Novel insights into the taxonomic diversity and molecular mechanisms of bacterial Mn(<scp>III</scp>) reduction

Szeinbaum, Nadia; Nunn, Brook L.; Cavazos, Amanda R.; Crowe, Sean A.; Stewart, Frank J.; DiChristina, Thomas J.; Reinhard, Christopher T.; Glass, Jennifer B.

doi:10.1111/1758-2229.12867

Cited by 4 publications

(3 citation statements)

References 60 publications

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“…Sediment had been diluted over 1000‐fold by the time DNA was extracted for sequencing. Details on metagenomes from 395‐day anoxic enrichments of Lake Matano sediment incubated with Mn(III) pyrophosphate are reported in a separate publication (Szeinbaum et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

Bray

Padilla

et al. 2019

Environ Microbiol Rep

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Electroactive type IV pili, or e-pili, are used by some microbial species for extracellular electron transfer. Recent studies suggest that e-pili may be more phylogenetically and structurally diverse than previously assumed. Here, we used updated aromatic density thresholds (≥9.8% aromatic amino acids, ≤22-aa aromatic gaps and aromatic amino acids at residues 1, 24, 27, 50 and/or 51, and 32 and/or 57) to search for putative e-pilin genes in metagenomes from diverse ecosystems with active microbial metal cycling. Environmental putative e-pilins were diverse in length and phylogeny, and included truncated e-pilins in Geobacter spp., as well as longer putative e-pilins in Fe(II)oxidizing Betaproteobacteria and Zetaproteobacteria.

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Section: Methodsmentioning

confidence: 97%

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

Bray

Padilla

et al. 2019

Environ Microbiol Rep

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“…However, in the natural environment with a complex microbial community and harsh conditions, such as aqueous, edaphic, and sedimentary environments, the most widespread electron acceptors is the iron-manganese mineral. , The slightly soluble Fe(III) citrate was used as the terminal electron acceptor in the anaerobic sludge mixed bacteria to test the general applicability in practice. Microorganisms were observed to connect with Fe(III) citrate via microbial nanowires.…”

Section: Results and Discussionmentioning

confidence: 99%

In Situ Enhanced Yields of Microbial Nanowires: The Key Role of Environmental Stress

Song

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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The conductive microbial nanowires of Geobacter sulfurreducens serve as a model for long-range extracellular electron transfer (EET), which is considered a revolutionary "green" nanomaterial in the fields of bioelectronics, renewable energy, and bioremediation. However, there is no efficient pathway to induce microorganisms to express a large amount of microbial nanowires. Here, several strategies have been used to successfully induce the expression of microbial nanowires. Microbial nanowire expression was closely related to the concentration of electron acceptors. The microbial nanowire was around 17.02 μm in length, more than 3 times compared to its own length. The graphite electrode was used as an alternative electron acceptor by G. sulfurreducens, which obtained a fast start-up time of 44 h in microbial fuel cells (MFCs). Meanwhile, Fe(III) citrate-coated sugarcane carbon and biochar were prepared to test the applicability of these strategies in the actual microbial community. The unsatisfied EET efficiency between c-type cytochrome and extracellular insoluble electron receptors promoted the expression of microbial nanowires. Hence, microbial nanowires were proposed to be an effective survival strategy for G. sulfurreducens to cope with various environmental stresses. Based on this top-down strategy of artificially constructed microbial environmental stress, this study is of great significance for exploring more efficient methods to induce microbial nanowires expression.

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“…Although this process has been well-studied, e.g., ( Nealson et al, 1988 ; Tebo et al, 2004 ; Hansel, 2017 ), it was only recently shown that a microorganism can catalyze Mn 2+ oxidation to gain energy ( Yu and Leadbetter, 2020 ). It has also been demonstrated that microorganisms can reduce aqueous ligand-bound Mn(III) ( Kostka et al, 1995 ; Szeinbaum et al, 2014 , 2017 , 2020 ) and solid-phase Mn(III), in the form of manganite (MnOOH) ( Larsen et al, 1998 ; Fredrickson et al, 2002 ), to provide energy for microorganisms.…”

Section: Introductionmentioning

confidence: 99%

The Energetic Potential for Undiscovered Manganese Metabolisms in Nature

2021

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Microorganisms are found in nearly every surface and near-surface environment, where they gain energy by catalyzing reactions among a wide variety of chemical compounds. The discovery of new catabolic strategies and microbial habitats can therefore be guided by determining which redox reactions can supply energy under environmentally-relevant conditions. In this study, we have explored the thermodynamic potential of redox reactions involving manganese, one of the most abundant transition metals in the Earth’s crust. In particular, we have assessed the Gibbs energies of comproportionation and disproportionation reactions involving Mn2+ and several Mn-bearing oxide and oxyhydroxide minerals containing Mn in the +II, +III, and +IV oxidation states as a function of temperature (0–100°C) and pH (1–13). In addition, we also calculated the energetic potential of Mn2+ oxidation coupled to O2, NO2–, NO3–, and FeOOH. Results show that these reactions—none of which, except O2 + Mn2+, are known catabolisms—can provide energy to microorganisms, particularly at higher pH values and temperatures. Comproportionation between Mn2+ and pyrolusite, for example, can yield 10 s of kJ (mol Mn)–1. Disproportionation of Mn3+ can yield more than 100 kJ (mol Mn)–1 at conditions relevant to natural settings such as sediments, ferromanganese nodules and crusts, bioreactors and suboxic portions of the water column. Of the Mn2+ oxidation reactions, the one with nitrite as the electron acceptor is most energy yielding under most combinations of pH and temperature. We posit that several Mn redox reactions represent heretofore unknown microbial metabolisms.

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Novel insights into the taxonomic diversity and molecular mechanisms of bacterial Mn(III) reduction

Cited by 4 publications

References 60 publications

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes

In Situ Enhanced Yields of Microbial Nanowires: The Key Role of Environmental Stress

The Energetic Potential for Undiscovered Manganese Metabolisms in Nature

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