Novel infection strategies of <i>Colletotrichum acutatum</i> on ripe blueberry fruit

Wharton, P. S.; Schilder, A. M. C.

doi:10.1111/j.1365-3059.2007.01698.x

Cited by 51 publications

(53 citation statements)

References 29 publications

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“…The symptoms of C. acutatum on susceptible fruit were observed when the mycelium breaks through the fruits' cuticle and produces acervuli (Figure 1d, 3a, 3b). Similar results related to the acervuli sporulation, were reported by Wharton and Schilder (2008) on blueberry cultivars 'Jersey' (susceptible) at 108 hai and 'Elliott' (resistant) at 144 hai. Smith et al, (1999) reported that acervuli sporulation on C. dematium-cowpea stems pathosystem only became visible approximately 80 hai and on leaves brown lesions became visible only 100 hai.…”

Section: Discussionsupporting

confidence: 75%

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Gomes¹,

Bacelar²,

Martins-Lopes³

et al. 2012

JAS

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Colletotrichum acutatum is a serious concern for the Portuguese oliviculture and food industry, due to both olive oil yield and quality decrease. While the organism's phytopathogenic potential has been well documented, the pathogen adhesion and colonization process in olives remain poorly understood. This paper reports experiments conducted on C. acutatum-susceptible, C. acutatum-moderately resistant and C. acutatum-resistant olive fruits during infection process, in two consecutively years, to identify the physical differences related to the host cuticle promoting resistance and susceptibility to C. acutatum. Cuticle thickness, perimeter and area of epidermal cells of 'Galega' (susceptible), 'Cobrançosa' (moderate-resistant) and 'Picual' (resistant) fruits were measured. Here, we also describe the colonization process of olive fruits by C. acutatum. To achieve this goal olive fruits 102 from 'Galega', 'Cobrançosa' and 'Picual' were inoculated in a field trial with an aqueous suspension of C. acutatum (1x10 6 spores ml -1 ) under suitable humidity and temperature conditions. Light, fluorescent and scanning electron microscopy were used to view olive fruit sections. Significant differences were observed among the parameters studied: cuticle thickness, perimeter and area of olive fruit epidermal cells. The C. acutatum-resistant ('Picual') fruits showed highest mean values for each parameter in both years. Pathogen ultrastructures, such as spores, germ tube, and appressoria were clearly observed using different microscopy techniques. Acervuli sporulation was observed only 192 hours after inoculation in C. acutatum-susceptible ('Galega') fruits.

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Section: Discussionsupporting

confidence: 75%

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Gomes¹,

Bacelar²,

Martins-Lopes³

et al. 2012

JAS

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“…Fungal disease in the field is one of the main causes of economic losses in the blueberry sector (Tournas & Katsoudas 2005;Luan et al 2007;Vásquez et al 2007;Wharton & Schilder 2008;Rivera et al 2009). The best control of these microorganisms is by applying good cultural practices and suitable fungicide selection.…”

Section: Introductionmentioning

confidence: 97%

“…The best control of these microorganisms is by applying good cultural practices and suitable fungicide selection. For this reason, strict controls are required to avoid their contamination during preharvesting (flowering and fruit set), harvesting and blueberry processing (Tournas & Katsoudas 2005;Luan et al 2007;Vásquez et al 2007;Wharton & Schilder 2008;Rivera et al 2009;Munitz et al 2013a). Sometimes, unsuitable agricultural practices are used during the application of these active materials.…”

Section: Introductionmentioning

confidence: 97%

Development and validation of an SPME-GC method for a degradation kinetics study of propiconazole I, propiconazole II and tebuconazole in blueberries in Concordia, the main production area of Argentina

Munitz

Medina

Montti

2017

Food Additives & Contaminants: Part A

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An analytical method for the simultaneous determination of propiconazole isomers and tebuconazole residues in blueberries was developed using solid-phase microextraction (SPME) coupled to gas chromatography. Confirmation was performed by gas chromatography-mass spectrometry in selected-ion monitoring mode. The SPME fibre coating selected was CWX-DVB, and the pH was adjusted to 7 with NaOH. The method is selective with adequate precision and high accuracy and sensitivity. Recoveries ranged between 97.4% and 98.9% for all compounds; and detection and quantification limits were respectively 0.21 and 0.49 μg kg for propiconazole I; 0.16 and 0.22 μg kg for propiconazole II; and 0.16 and 0.48 μg kg for tebuconazole. The degradation of these fungicides in blueberries followed first-order rate kinetics. The half-life times for flowering and fruit set applications were respectively 4.0 and 10.3 days for propiconazole I, 4.0 and 11.4 days for propiconazole II, and 3.5 and 12.4 days for tebuconazole.

