Novel <i>Camellia sinensis</i> <i>O-</i>Methyltransferase Regulated by CsMADSL1 Specifically Methylates EGCG in Cultivar “GZMe4”

Liu, Guangjin; Huang, Ke-Lin; Ke, Jia-Ping; Chen, Chen‐Hui; Bao, Guan‐Hu; Wan, Xiaochun

doi:10.1021/acs.jafc.2c06031

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…However, the introduction of a foreign gene and the regeneration of transgenic plants have posed technical challenges in Camellia . In the past two decades, transformed callus tissues have been obtained in tea plants [ 37 , 38 ], whereas transgenic regeneration plants have never been obtained. For the first time, we obtained callus tissues from C. azalea transformed with CaFT and CjFT , which represents a significant step forward in breaking the technical bottlenecks of establishing a genetic transformation system for ornamental Camellia .…”

Section: Discussionmentioning

confidence: 99%

Function of FT in Flowering Induction in Two Camellia Species

Wang,

Li,

Yin

et al. 2024

Plants

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FLOWERING LOCUS T (FT), belonging to the FT/TFL1 gene family, is an important gene regulating the flowering transition and inflorescence architecture during plant development. Given its importance to plant adaptation and crop improvement, FT has been extensively studied in related plant research; however, the specific role and underlying molecular mechanisms of FT in the continuous flowering of perennial plants remains elusive. Here, we isolated and characterized homologous FT genes from two Camellia species with different flowering-period phenotypes: CaFT was isolated from Camellia azalea, a precious species blooming in summer and flowering throughout the year, and CjFT was isolated from C. japonica, which blooms in winter and spring. The major difference in the genes between the two species was an additional five-amino acid repeat sequence in C. japonica. FT showed high expression levels in the leaves in both species from January to August, especially in April for C. japonica and in May for C. azalea. CaFT was expressed throughout the year in C. azalea, whereas CjFT was not expressed from September to December in C. japonica. The expression levels of FT in the floral buds were generally higher than those in the leaves. Overexpression of CaFT and CjFT in Arabidopsis indicated that both genes can activate downstream genes to promote flowering. Transgenic callus tissue was obtained by introducing the two genes into C. azalea through Agrobacterium-mediated transformation. Transcriptome and quantitative real-time polymerase chain reaction analyses indicated that both florigen FT genes promoted the expression of downstream genes such as AP1, FUL, and SEP3, and slightly up-regulated the expression of upstream genes such as CO and GI. The above results indicated that CaFT and CjFT played a role in promoting flowering in both camellia species. The expression pattern of CaFT in leaves suggested that, compared to CjFT, CaFT may be related to the annual flowering of C. azalea.

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Section: Discussionmentioning

confidence: 99%

Function of FT in Flowering Induction in Two Camellia Species

Wang,

Li,

Yin

et al. 2024

Plants

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Metabolomics of Tea Plants

Zhang,

Jia,

Ren

et al. 2024

Concepts and Strategies in Plant Sciences

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Metabolomic and genome-wide association studies drive genetic dissection and gene mining in tea plant

Jiang,

Zhao,

Gao

et al. 2024

HORTIC. ADV.

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As a vital reproductive organ, flowers significantly influence the yield, sensory quality, and breeding efficacy of Camellia sinensis. Many biological characteristics of tea plants are influenced by metabolites; however, our knowledge of metabolites in tea flowers is limited. To investigate the physiological basis and molecular mechanisms underlying tea flower metabolism, we integrated metabolomics and genome-wide association studies (GWAS) to analyze the metabolites present in the flowers of 171 tea genotypes. Untargeted metabolomic analysis detected 581 and 295 metabolites in positive and negative ionization modes, respectively. Twenty-seven distinct metabolites were observed between C. sinensis var. assamica (CSA) and C. sinensis var. sinensis (CSS). GWAS identified 1238 quantitative trait loci (QTL) associated with 505 metabolites. Some structurally related metabolites tended to share common QTL. Integrating GWAS findings with secondary mass spectrometry (MS/MS) fragmentation and haplotype analysis for metabolites (-)-epigallocatechin-3-(3"-O-methyl) gallate (EGCG-3''-O-ME), (-)-Epicatechin-3-(3''-O-methyl) gallate (ECG-3''-O-ME), Pos_1118, and Neg_365 (p-coumaroylquinic acid) resulted in the identification of three candidate genes (W07g015551, W08g018636, and W01g002625). Taken together, our findings provide a foundation for exploring comprehensive metabolic pathways in various tissues of C. sinensis.

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Novel Camellia sinensis O-Methyltransferase Regulated by CsMADSL1 Specifically Methylates EGCG in Cultivar “GZMe4”

Cited by 3 publications

References 34 publications

Function of FT in Flowering Induction in Two Camellia Species

Function of FT in Flowering Induction in Two Camellia Species

Metabolomics of Tea Plants

Metabolomic and genome-wide association studies drive genetic dissection and gene mining in tea plant

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