Novel insights into the genetic and epigenetic paternal contribution to the human embryo

Kumar, Manoj; Kumar, K. Ashok; Jain, Shalu; Hassan, Tarannum; Dada, Rima

doi:10.6061/clinics/2013(sup01)02

Cited by 105 publications

(81 citation statements)

References 126 publications

(133 reference statements)

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“…One of the critical factors that is known to play an important role in pregnancy and subsequent embryo development is sperm DNA integrity [Kumar et al 2013;Menezo 2006;Puscheck and Jeyendran 2007;Sakkas and Alvarez 2010;Simon et al 2014]. The deleterious effect of fragmented paternal DNA on embryonic viability and progression of pregnancy has been experimentally shown in mouse models [Ahmadi and Ng 1999] and similar results were also observed in human patients who opt for assisted reproductive technologies such as IVF and ICSI [Lin et al 2008;Muriel et al 2006;Robinson et al 2012;Tandara et al 2014;Tesarik et al 2004;Wdowiak et al 2015;Zhao et al 2014;Zini et al 2008].…”

Section: Introductionsupporting

confidence: 64%

“…Thus far only the histones and protamines have been validated to be a part of non-genomic paternal contribution to embryo [Brunner 2014;Jenkins and Carrell 2011;Kumar et al 2013;Puri et al 2010;Schagdarsurengin et al 2012;van de Werken et al 2014;van der Heijden et al 2008;Ward 2010]. However besides these, a portion of the nuclear matrix proteins and perinuclear theca proteins are also inherited by the newly fertilized embryo and can be implicated to have certain epigenetic significance [Yamauchi et al 2011].…”

Section: Introductionmentioning

confidence: 99%

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Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Mohanty

Swain

Goswami

et al. 2016

Systems Biology in Reproductive Medicine

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Contribution from a defective paternal genome has been attributed to be an important cause for spontaneous recurrent pregnancy loss (RPL). Increased oxidative stress results in decreased detoxification and is a cause for damage to chromatin, proteins, and membrane lipids. The present study aimed to explore if there is a significant relationship between retained histones due to defective packaging of DNA in spermatozoa and oxidative stress. RPL patients (n=16) with a history of ≥2 embryo losses before the 20th week of gestation and no female factor abnormality, and fertile healthy volunteers (n=20) as controls were included in the study. A significant difference in the levels of protein carbonylation and lipid peroxidation together with an increased retention of histones in the experimental groups was noticed. Histone carrying sites for oxidative modification such as arginine and lysine might be responsible for disturbing the paternal epigenomic control during early stages of embryonic differentiation leading to abortion. ARTICLE HISTORY

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Section: Introductionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Mohanty

Swain

Goswami

et al. 2016

Systems Biology in Reproductive Medicine

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“…These factors appear to influence the survival of the embryo post-fertilisation through participation in various molecular functions, such as signal transduction, cell proliferation and transcriptional proliferation (Krawetz 2005;Yamauchi et al 2011;Kumar et al 2013).…”

Section: Mechanisms By Which Heat-stressed Spermatozoa Can Affect Blamentioning

confidence: 99%

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Peña

Gummow

Parker³

et al. 2017

Anim. Prod. Sci.

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Abstract.Temperature is a crucial factor in mammalian spermatogenesis. The scrotum, pampiniform plexus, and cremaster and dartos muscles in mammals are specific adaptations to ensure sperm production in a regulated environment 4À6 C below internal body temperature. However, the limited endogenous antioxidant systems inherent in mammalian spermatozoa compounded by the loss of cytosolic repair mechanisms during spermatogenesis, make the DNA in these cells particularly vulnerable to oxidative damage. Boar sperm is likely to be more susceptible to the effects of heat stress and thus oxidative damage due to the relatively high unsaturated fatty acids in the plasma membrane, low antioxidant capacity in boar seminal plasma, and the boar's non-pendulous scrotum. Heat stress has a significant negative impact on reproductive performance in piggeries, which manifests as summer infertility and results in productivity losses that amount to millions of dollars. This problem is particularly prevalent in tropical and subtropical regions where ambient temperatures rise beyond the animal's zone of thermal comfort. Based on preliminary studies in the pig and other species, this article discusses whether heat stress could induce sufficient DNA damage in boar sperm to significantly contribute to the high rates of embryo loss and pregnancy failure observed in the sow during summer infertility. Heat stress-induced damage to sperm DNA can lead to disrupted expression of key developmental genes essential for the differentiation of early cell lineages, such as the trophectoderm, and can distort the timely formation of the blastocyst; resulting in a failure of implantation and ultimately pregnancy loss. Confirming such a link would prompt greater emphasis on boar management and strategies to mitigate summer infertility during periods of heat stress.

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“…So far, the examination of the male factor and its influence on the results is carried out mainly with seminal morphological parameters. We know that DNA integrity is negatively correlated with infertility and has a direct impact on the quality of the embryo and its development [5]. Damage to DNA is related mainly to oxidative stress (OS).…”

Section: Discussionmentioning

confidence: 99%

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

Sánchez¹

2017

OGIJ

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The semen volume is made up by the accessory glands (90%) and germinal cells (10%) (RB Mayer, 2015). Seminal plasma has enabling factors for sperm microenvironment (Aida Pujol, 2016), allowing among many other processes, sperm maturation (Yu-Wen Kuo, 2016).Objective: To analyze the correlation of seminal volume parameters with the outcome of fertilized oocytes. Material and methods:It is a prospective observational study, including 100 couples undergoing in vitro fertilization. The seminal sample was obtained by ejaculation and analyzed with WHO's 2010 criteria (Andrology laboratory procedures manual, 2010). Data encompassed seminal volumes and fertilized oocytes. Statistical analysis was made with Kolmogorov-Smirnov normalization, determining correlation of Pearson and significance of p ≤0.05. Results:The following parameters were registered: Seminal volume: 1.5-7.6 (average 2.7 ml), number of fertilized MII oocytes: 1-33 (average 10 oocytes). Pearson correlation of 0.737 (p= 0.002). By dividing groups of fertilized oocytes and seminal volumes by standard Kolmogorov we obtained significance with the number of fertilized eggs with 4.8 and 14 oocytes (p=0.001), and we also obtained significance with the seminal volume, ranging from 2 to 3.2ml (p=0.003).Discussion: Our study shows that it exists a prognostic relationship between the seminal volume and the number of fertilized eggs in an in vitro fertilization. A seminal volume ranging from 2 to 3.2ml has a better chance to produce adequate fertilization. Volumes lower than this values may reflect inadequate sperm formation and regulation and volumes greater may reflect other inflammatory conditions, such as infection (Jared M Bienek, 2016). Conclusion:Seminal volume reflects a healthy testicular function, producing spermatozoa capable of achieving good fertilization. Alterations on the seminal volume indicate inadequate conditions for the correct formation of spermatozoa, or increased inflammation molecules that damage the microenvironment.

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Novel insights into the genetic and epigenetic paternal contribution to the human embryo

Cited by 105 publications

References 126 publications

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

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