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“…Studies have shown that colonization strategy adopted by C. acutatum depends on the host (CURRY et al, 2002;DIÉGUEZ-URIBEONDO et al, 2005;SCHILDER, 2008), type of vegetal tissue infected (DIÉGUEZ-URIBEONDO et al, 2005;PERES et al, 2005) or host cultivar (WHARTON; SCHILDER, 2008).…”

Section: S R G Moraes Et Almentioning

confidence: 99%

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

2013

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-Anthracnose, caused by Colletotrichum gloeosporioides, produces brown lesions on guava fruits, causing severe losses on postharvest. In this study, the infection and colonization of guava fruits by C. gloeosporioides has been examined using scanning and transmission electron microscopy. Fruits at the physiologically mature stage were inoculated with a 10 5 conidia/mL spore suspension. Afterward, fruits were incubated at 25 °C in a wet chamber for periods of 6, 12, 24, 48, 96 and 120 h to allow examination of the infection and colonization process. Conidia germination and appressoria formation occurred six hours after inoculation (h.a.i). Penetration occurred directly via penetration pegs from appressoria, which penetrated the host cuticle 48 h.a.i. Notably, the appressoria did not produce an appressorial cone surrounding the penetration pore. Infection vesicles were found in epidermal cells 96 h.a.i. The same fungal structures were found in epidermal and parenchymal cells of the host 120 h.a.i. Colonization strategy of C. gloeosporioides on guava fruit was intracellular hemibiotrophic. Index terms: colonization, penetration, pre-penetration, ultrastructure. HISTOPATOLOGIA DE Colletotrichum gloeosporioides EM FRUTOS DE GOIABEIRA (Psidium guajava L.).RESUMO -A antracnose, causada por Colletotrichum gloeosporioides, é caracterizada por lesões marrons nos frutos de goiabeira, causando severas perdas em pós-colheita. Os processos de infecção e colonização de C. gloeosporioides foram estudados usando microscópio eletrônico de varredura e transmissão. Frutos em plena maturação fisiológica foram inoculados com suspensão de 10 5 conídios/mL. Posteriormente, os frutos foram incubados em câmara úmida a 25 °C, por períodos de molhamento de 6; 12; 24; 48; 96 e 120 h para o estudo do processo de infecção e colonização. Germinação de conídios e formação de apressórios ocorreram seis horas após a inoculação (h.a.i). A penetração ocorreu diretamente, por meio da formação do peg de penetração emitido da base do apressório, o qual penetrou na cutícula 48 h.a.i. O apressório não produziu o cone apressorial ao redor do poro de penetração. Foram observadas vesículas de infecção nas células epidérmicas 96 h.a.i. As mesmas estruturas fúngicas foram encontradas nas células epidérmicas e parenquimáticas do hospedeiro, após 120 h da inoculação. A estratégia de colonização de C. gloeosporioides foi do tipo hemibiotrófica intracelular. Termos para indexação: Colonização, penetração, pré-penetração, ultraestrutura. JEGER, 1992;JEFFRIES et al., 1990;PRUSKY;FREEMAN;DICKMAN, 2000). Although anthracnose symptoms may appear in the field, depending on the host and environmental conditions, Colletotrichum also cause quiescent infections in fruit, placing them amongst the most important postharvest pathogen. Thereby the pathogen infects the fruit still in the field, but symptoms appear only during ripening (JEFFRIES et al. 1990).Anthracnose is one of the main guava diseases in all guava-growing countries, causing considerable postharvest...

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Novel infection strategies of Colletotrichum acutatum on ripe blueberry fruit

Cited by 51 publications

References 29 publications

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Development and validation of an SPME-GC method for a degradation kinetics study of propiconazole I, propiconazole II and tebuconazole in blueberries in Concordia, the main production area of Argentina

Histopathology of Colletotrichum gloeosporioides on guava fruits (Psidium guajava L.)

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