Novel Laboratory Mouse Papillomavirus (MusPV) Infection

Ingle, Arvind; Ghim, Shin-je; Joh, Joongho; Chepkoech, I.; Jenson, A. Bennett; Sundberg, John P.

doi:10.1177/0300985810377186

Cited by 110 publications

(152 citation statements)

References 21 publications

Supporting

Mentioning

140

Contrasting

Order By: Relevance

“…For each preparation, lesions first became visible about 1 month after inoculation, continued to grow progressively, and did not regress spontaneously, even when some mice were observed for more than 9 months. The results with the homologous quasivirions were similar to those described initially for MusPV1 (13).…”

Section: Generation Of a Synthetic Full-length Muspv1 Genome In Vitrosupporting

confidence: 86%

“…A recent report has confirmed the ability of the MusPV1 genome to induce papillomas in Foxn1 nu /Foxn1 nu mice (15). The presence of large arrays of virus particles in lesional keratinocytes (13,14), together with the recent identification of an MusPV1 variant, displaying 99% nucleotide identity to the original MusPV1, in normal skin from a German house mouse (8), supports the conclusion that MusPV1 is a bona fide domestic mouse virus.…”

mentioning

confidence: 71%

“…The in vivo infectivities of these three preparations in immunodeficient, athymic NCr nude mice were evaluated by inoculating their muzzles, the location originally described for the spontaneous MusPV1-induced papillomas (13), and following the animals for 3 months.…”

Section: Generation Of a Synthetic Full-length Muspv1 Genome In Vitromentioning

confidence: 99%

“…Therefore, the report of the first known PV that can cause lesions in the domestic mouse (Mus musculus) represented a potentially important advance (13). The virus was identified in an immunodeficient, inbred NMRI-Foxn1 nu /Foxn1 nu nude mouse colony and was termed MusPV1.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Handisurya

Day

Thompson

et al. 2013

J Virol

View full text Add to dashboard Cite

Full-length genomic DNA of the recently identified laboratory mouse papillomavirus 1 (MusPV1) was synthesized in vitro and was used to establish and characterize a mouse model of papillomavirus pathobiology. MusPV1 DNA, whether naked or encapsidated by MusPV1 or human papillomavirus 16 (HPV 16) capsids, efficiently induced the outgrowth of papillomas as early as 3 weeks after application to abraded skin on the muzzles and tails of athymic NCr nude mice. High concentrations of virions were extracted from homogenized papillomatous tissues and were serially passaged for >10 generations. Neutralization by L1 antisera confirmed that infectious transmission was capsid mediated. Unexpectedly, the skin of the murine back was much less susceptible to virion-induced papillomas than the muzzle or tail. Although reporter pseudovirions readily transduced the skin of the back, infection with native MusPV1 resulted in less viral genome amplification and gene expression on the back, including reduced expression of the L1 protein and very low expression of the L2 protein, results that imply skin region-specific control of postentry aspects of the viral life cycle. Unexpectedly, L1 protein on the back was predominantly cytoplasmic, while on the tail the abundant L1 was cytoplasmic in the lower epithelial layers and nuclear in the upper layers. Nuclear localization of L1 occurred only in cells that coexpressed the minor capsid protein, L2. The pattern of L1 protein staining in the infected epithelium suggests that L1 expression occurs earlier in the MusPV1 life cycle than in the life cycle of high-risk HPV and that virion assembly is regulated by a previously undescribed mechanism.

show abstract

Section: Generation Of a Synthetic Full-length Muspv1 Genome In Vitrosupporting

confidence: 86%

mentioning

confidence: 71%

Section: Generation Of a Synthetic Full-length Muspv1 Genome In Vitromentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Handisurya

Day

Thompson

et al. 2013

J Virol

View full text Add to dashboard Cite

show abstract

“…In 2011, Ingle et al reported the isolation of a new mouse papillomavirus (9). This virus was found in lesions on the face of animals in a colony of NMR1-Foxn1 nu /Foxn1 nu mice in India and was subsequently isolated and passaged.…”

mentioning

confidence: 99%

Secondary Infections, Expanded Tissue Tropism, and Evidence for Malignant Potential in Immunocompromised Mice Infected with Mus musculus Papillomavirus 1 DNA and Virus

