Novel insecticide polymer chemistry to reduce the enzymatic digestion of a protein pesticide, trypsin modulating oostatic factor (TMOF)

Shen, Hongyan; Brandt, Alan E.; Witting-Bissinger, B. E.; Gunnoe, T. Brent; Roe, R. Michael

doi:10.1016/j.pestbp.2009.02.004

Cited by 16 publications

(9 citation statements)

References 20 publications

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“…TALOS+ was used to predict protein backbone torsion angles from the NMR chemical shifts [25], [26]. The 2D NOESY spectrum was automatically assigned and an ensemble of structures calculated without manual intervention using the program CYANA [27].…”

Section: Methodsmentioning

confidence: 99%

Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula

et al. 2013

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Many insect pests have developed resistance to existing chemical insecticides and consequently there is much interest in the development of new insecticidal compounds with novel modes of action. Although spiders have deployed insecticidal toxins in their venoms for over 250 million years, there is no evolutionary selection pressure on these toxins to possess oral activity since they are injected into prey and predators via a hypodermic needle-like fang. Thus, it has been assumed that spider-venom peptides are not orally active and are therefore unlikely to be useful insecticides. Contrary to this dogma, we show that it is possible to isolate spider-venom peptides with high levels of oral insecticidal activity by directly screening for per os toxicity. Using this approach, we isolated a 34-residue orally active insecticidal peptide (OAIP-1) from venom of the Australian tarantula Selenotypus plumipes. The oral LD50 for OAIP-1 in the agronomically important cotton bollworm Helicoverpa armigera was 104.2±0.6 pmol/g, which is the highest per os activity reported to date for an insecticidal venom peptide. OAIP-1 is equipotent with synthetic pyrethroids and it acts synergistically with neonicotinoid insecticides. The three-dimensional structure of OAIP-1 determined using NMR spectroscopy revealed that the three disulfide bonds form an inhibitor cystine knot motif; this structural motif provides the peptide with a high level of biological stability that probably contributes to its oral activity. OAIP-1 is likely to be synergized by the gut-lytic activity of the Bacillus thuringiensis Cry toxin (Bt) expressed in insect-resistant transgenic crops, and consequently it might be a good candidate for trait stacking with Bt.

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Section: Methodsmentioning

confidence: 99%

Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula

et al. 2013

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“…A strategy of developing AcI release systems for insecticide effect including hydrophilic polymer conjugated to TMOF (trypsin modulating oostatic factor) increased stability and inhibited metabolism. It enhanced the bioaccumulation insecticide across the insect gut lumen in the hemolymph (Spodoptera frugiperda), inhibiting oocyte maturation and reducing insect reproduction [19,22,56].…”

Section: Physical and Chemical Systems For Agrochemical Deliverymentioning

confidence: 99%

Nanopesticides in Agriculture: Benefits and Challenge in Agricultural Productivity, Toxicological Risks to Human Health and Environment

Chaud

Souto

Zielińska

et al. 2021

Toxics

153

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Nanopesticides are nanostructures with two to three dimensions between 1 to 200 nm, used to carry agrochemical ingredients (AcI). Because of their unique properties, the loading of AcI into nanoparticles offers benefits when compared to free pesticides. However, with the fast development of new engineered nanoparticles for pests’ control, a new type of environmental waste is being produced. This paper describes the nanopesticides sources, the harmful environmental and health effects arising from pesticide exposure. The potential ameliorative impact of nanoparticles on agricultural productivity and ecosystem challenges are extensively discussed. Strategies for controlled release and stimuli-responsive systems for slow, sustained, and targeted AcI and genetic material delivery are reported. Special attention to different nanoparticles source, the environmental behavior of nanopesticides in the crop setting, and the most recent advancements and nanopesticides representative research from experimental results are revised. This review also addresses some issues and concerns in developing, formulating and toxicity pesticide products for environmentally friendly and sustainable agriculture.

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“…TMOF inhibits trypsin biosynthesis and reduces fitness of target insects (Borovsky & Nauen, ). Several methods have been proposed to improve TMOF delivery to hemolymph and insecticidal activity (Shen et al., ; Jeffers et al., ; Kamareddine et al., ). In addition, higher larval mortality was achieved by applying TMOF with other insecticidal proteins, such as toxins of B. thuringiensis (Borovsky et al., ; Misni et al., ; Saiful et al., ) and nucleopolyhedrovirus chitinase A (Fiandra et al., ).…”

Section: Trypsin As a Target Of Pest Controlmentioning

confidence: 99%

Dietary and phylogenetic correlates of digestive trypsin activity in insect pests

Lazarević

Janković-Tomanić

2015

Entomologia Exp Applicata

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Because food proteins are crucial for insect survival, growth, and fecundity, enzymes involved in their digestion have attracted the attention of fundamental entomologists studying the mechanisms and patterns of dietary specialization, and applied entomologists searching for more efficient modes of pest control. Most insects digest proteins using trypsin, an endopeptidase that cleaves polypeptide chains on the carboxyl side of arginine and lysine, two basic amino acids. As the most ancestral proteinase, trypsin is wide-spread in the digestive tract of insects from various orders and with various feeding habits. The present review focuses on biochemical and molecular characteristics, mechanisms of regulation, and adaptive/non-adaptive changes of trypsin activity in response to heterogeneous food environments in a phylogenetic context. Within-and among-species variations in trypsin structure and regulation that contribute to better matching with specific food types are emphasized. We also discuss the relevance of these data for choosing an appropriate strategy for control of insect pests. Pest control strategies to reduce trypsin activity by interfering with substrate binding or trypsin synthesis and secretion have been suggested. Successful application of these procedures requires a multidisciplinary approach and knowledge about digestive physiology of the pest, as well as the structural characteristics of trypsins that determine their interaction with proteinaceous inhibitors and other food compounds, about patterns of developmental changes in trypsin gene expression, adaptive responses of insects to food composition, and various environmental effects on trypsin activity.

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Novel insecticide polymer chemistry to reduce the enzymatic digestion of a protein pesticide, trypsin modulating oostatic factor (TMOF)

Cited by 16 publications

References 20 publications

Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula

Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula

Nanopesticides in Agriculture: Benefits and Challenge in Agricultural Productivity, Toxicological Risks to Human Health and Environment

Dietary and phylogenetic correlates of digestive trypsin activity in insect pests

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