Novel Insights into miRNA Regulation of Storage Protein Biosynthesis during Wheat Caryopsis Development under Drought Stress

Chen, Xinyu; Yang, Yang; Ran, Liping; Dong, Zhaodi; Zhang, Erjin; Yu, Xurun

doi:10.3389/fpls.2017.01707

Cited by 22 publications

(13 citation statements)

References 60 publications

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“…The accumulation of stress proteins in developing wheat grains can significantly increase under water-deficit stress, which would also affect the protein-protein interaction network 46,47 . The stress-responsive miRNA-guided regulation of storage protein biosynthesis could play a key role here 29,48 . The accumulation of stress proteins, storage metabolites and compounds with antioxidant properties (such as phenolics) in maternal plants may potentially facilitate more growth-effective metabolic processes and a swift response in progeny plants upon recurring stress conditions 8,45 .…”

Section: Discussionmentioning

confidence: 99%

Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

Liu

Able

2021

Sci Rep

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Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on parental plants during reproduction may have transgenerational effects on progeny. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in first generation progeny. Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter-generational stress. Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained at the molecular level indicate that key miRNA-mRNA modules are candidates for transgenerational stress improvement.

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Section: Discussionmentioning

confidence: 99%

Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

Liu

Able

2021

Sci Rep

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“…However, the response mechanism that modifies protein accumulation under water deficit conditions is still unclear. Recently, a putative mechanism underlying the increased accumulation of storage proteins in wheat endosperm under water deficit was provided by Chen et al [ 63 ]. They identified four differentially expressed miRNAs induced by drought stress that may affect the development of protein bodies in caryopsis by regulating the expression levels of target genes involved in protein biosynthesis pathways.…”

Section: Discussionmentioning

confidence: 99%

Identification of Genomic Regions Contributing to Protein Accumulation in Wheat under Well-Watered and Water Deficit Growth Conditions

Elbasyoni

Morsy

Ramamurthy

et al. 2018

Plants

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Sustaining wheat production under low-input conditions through development and identifying genotypes with enhanced nutritional quality are two current concerns of wheat breeders. Wheat grain total protein content, to no small extent, determines the economic and nutritive value of wheat. Therefore, the objectives of this study are to identify accessions with high and low grain protein content (GPC) under well-watered and water-deficit growth conditions and to locate genomic regions that contribute to GPC accumulation. Spring wheat grains obtained from 2111 accessions that were grown under well-watered and water-deficit conditions were assessed for GPC using near-infrared spectroscopy (NIR). Results indicated significant influences of moisture, genotype, and genotype × environment interaction on the GPC accumulation. Furthermore, genotypes exhibited a wide range of variation for GPC, indicating the presence of high levels of genetic variability among the studied accessions. Around 366 (166 with high GPC and 200 with low GPC) wheat genotypes performed relatively the same across environments, which implies that GPC accumulation in these genotypes was less responsive to water deficit. Genome-wide association mapping results indicated that seven single nucleotide polymorphism (SNPs) were linked with GPC under well-watered growth conditions, while another six SNPs were linked with GPC under water-deficit conditions only. Moreover, 10 SNPs were linked with GPC under both well-watered and water-deficit conditions. These results emphasize the importance of using diverse, worldwide germplasm to dissect the genetic architecture of GPC in wheat and identify accessions that might be potential parents for high GPC in wheat breeding programs.

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“…In wheat, about a dozen studies have been performed on miRNA identification using sRNA sequencing (sRNA-Seq) (Yao et al, 2007; Wei et al, 2009; Li et al, 2013; Meng et al, 2013; De Paola et al, 2016; Fileccia et al, 2017; Wang et al, 2018). miRNAs responding to diverse stresses in wheat have also been reported (Xin et al, 2010; Zhou et al, 2015; Akdogan et al, 2016; Chen et al, 2017; Zuluaga et al, 2017; Han et al, 2018). miRNAs related to the development and filling of wheat seeds have been studied (Meng et al, 2013; Han et al, 2014; Li et al, 2015; Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Wei

Wang

et al. 2019

Front. Genet.

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Cold acclimation and vegetative/reproductive transition are two important evolutionary adaptive mechanisms for winter wheat surviving the freezing temperature in winter and successful seeds setting in the next year. MicroRNA (miRNA) is a class of regulatory small RNAs (sRNAs), which plays critical roles in the growth and development of plants. However, the regulation mechanism of miRNAs during cold acclimation and vegetative/reproductive transition of winter wheat is not much understood. In this study, four sRNA libraries from leaves of winter wheat grown in the field at the three-leaf stage, winter dormancy stage, spring green-up stage, and jointing stage were analyzed to identify known and novel miRNAs and to understand their potential roles in the growth and development of winter wheat. We examined miRNA expression using a high-throughput sequencing technique. A total of 373 known, 55 novel, and 27 putative novel miRNAs were identified. Ninety-one miRNAs were found to be differentially expressed at the four stages. Among them, the expression of six known and eight novel miRNAs was significantly suppressed at the winter dormancy stage, whereas the expression levels of seven known and eight novel miRNAs were induced at this stage; three known miRNAs and three novel miRNAs were significantly induced at the spring green-up stage; six known miRNAs were induced at the spring green-up stage and reached the highest expression level at the jointing stage; and 20 known miRNAs and 10 novel miRNAs were significantly induced at the jointing stage. Expression of a number of representative differentially expressed miRNAs was verified using quantitative real-time polymerase chain reaction (qRT-PCR). Potential target genes for known and novel miRNAs were predicted. Moreover, six novel target genes for four Pooideae species-specific miRNAs and two novel miRNAs were verified using the RNA ligase-mediated 5′-rapid amplification of cDNA ends (RLM-5’RACE) technique. These results indicate that miRNAs are key non-coding regulatory factors modulating the growth and development of wheat. Our study provides valuable information for in-depth understanding of the regulatory mechanism of miRNAs in cold acclimation and vegetative/reproductive transition of winter wheat grown in the field.

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Novel Insights into miRNA Regulation of Storage Protein Biosynthesis during Wheat Caryopsis Development under Drought Stress

Cited by 22 publications

References 60 publications

Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

Identification of Genomic Regions Contributing to Protein Accumulation in Wheat under Well-Watered and Water Deficit Growth Conditions

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

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