Cladel

Budgeon

Cooper

et al. 2013

J Virol

View full text Add to dashboard Cite

Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce virus from DNA infections in immunocompromised mice. In these early studies, we have demonstrated both primary and secondary infections, expanded tissue tropism, and extensive dysplasia. Papillomaviruses are double-stranded DNA tumor viruses of about 8 kb. These viruses are ubiquitous in nature, and 241 types have been identified to date in both humans and many other animal species (http://pave.niaid.nih.gov). A subset of human papillomaviruses is linked to cancers, most notably cervical cancer, but also cancers of the head and neck, skin, and other anogenital sites. Papillomaviruses require fully differentiating cells for the completion of the viral life cycle and thus are not easily studied in cell culture systems. For a review of these viruses, see reference 1. The cottontail rabbit papillomavirus (CRPV) in vivo model has proven to be very useful in our hands for the study of cutaneous papillomavirus disease (2-8). An in vivo system to study both cutaneous and mucosal disease, papillomavirus pathology, and

show abstract

Papillomaviruses

Roberts

2015

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Papillomaviruses are a large family of small deoxyribonucleic acid (DNA) tumour viruses that cause hyperproliferative warts of cutaneous and mucosal epithelium. These viruses are ubiquitous in the animal kingdom and infect reptiles, birds and mammals and probably originated 350 million years ago. Subsets of human papillomaviruses (HPVs), referred to as ‘high‐risk’ types are associated with anogenital cancers (uterine cervix, vulva, vagina, anus, penis), oropharyngeal cancers and skin cancers. The papillomavirus life cycle is strictly dependent on the terminal differentiation programme of the host cell – the keratinocyte. The virus deregulates host cell cycle control and inactivates the host cells' antiproliferative response in order to reprogramme the differentiating cells to support viral replication. In the case of high‐risk viruses, this is achieved by interactions with important tumour repressor pathways. Two prophylactic vaccines (Gardasil and Cervarix) are available to control infection by the most prevalent HPV types found in anogenital and oropharyngeal cancers. Key Concepts Papillomaviruses show strong species and tissue restriction and they share a similar genetic organisation. Papillomaviruses are small double‐stranded (ds) DNA viruses that replicate as non‐integrated episomes in keratinocytes and are dependent on the keratinocyte undergoing terminal differentiation in order to complete their life cycle. Low‐risk viruses such as HPV6 and 11 are associated with the formation of anogenital warts and laryngeal papillomaviruses that have a low risk of progression to cancer. The most severe impact of human papillomavirus infection is the progression of a small proportion of infections to anogenital, oropharyngeal and skin cancers. High‐risk viruses such as HPV16 and 18 are associated with infections of the mucosa lining the anogenital and oropharyngeal tract and HPV16 is the most prevalent genotype in cancers arising at these sites. A majority of HPV infections are naturally eliminated by host‐cell‐mediated immunity and HPV infections are especially common in immunocompromised individuals (e.g. HIV‐infected patients, organ‐recipient patients, predisposing genetic conditions, e.g. epidermodysplasia verruciformis). These viruses are a common sexually transmitted disease (STD) and infection in the oropharynx is most likely via an oral‐genital route. High‐risk HPV early proteins E5, E6 and E7 are oncoproteins and the expression of E6 and E7 is retained in all cancers; E6 and E7 deregulate tumour suppressor pathways leading to host genomic instability. Papillomaviruses replicate as extrachromosomal plasmids in the nulei of infected cells, but during cancer progression, the viral genome often becomes integrated into the host chromosome, resulting in a loss of the negative feedback control of viral oncogene expression by E2. Prophylactic vaccines protect against infection with vaccine‐associated HPV types by inducing neutralising antibodies that recognise epitopes on the major capsid protein.

show abstract

Novel Laboratory Mouse Papillomavirus (MusPV) Infection

Cited by 110 publications

References 21 publications

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Secondary Infections, Expanded Tissue Tropism, and Evidence for Malignant Potential in Immunocompromised Mice Infected with Mus musculus Papillomavirus 1 DNA and Virus

Papillomaviruses

Contact Info

Product

Resources

About