Novel insight into the underlying dysregulation mechanisms of immune cell-to-cell communication by analyzing multitissue single-cell atlas of two COVID-19 patients

Qin, Si; Yao, Xiaohong; Li, Weiwei; Wang, Canbiao; Xu, Weijun; Zhen-hua, Gan; Yang, Yang; Zhong, Aihua; Wang, Bin; He, Zhicheng; Wu, Jian; Wu, Qiuyue; Jiang, Wenhua; Han, Ying; Wang, Fan; Wang, Zhihua; Ke, Yuehua; Zhao, Jun; Gao, Junyin; Qu, Liang; Park, Jin; Guan, Miao; Xia, Xinyi; Bian, Xiu‐Wu

doi:10.1038/s41419-023-05814-z

Cited by 7 publications

(4 citation statements)

References 64 publications

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“…FPR1 was found to induce blood neutrophil activation in COVID-19 patients, triggering neutrophil-mediated inflammation and end-organ damage [43]. It has also been found that T cells and myeloid cells from COVID-19 patients may trigger an inflammatory storm in the lungs through intercellular communication via the ANXA1/FPR1 receptor-ligand signaling pathway [44] The study by Lee H et al [45] also concluded that FPR1 is a suitable target for appropriate assessment of COVID-19 severity and provision of therapeutic agents. FCGR2A is a member of the family of genes encoding immunoglobulin Fc receptors present on the surface of many immuneresponsive cells.…”

Section: Discussionmentioning

confidence: 99%

Immune Characteristic Genes and Neutrophil Immune Transformation Studies in Severe COVID-19

Zhou,

Zeng,

Liao

et al. 2024

Microorganisms

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As a disease causing a global pandemic, the progression of symptoms to severe disease in patients with COVID-19 often has adverse outcomes, but research on the immunopathology of COVID-19 severe disease remains limited. In this study, we used mRNA-seq data from the peripheral blood of COVID-19 patients to identify six COVID-19 severe immune characteristic genes (FPR1, FCGR2A, TLR4, S100A12, CXCL1, and L TF), and found neutrophils to be the critical immune cells in COVID-19 severe disease. Subsequently, using scRNA-seq data from bronchoalveolar lavage fluid from COVID-19 patients, neutrophil subtypes highly expressing the S100A family were found to be located at the end of cellular differentiation and tended to release neutrophil extracellular traps. Finally, it was also found that alveolar macrophages, macrophages, and monocytes with a high expression of COVID-19 severe disease immune characteristic genes may influence neutrophils through intercellular ligand–receptor pairs to promote neutrophil extracellular trap release. This study provides immune characteristic genes, critical immune pathways, and immune cells in COVID-19 severe disease, explores intracellular immune transitions of critical immune cells and pit-induced intercellular communication of immune transitions, and provides new biomarkers and potential drug targets for the treatment of patients with COVID-19 severe disease.

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Section: Discussionmentioning

confidence: 99%

Immune Characteristic Genes and Neutrophil Immune Transformation Studies in Severe COVID-19

Zhou,

Zeng,

Liao

et al. 2024

Microorganisms

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“…Mucins belong to the first defense against pathogens in the airways and are essential for mucociliary correction; high expression of MUC5B may be related to worse prognosis of SARS-CoV-2 infection [ 8 , 14 , 17 ]. The rs35705950 SNP present in this gene is described as an important variant associated with hospitalization and idiopathic pulmonary fibrosis [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation between Genomic Variants and Worldwide COVID-19 Epidemiology

