Novel Insights on the Regulation of B Cell Functionality by Members of the Tumor Necrosis Factor Superfamily in Jawed Fish

Tafalla, Carolina; Granja, Aitor G.

doi:10.3389/fimmu.2018.01285

Cited by 31 publications

(13 citation statements)

References 179 publications

(279 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The univariate testing of single factors found only a significant association of EBV + patients with higher levels of BAFF. BAFF is known as a key regulator of B cell homeostasis [31][32][33] , and its effects on expansion of EBV-infected B cell could be confirmed in our in vitro experiment. Importantly, no upregulation of the markers for PTLD development including IL-6, IL-10 and TNFα was observed [15][16][17][18] .…”

Section: Discussionsupporting

confidence: 66%

The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation

Bajda

Blázquez-Navarro

Samans³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Epstein-Barr virus (EBV) reactivation can lead to serious complications in kidney transplant patients, including post-transplant lymphoproliferative disorder (PTLD). Here, we have assessed the impact of EBV on B cell homeostasis at cellular and humoral level. In a multicenter study monitoring 540 kidney transplant patients during the first post-transplant year, EBV reactivation was detected in 109 patients. Thirteen soluble factors and B cell counts were analyzed in an EBV+ sub-cohort (N = 54) before, at peak and after EBV clearance and compared to a control group (N = 50). The B cell activating factor (BAFF) was significantly elevated among EBV+ patients. No additional soluble factors were associated with EBV. Importantly, in vitro experiments confirmed the proliferative effect of BAFF on EBV-infected B cells, simultaneously promoting EBV production. In contrast, elevated levels of BAFF in EBV+ patients did not lead to B cell expansion in vivo. Moreover, diminished positive inter-correlations of soluble factors and alterations of the bi-directional interplay between B cell and soluble factors were observed in EBV+ patients at peak and after clearance. Our data suggest that such alterations may counteract the proliferative effect of BAFF, preventing B cell expansion. The role of these alterations in lymphoma development should be analyzed in future studies.

show abstract

Section: Discussionsupporting

confidence: 66%

The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation

Bajda

Blázquez-Navarro

Samans³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The role of various genes in insulin resistance has been investigated among which TNF-α is prominent. Recent reports suggest that TNF-α disrupts insulin signaling pathway through the reduced kinase activity of insulin receptor [28] . The highest expression rate of IL-1β gene was observed in the control and glucose-containing control groups, and there was a significant difference with the other groups (p < 0.05).…”

Section: Gene Factorsmentioning

confidence: 99%

Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Mohammadi

Manouchehri

Changizi

et al. 2020

J Diabetes Metab Disord

View full text Add to dashboard Cite

Objective In this study, zebrafish was used as a biological model to induce type 2 diabetes mellitus through glucose. Then, the effect of metformin and silibinin combination was examined on elevated blood glucose, intestinal tissues, liver enzymes, and TNF-α, IFN-γ, INL1β genes as inflammation marker genes. Methods The liver enzymes (AST, ALT, and ALP) derived from fish viscera homogenate supernatants were assayed in an auto-analyzer. The expression of target genes was quantified on RNA extracted from the tails by an in-house RT-PCR method, with fine intestine tissue staining performed by hematoxylin and eosin protocol (H&E). Result In the glucose-free treatments, metformin and silymarin decreased the levels of AST, ALT, and ALP enzymes in the blood. The combination of these two drugs had also a significant role in reducing glucose levels. The body weight increased significantly in the control group which was affected by glucose concentration, with the lowest body weight gain observed in the metformin group. The expression of INL-1β gene was significantly enhanced in the control group and the highest IFN-γ expression was observed in both control groups with glucose (G + CTRL) and without glucose (G-CTRL) (p < 0.05). The lowest level of TNF-α gene expression was observed in the control + glucose group (G + CTRL) (p < 0.05). Diabetic state causes weak absorption whereby the fish body demands to increase absorption level by enhancing the amount of acidic goblet cells thereby acidifying the environment in the gastric tracts. Conclusion Collectively, this study indicated that treatment with metformin and Silibinin could improve metabolic-mediated performances by reducing the expression of inflammatory genes and blood glucose, modulating liver enzymes, and ameliorating the intestinal inflammation in type 2 diabetic zebrafish model.

show abstract

“…It has been extensively described that members of the TNF superfamily of ligands (TNFSF) and receptors (TNFRSF) play key roles on the homeostasis and activation of B cells both in mammals (46) and fish (47). Among TNFSF, the transcription levels of tnfsf12 (TWEAK) and tnfsf13b (BAFF) were significantly downregulated upon TNP-LPS stimulation but not in response to TNP-KLH ( Figure 5).…”

Section: Analysis Of Differentially Expressed Immune Genes In Isolatementioning

confidence: 98%

Rainbow Trout IgM+ B Cells Preferentially Respond to Thymus-Independent Antigens but Are Activated by CD40L

Granja¹,

Perdiguero²,

Martín-Martín³

et al. 2019

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

In the absence of class switch recombination and germinal centers, the mechanisms through which B cells from teleost fish mount extrafollicular immunoglobulin M (IgM) memory responses remains mostly unexplored. In this report, we demonstrate that teleost IgM + B cells respond to CD40L, a thymus-dependent activation signal, similarly to mammalian B2 cells. However, when stimulated with different types of antigens, fish IgM + B cells only reach a general activation state in response to antigens cataloged as thymus-independent 1 (TI-1) in mammals, as established through both functional assays and RNA sequencing. Interestingly, fish IgM + B cells remained completely unresponsive to TI-2 antigens, suggesting that the engagement of innate receptors provided by TI-1 antigens is required for the activation of teleost B cells. Finally, a synergy between CD40L and TI-1 antigens was also demonstrated, further supporting that there is no clear dichotomy between thymus-dependent and TI responses in teleost fish.

show abstract

Novel Insights on the Regulation of B Cell Functionality by Members of the Tumor Necrosis Factor Superfamily in Jawed Fish

Cited by 31 publications

References 179 publications

The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation

The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation

Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Rainbow Trout IgM+ B Cells Preferentially Respond to Thymus-Independent Antigens but Are Activated by CD40L

Contact Info

Product

Resources

About