Novel Lipase Reactor based on Discontinuous Interfaces in Hydrogel-Organogel Hybrid Gel: A Preliminary Exploration

Abstract

According to the "interfacial activation" mechanism, constructing a sufficient interface is the key strategy for lipasecatalytic system designing. Based on the "infinite interface in finite three-dimensional space" logic, in the current study, poly(N,Ndimethylacrylamide) (PDMA)-polybutyl methacrylate (PBMA) hybrid gels were prepared by a two-step crosslinking strategy, subsequently constructed as lipase-interfacial catalytic systems. The results confirm that the PDMA-PBMA hybrid gels with "networks in pores" structures could swell both the aqueous phase and organic phase. The balance between water swelling and isooctane swelling, hybrid gel space (height control), and the lipase entry manner significantly affect the interface construction and consequently the catalytic efficiency. The enzyme−substrate contact rate affected by swelling leads to three catalytic stages. Considering the spatial barrier and distribution of lipases, a potential high-performance lipase reactor can be assembled from smallsize, lamellar-like, and porous hybrid gels. The reactors also show good time storage and low temperature tolerance.

Folin–Ciocalteu Reagent-Loaded Acrylamide-Based Hydrogel Sensor for Antioxidant Capacity Measurement with the Molybdenum Green Method

“…In our study, the molybdenum-green method was carried out on a solid material surface, and the FC reagent was turned into a sensor for the first time. The reaction with antioxidants proceeded at room temperature without any heating and did not require any agitation owing to interfacial catalysis provided by the hydrogel; 38 the reaction time was 15 min, and it was possible to work with acidic samples (e.g., fruit juice, vinegar). This method may be more selective for true antioxidant compounds, unlike the classical FC assay that responds to many weak reductants, such as amino acids, sugars, and monohydric phenols.…”

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

“…For enzyme preparations, it is not only the immobilization conditions that need to be considered; the selection of immobilization material, activation condition, and reactor are also important factors, − which should have different degrees of influence on the properties of immobilized lipase. Therefore, while AI technology is utilized to computationally optimize immobilization conditions, it can also facilitate the rational design of immobilized enzymes with improved performance, accelerating the discovery of more efficient and sustainable catalysts for various biotechnological applications.…”

Short Peptides for Hydrolase Supramolecular Mimicry and Their Potential Applications

“…They have also been immobilized on a substrate for improved stability and for the ease of recycling [ 45 , 46 ]. In particular, the use of gel phases to immobilize lipases for enhanced performance has been reported, including organogels [ 47 ], emulsion gels [ 48 ], hydrogel microspheres [ 49 , 50 ], ionic gels [ 51 ], discontinuous hydrogel-organogel hybrids [ 52 ], and composite gels [ 53 ].…”