Costa,

Albarello Gellen,

Fernandes

et al. 2024

JPM

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COVID-19 is a systemic disease caused by the etiologic agent SARS-CoV-2, first reported in Hubei Province in Wuhan, China, in late 2019. The SARS-CoV-2 virus has evolved over time with distinct transmissibility subvariants from ancestral lineages. The clinical manifestations of the disease vary according to their severity and can range from asymptomatic to severe. Due to the rapid evolution to a pandemic, epidemiological studies have become essential to understand and effectively combat COVID-19, as the incidence and mortality of this disease vary between territories and populations. This study correlated epidemiological data on the incidence and mortality of COVID-19 with frequencies of important SNPs in GWAS studies associated with the susceptibility and mortality of this disease in different populations. Our results indicated significant correlations for 11 genetic variants (rs117169628, rs2547438, rs2271616, rs12610495, rs12046291, rs35705950, rs2176724, rs10774671, rs1073165, rs4804803 and rs7528026). Of these 11 variants, 7 (rs12046291, rs117169628, rs1073165, rs2547438, rs2271616, rs12610495 and rs35705950) were positively correlated with the incidence rate, these variants were more frequent in EUR populations, suggesting that this population is more susceptible to COVID-19. The rs2176724 variant was inversely related to incidence rates; therefore, the higher the frequency of the allele is, the lower the incidence rate. This variant was more frequent in the AFR population, which suggests a protective factor against SARS-CoV-2 infection in this population. The variants rs10774671, rs4804803, and rs7528026 showed a significant relationship with mortality rates. SNPs rs10774671 and rs4804803 were inversely related to mortality rates and are more frequently present in the AFR population. The rs7528026 variant, which is more frequent in the AMR population, was positively related to mortality rates. The study has the potential to identify and correlate the genetic profile with epidemiological data, identify populations that are more susceptible to severe forms of COVID-19, and relate them to incidence and mortality.

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“…By exposing human HEK293T cells to the SARS-CoV-2 spike gene plasmid, the synthesis of spike glycoprotein is induced within the cell, which releases large amounts of EVs containing the spike glycoprotein along with specific miRNAs [89,95]. Furthermore, long-distance cell-to-cell communication mediated by EVs may be a mechanism by which neurological symptoms manifest in severe cases of COVID-19 [96,97]. Through inhibition of ubiquitin-specific peptidase 33 (USP33) and IRF9, two key proteins that control this pathway, two miRNAs, miR-148a and miR-590, specifically inhibit type I interferon signaling [98].…”

Section: Vaccination With Nanoparticles (Lnp) and Extracellular Vesic...mentioning

confidence: 99%

Understanding the Molecular Actions of Spike Glycoprotein in SARS-CoV-2 and Issues of a Novel Therapeutic Strategy for the COVID-19 Vaccine

Matsuzaka,

Yashiro

2024

BioMedInformatics

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In vaccine development, many use the spike protein (S protein), which has multiple “spike-like” structures protruding from the spherical structure of the coronavirus, as an antigen. However, there are concerns about its effectiveness and toxicity. When S protein is used in a vaccine, its ability to attack viruses may be weak, and its effectiveness in eliciting immunity will only last for a short period of time. Moreover, it may cause “antibody-dependent immune enhancement”, which can enhance infections. In addition, the three-dimensional (3D) structure of epitopes is essential for functional analysis and structure-based vaccine design. Additionally, during viral infection, large amounts of extracellular vesicles (EVs) are secreted from infected cells, which function as a communication network between cells and coordinate the response to infection. Under conditions where SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) molecular vaccination produces overwhelming SARS-CoV-2 spike glycoprotein, a significant proportion of the overproduced intracellular spike glycoprotein is transported via EVs. Therefore, it will be important to understand the infection mechanisms of SARA-CoV-2 via EV-dependent and EV-independent uptake into cells and to model the infection processes based on 3D structural features at interaction sites.

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Novel insight into the underlying dysregulation mechanisms of immune cell-to-cell communication by analyzing multitissue single-cell atlas of two COVID-19 patients

Cited by 7 publications

References 64 publications

Immune Characteristic Genes and Neutrophil Immune Transformation Studies in Severe COVID-19

Immune Characteristic Genes and Neutrophil Immune Transformation Studies in Severe COVID-19

Correlation between Genomic Variants and Worldwide COVID-19 Epidemiology

Understanding the Molecular Actions of Spike Glycoprotein in SARS-CoV-2 and Issues of a Novel Therapeutic Strategy for the COVID-19 Vaccine

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