primjenjivani postupak je mokro pranje. Glavni nedostatak u upotrebi vode u procesu pročišćavanja je stvaranje velike količine otpadne vode koja uvelike povećava troškove proizvodnje biodizela nakon čega slijedi sušenje proizvoda, što zahtijeva dodatni utrošak energije i vremena. Najveći nedostatak suhog pranja s različitim ionsko-izmjenjivačkim smolama je nemogućnost uklanjanja glicerola i metanola iz sirovog biodizela do onih granica koje su propisane EN 14214 te problem odlaganja iskorištenih ionsko-izmjenjivačkih smola. Zbog toga se kao alternativa postojećim tehnikama pročišćavanja pojavila primjena membranske tehnologije u procesu pročišćavanja biodizela. Membranska filtracija je ekološki prihvatljiva i zahtijeva manje energije. Membranskom filtracijom može se smanjiti udio glicerola, metanola i vode u biodizelu do količina propisanih standardima. U okviru ovog rada prikazan je kratki pregled mogućnosti primjene ultra i/ili mikrofiltracije u procesu pročišćavanja biodizela.

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Novel lipases discovery specifically from marine organisms for industrial production and practical applications

Cited by 66 publications

References 138 publications

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

Membranska filtracija kao ekološki prihvatljiva metoda pročišćavanja sirovog biodizela

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