Novel inoculants for an environmentally-friendly crop production

Kozyrovska, Natalia; Kovtunovych, Gennadiy; Gromosova, E. N.; Kuharchuk, P.; Kordyum, V. A.

doi:10.1016/s0166-1116(97)80085-4

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Indeed, including EPS as a matrix component in formulations has been recognized as a novel strategy to protect cells and support plant colonization (Arora and Mishra, ). One of the earliest reports describing this approach concerns the improvement of the shelf life of an inoculant based on Klebsiella oxytoca and Xanthomonas maltophilia , using the EPS mucilan produced by Bacillus mucilaginosus (Kozyrovska et al ., ). More recently, the relevance of EPS was shown by Tewari and Arora (), who triggered EPS production of Pseudomonas aeruginosa by cultivating the bacteria in medium amended with up to 1.6 M NaCl.…”

Section: Desiccation Damage and Protective Strategiesmentioning

confidence: 97%

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Berninger

López

Bejarano

et al. 2017

Microbial Biotechnology

153

106

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SummaryThe application of beneficial, plant‐associated microorganisms is a sustainable approach to improving crop performance in agriculture. However, microbial inoculants are often susceptible to prolonged periods of storage and deleterious environmental factors, which negatively impact their viability and ultimately limit efficacy in the field. This particularly concerns non‐sporulating bacteria. To overcome this challenge, the availability of protective formulations is crucial. Numerous parameters influence the viability of microbial cells, with drying procedures generally being among the most critical ones. Thus, technological advances to attenuate the desiccation stress imposed on living cells are key to successful formulation development. In this review, we discuss the core aspects important to consider when aiming at high cell viability of non‐sporulating bacteria to be applied as microbial inoculants in agriculture. We elaborate the suitability of commonly applied drying methods (freeze‐drying, vacuum‐drying, spray‐drying, fluidized bed‐drying, air‐drying) and potential measures to prevent cell damage from desiccation (externally applied protectants, stress pre‐conditioning, triggering of exopolysaccharide secretion, ‘helper’ strains). Furthermore, we point out methods for assessing bacterial viability, such as colony counting, spectrophotometry, microcalorimetry, flow cytometry and viability qPCR. Choosing appropriate technologies for maintenance of cell viability and evaluation thereof will render formulation development more efficient. This in turn will aid in utilizing the vast potential of promising, plant beneficial bacteria as sustainable alternatives to standard agrochemicals.

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Section: Desiccation Damage and Protective Strategiesmentioning

confidence: 97%

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Berninger

López

Bejarano

et al. 2017

Microbial Biotechnology

153

106

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“…In symbiotically conferred stress tolerance, the endophytic fungi were considered to act as a type of biological trigger that activated the defense systems of a host (Rodriguez and Redman, 2008 ). For example, endophytic fungi that were inoculated to crop plants improved the resistance and yield of the crops (Kozyrovska et al, 1996 ), and such a endophytic-mediated plant resistance to pathogens was more likely the result of direct competition between host plants and pathogens.…”

Section: Beneficial Relationships Conferred By Endophytic Fungi To Homentioning

confidence: 99%

A Friendly Relationship between Endophytic Fungi and Medicinal Plants: A Systematic Review

et al. 2016

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Endophytic fungi or endophytes exist widely inside the healthy tissues of living plants, and are important components of plant micro-ecosystems. Over the long period of evolution, some co-existing endophytes and their host plants have established a special relationship with one and another, which can significantly influence the formation of metabolic products in plants, then affect quality and quantity of crude drugs derived from medicinal plants. This paper will focus on the increasing knowledge of relationships between endophytic fungi and medicinal plants through reviewing of published research data obtained from the last 30 years. The analytical results indicate that the distribution and population structure of endophytes can be considerably affected by factors, such as the genetic background, age, and environmental conditions of their hosts. On the other hand, the endophytic fungi can also confer profound impacts on their host plants by enhancing their growth, increasing their fitness, strengthening their tolerances to abiotic and biotic stresses, and promoting their accumulation of secondary metabolites. All the changes are very important for the production of bioactive components in their hosts. Hence, it is essential to understand such relationships between endophytic fungi and their host medicinal plants. Such knowledge can be well exploited and applied for the production of better and more drugs from medicinal plants.

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“…The use in agriculture of microorganisms that can increase the nutrition of plants and/or reduce the populations of pathogens in the environment can translate into increased crop yields while reducing mineral fertilization rates and the use of chemical plant protection products, and in some cases, completely replacing them (Keel et al 1992;Kozyrovska et al 1996;Canbolat et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of Organic Cultivation on the Occurrence of Beneficial Groups of Microorganisms in the Rhizosphere Soil of Vegetable Crops

Trzciński

Sas-Paszt

Głuszek

et al. 2018

Journal of Horticultural Research

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The article presents the results of research on the occurrence of beneficial groups of microorganisms in the rhizosphere of carrot, parsley and potato plants after the application of: (1) a commercial product EmFarma Plus (Probiotics Polska), (2) a consortium called Skierniewickie Microorganisms (MS) (containing three strains of Klebsiella oxytoca, Pseudomonas fluorescens, Pseudomonas sp.), and (3) Consortium MS together with EmFarma Plus. The study estimated the populations of microscopic fungi and bacteria, including the groups of microorganisms considered to be beneficial, i.e., spore-forming bacteria, fluorescent bacteria of the genus Pseudomonas, diazotrophs and actinomycetes. Applications of Consortium MS and, to a lesser extent, the preparation EmFarma Plus together with Consortium MS resulted in a significant increase in the total population of diazotrophs isolated from the rhizosphere soil of the vegetable species included in the study. There was no significant impact of the application of EmFarma Plus on the population size of the analyzed groups of microorganisms in the rhizosphere soil.

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Novel inoculants for an environmentally-friendly crop production

Cited by 4 publications

References 11 publications

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

A Friendly Relationship between Endophytic Fungi and Medicinal Plants: A Systematic Review

Effect of Organic Cultivation on the Occurrence of Beneficial Groups of Microorganisms in the Rhizosphere Soil of Vegetable Crops

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