“…As a consequence, in order to overexpress hCA IX and XII isoforms in MCF7 cells, before cotreaments of doxo 2.5 μM and compounds 9 , 14 , 16 , and 20 , a hypoxic condition was reproduced by administering cobalt(II) chloride hexahydrate (CoCl 2 ) at 100 μM for 48 h, chemically inducing HIF-1α, as already reported elsewhere. 56 Cotreatments of doxo 2.5 μM + compounds 9 , 14 , 16 , and 20 were kept up to 72 h; next, the metabolic activity was measured by the MTT test. The latter reveals that for both samples exposed to doxo 2.5 μM alone and to cotreatments of doxo 2.5 μM with compounds 9 , 14 , 16 , and 20 at 50 μM, a statistically significant reduction in cell metabolic activity is recorded with respect to cells exposed to DMSO+CoCl 2 , assumed as control ( Figure 8 A).…”