Novel immobilization techniques of Acinetobacter (V2) and Paenibacillus (D9) bacterial strains for waste oil degradation

Abstract

Bacterial isolates used for the degradation of waste oils has gained greater interest in recent times since these isolates can be immobilized. However, it's their lack of reproducibility and continual usage that hinders its applicability. The aim of this work is to identify, purify, cultivate and immobilize bacterial isolates that are potential candidates to effectively and selectively degrade waste oils and waste oil blends and reuse them. In an attempt to render bacterium isolates applicable to degradation of lubricating and insulating oil in industrial processes this work investigates the immobilization (through support material, glutaldehyde activated amberlite, polyvinyl alcohol-sodium alginate and chitosan-sodium alginate matrix beads) of bacterial isolates. The data and observed trends show that developed immobilization techniques were successful in immobilizing bacterial isolate strains V2 and D9, with free cell degradation being most efficient (14.7-31.6%) followed by polyvinyl alcohol-sodium alginate (5.3-26.7%), chitosansodium alginate (8.8-0.3%) and amberlite-glutaldehyde (2.2-7.0%). The entrapment technique for immobilization of polyvinyl alcohol-sodium alginate and chitosan-sodium alginate proved to be more efficient than adsorption technique used for immobilization of amberlite-glutaldehyde. Free cell degradation was effective in degrading waste oil, but did so upon a single cycle with deterioration. Polyvinyl alcohol-sodium alginate bead matrix can be applied for degradative processes over a wide pH range and short incubation times while chitosan-sodium alginate is best suited for continuous long incubation periods. Reusability of both polyvinyl alcohol-sodium alginate and chitosan-sodium alginate beads are applicable up to a 10-cycle period.

Epiphytic and endophytic bacteria on Camellia oleifera phyllosphere: exploring region and cultivar effect

“…In addition, this study screened the microbes with the highest centrality value and played a key role in the microbial communities, such as Exiguobacterium , Methylobacterium , Paenibacillus , Pseudonocardia and Ochrobactrum for the phyllosphere community, and Acidovorax , Enterobacter , Amnibacterium , Methylobacterium and Burkholderia - Paraburkholderia for the leaf endophytic microbial community. Among them, Paenibacillus [ 49 ] is an important source of plant growth-promoting bacteria, which can directly promote plant growth through mechanisms such as nitrogen fixation, hormone production, siderophore secretion, and activation of mineral nutrients, and other mechanisms to defense against plant diseases. Burkholderia [ 50 ] can colonize the plant surface and rhizosphere, fix nitrogen, dissolve phosphorus, reduce plant ethylene levels, and produce auxin.…”

Epiphytic and Endophytic Bacteria on Camellia oleifera Phyllosphere: Exploring Region and Cultivar Effect.

“…In addition, this study screened the microbes with the highest centrality value and played a key role in the microbial communities, such as Exiguobacterium, Methylobacterium, Paenibacillus, Pseudonocardia and Ochrobactrum for the phyllosphere community, and Acidovorax, Enterobacter, Amnibacterium, Methylobacterium and Burkholderia-Paraburkholderia for the leaf endophytic microbial community. Among them, Paenibacillus [47] is an important source of plant growth-promoting bacteria, which can directly promote plant growth through mechanisms such as nitrogen fixation, hormone production, siderophore secretion, and activation of mineral nutrients, and other mechanisms to defense against plant diseases.…”

Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

“…Furthermore, its gel nature furnishes suitable porosity, allowing the continuous diffusion of substrates and byproducts [ 30 ]. In broader terms, the utilization of alginate gel immobilized with microbial biomass was recorded in different applications, such as alcoholic wine fermentation [ 31 ], dye decolorization [ 32 ], crack remediation [ 33 ] waste oil degradation [ 34 ] and heavy metal removal [ 24 , 35 ].…”