Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms

Palacios, Irene; García–Lafuente, Ana; Guillamón, Eva; Villares, Ana

doi:10.1016/j.carres.2012.06.016

Cited by 83 publications

(37 citation statements)

References 27 publications

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“…The occurrence of different structural patterns between polysaccharides from Pleurotus species has been previously observed, for instance, a linear α-(1→3)-linked D-glucan has been isolated from P. ostreatus and P. eryngii [64], and a β-(1→3),(1→6)-linked glucan has been extracted from P. ostreatus [65]. Furthermore, the mushroom Pleurotus florida has shown different types of glucans, which are a water-soluble αβ-polysaccharide consisting of a main chain of α-(1→3)-linked D-glucan partially substituted at O-3 and O-6 by β-D-glucose [66] and a water-insoluble (1→3),(1→6)-linked β-D-glucan [67].…”

Section: Structural Features Of Polysaccharides Occurring In Mushroomsmentioning

confidence: 76%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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Abstract:Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

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Section: Structural Features Of Polysaccharides Occurring In Mushroomsmentioning

confidence: 76%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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“…[25] with modifications. Dried and powdered fruit bodies were steeped with ethanol (approximately 10 volume) for 2 days to eliminate the alcohol soluble components and filtered residue was re-extracted with ethanol.…”

Section: Methodsmentioning

confidence: 99%

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

et al. 2017

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Mushrooms have been prized by humankind as medicine and culinary wonder since antiquity. Though several species are ethnically valued; many prospective species are still being discovered. One such wild macrofungus has recently been discovered during subsequent field surveys in West Bengal, India which in turn exposed as a traditionally consumed popular myco-food. The collected taxon was found to be unique with regard to its morphological as well as genetical features. After detailed characterizations, the fungus was identified as a novel taxon belonging to the genus Russula (Russulaceae, Basidiomycota). Besides, the investigation was further extended in search of new functional ingredients and in this context, a water soluble crude polysaccharide rich extract (Rusalan) was isolated from dried basidiocarps. Accumulating evidences from GC-MS, HPTLC, FT-IR along with several spectrophotometric methods postulated that the fraction consisted mainly of carbohydrate in triple helical conformation, where glucose was the major monosaccharide mostly with β-type glycosidic linkage. Conversely, Rusalan showed pronounced antioxidant activity in six in vitro assay systems with EC50 value ranging from 190–1328 μg/ml concentration. The crude polysaccharide was also evaluated against six bacterial strains using microdilution method and the growth of Staphylococcus aureus and Bacillus subtilis were found to be inhibited effectively. In addition, immune-stimulatory assays demonstrated that Rusalan could evidently promote proliferation, induce phagocytosis, release NO, produce intracellular ROS and upregulate mRNA expression of iNOS, TNF-α, COX-2, as well as IL-6 genes in in mouse macrophage cells. Therefore, aim of the present study was not only to describe a new taxon to the world mycoflora but also to introduce a potent therapeutic agent that could be explored for food and pharmaceutical purposes. However, isolation of active component and in vivo studies need to be designed further.

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“…The extraction procedure was followed according to Palacios et al (2012) with modifications. Dried and powdered fruit bodies were steeped with 10 volume of 99 % ethanol for 2 days at room temperature to extract the alcohol soluble constituents.…”

Section: Extraction Of Polysaccharidementioning

confidence: 99%

Influence of extraction parameters on physico-chemical characters and antioxidant activity of water soluble polysaccharides from Macrocybe gigantea (Massee) Pegler & Lodge

Khatua

Acharya

2015

J Food Sci Technol

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Polysaccharides from mushrooms are potentially active pharmaceutical ingredients and their action is dependent upon conformation, composition, size etc. In this context, three water soluble crude polysaccharide rich fractions viz. hot water extracted polysaccharide (HWP), cold alkaline extracted polysaccharide (CAP) and hot alkaline extracted polysaccharide (HAP) have been isolated using varying extraction parameters from Macrocybe gigantea, a well-known edible mushroom collected from Gangetic plain of West Bengal and authenticated by DNA barcoding of nrDNA ITS region. Physico-chemical investigation revealed that the fractions were mainly composed of β-configuration in pyranose form of sugars conjugated with small amount of protein. Further analysis presented that polysaccharides were composed of same monosaccharide even in similar order of ratio (D-glucose > D-galactose > D-mannose > D-xylose). However, Dglucose as well as β-glucan were found to be in the highest amount in CAP. The helical structure was determined by Congo red assay which indicated that polysaccharides were in aggregate forms except HWP which consisted of tertiary structure. These diverse structural features may have imparted effect on free radical scavenging activity of polysaccharides where HWP was the most active in all assays. HWP was proved to be a good scavenger of free radicals, strong chelator of ferrous ion and had high reducing power. Thus it can be inferred that HWP may foster further studies for searching active compound which might be used as ingredients of functional foods, nutraceuticals and pharmaceuticals. Moreover, to the best of our knowledge this is the first report on chemical composition and antioxidant activity of different crude polysaccharides from M. gigantea.

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Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms

Cited by 83 publications

References 27 publications

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

Influence of extraction parameters on physico-chemical characters and antioxidant activity of water soluble polysaccharides from Macrocybe gigantea (Massee) Pegler & Lodge

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