Novel Ionic Matrices for Enhanced Ionization of Oligosaccharides/Glycopeptides during MALDI-MS Analysis

匡敏,; Zhang, Ying; 杨芃原,; 陆豪杰,

doi:10.6023/a13030299

Cited by 6 publications

(4 citation statements)

References 21 publications

(23 reference statements)

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“…A novel class of ionic liquid matrices based on THAP and showing superior performance to traditional crystalline matrices has been developed by Kuang et al (). These matrices consist of a combination of pyridine or N , N ‐dimethylaniline (DMA) with 2′,3′,4′‐ or 2′,4′,6‐THAP in the molar ratio of 1:1 and containing ammonium citrate as an additive.…”

Section: Matricesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly- saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 37:353-491, 2018.

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Section: Matricesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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“…This is not surprising as the exact mechanism of ionization during MALDI-MS is not fully understood [6], with the vast majority of new matrices being introduced based on experimental data [31][32][33][34]. It is this fact that prompted us initially to evaluate a novel drug delivery agent ND-Lys as a MALDI matrix.…”

Section: Resultsmentioning

confidence: 99%

The Development of Novel Nanodiamond Based MALDI Matrices for the Analysis of Small Organic Pharmaceuticals

Chitanda

Zhang

Pahl

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. The utility of novel functionalized nanodiamonds (NDs) as matrices for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) is described herein. MALDI-MS analysis of small organic compounds (<1000 Da) is typically complex because of interferences from numerous cluster ions formed when using conventional matrices. To expand the use of MALDI for the analysis of small molecules, novel matrices were designed by covalently linking conventional matrices (or a lysine moiety) to detonated NDs. Four new functionalized NDs were evaluated for their ionization capabilities using five pharmaceuticals with varying molecular structures. Two ND matrices were able to ionize all tested pharmaceuticals in the negative ion mode, producing the deprotonated ions [M -H] -. Ion intensity for target analytes was generally strong with enhanced signal-to-noise ratios compared with conventional matrices. The negative ion mode is of great importance for biological samples as interference from endogenous compounds is inherently minimized in the negative ion mode. Since the molecular structures of the tested pharmaceuticals did not suggest that negative ion mode would be preferable, this result magnifies the importance of these findings. On the other hand, conventional matrices primarily facilitated the ionization as expected in the positive ion mode, producing either the protonated molecules [M + H] + or cationic adducts (typically producing complex spectra with numerous adduct peaks). The data presented in this study suggests that these matrices may offer advantages for the analysis of low molecular weight pharmaceuticals/metabolites.

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“…3, "cool" ionic liquid matrices: 1,1,3,3-tetramethylguanidium (TMG) salt of p-coumaric acid (G 3 CA), 107) and 3-aminoquinoline/pcoumaric acid (3-AQ/CA) 108,109) were developed by Tanaka and coworkers to yield a less locally heated ionization environment for enhancing the sensitivity and suppressing the losses of sialic acid moieties. Applying a similar concept, THAP-based ionic liquid matrices 110) were also reported for automated identi cation and quantitative analysis of native oligosaccharides. Witt et al utilized ice as an extremely so matrix to prevent carbohydrates from decomposition and enhance the capability of MALDI mass spectrometry for the structural characterization of glycans.…”

Section: Mechanistic Studies Of Carbohydrate Ionization In Maldimentioning

confidence: 99%

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Mechanistic Studies and Methods for Improving the Structural Identification of Carbohydrates

Lai

Wang

2017

Mass Spectrometry

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Although matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is one of the most widely used so ionization methods for biomolecules, the lack of detailed understanding of ionization mechanisms restricts its application in the analysis of carbohydrates. Structural identi cation of carbohydrates achieved by MALDI mass spectrometry helps us to gain insights into biological functions and pathogenesis of disease. In this review, we highlight mechanistic details of MALDI, including both ionization and desorption. Strategies to improve the ion yield of carbohydrates are also reviewed. Furthermore, commonly used fragmentation methods to identify the structure are discussed.

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Novel Ionic Matrices for Enhanced Ionization of Oligosaccharides/Glycopeptides during MALDI-MS Analysis

Cited by 6 publications

References 21 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

The Development of Novel Nanodiamond Based MALDI Matrices for the Analysis of Small Organic Pharmaceuticals

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Mechanistic Studies and Methods for Improving the Structural Identification of Carbohydrates

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