Novel hydroxyapatite nanorods improve anti-caries efficacy of enamel infiltrants

Neto, Davino M. Andrade; Carvalho, Elayne Valério; Rodrigues, Eliana Aparecida; Feitosa, Victor Pinheiro; Sauro, Salvatore; Mele, Giuseppe; Carbone, Luigi; Mazzetto, Selma Elaine; Rodrigues, Lidiany Karla Azevedo; Fechine, Pierre Basílio Almeida

doi:10.1016/j.dental.2016.03.026

Cited by 54 publications

(36 citation statements)

References 49 publications

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“…Fabrication of an enamel structure has been a high-priority target for more than 20 years, and a huge number of trials have been performed 11 . One reason for this is the promise of applying a protocol for artificial enamel fabrication to tooth repair 12, 13 .…”

Section: Introductionmentioning

confidence: 99%

Artificial enamel induced by phase transformation of amorphous nanoparticles

Onuma

Iijima

2017

Sci Rep

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Human tooth enamel has tightly packed c-axis-oriented hydroxyapatite (HAP: Ca10(PO4)6(OH)2) nanorods with high elastic modulus. Fabrication of an enamel architecture in vitro supports the repair of teeth using HAP; however, existing methods require complex and laborious steps to form an enamel-like structure. Here we present a very simple and effective technique for forming artificial enamel in near-physiological solution using a substrate composed of amorphous calcium phosphate (ACP) nanoparticles. Without any functionalized modification of the substrate surface, faint dissolution and successive phase transformation automatically induce formation of an intermediate layer of low-crystalline HAP nanoparticles, on which highly oriented HAP nanorods grow by geometrical selection. We also show that an enamel structure forms on a substrate of amorphous calcium carbonate when the surface nanoparticles react so as to form an intermediate layer similar to that in ACP. Our results demonstrate that there is a wide range of substrate choices for nanorod array formation. Contrary to current understanding, a stable surface designed in nanoscale is not essential for the growth of arranged guest crystals. Reactive amorphous nanoparticles and their transformation efficiently induce a nanorod array structure.

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Section: Introductionmentioning

confidence: 99%

Artificial enamel induced by phase transformation of amorphous nanoparticles

Onuma

Iijima

2017

Sci Rep

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“…The addition of fillers to a polymeric blend may lead to a different light-scattering pattern in the composite, resulting in differences in the number of converted monomers in the composite. 22,23 All CaP-containing groups presented lower DC values than the control group ( Table 2). The addition of filler resulted in increased opacity of the experimental sealants, and this could promote a reduction in light passage during polymerization.…”

Section: Discussionmentioning

confidence: 96%

“…3release, and later nucleation of mineral deposits. 22 Nano-sized HAp was used in the present study, whereas OCP and α-TCP were of micrometric size (Table 1). The different particle sizes used represent a limitation of the present study, since fillers were added by wt%; therefore, S HAp received a higher volume of particles.…”

Section: Discussionmentioning

confidence: 99%

Mineral deposition promoted by resin-based sealants with different calcium phosphate additions

et al. 2019

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The aim of this study was to evaluate the influence of different calcium phosphates (CaPs) on the physical, biological, and remineralizing properties of experimental resin-based sealants (RBSs). Triethylene-glycol dimethacrylate (90wt%) and bisphenol A-glycidyl methacrylate (10wt%) were used to produce resin-based sealants. Hydroxyapatite (S HAp), α-tricalcium phosphate (Sα-TCP) and octacalcium phosphate (S OCP) were added to the sealants in a 10wt% concentration. One group without CaPs was used as the control group (S CG). The degree of conversion (DC) was assessed with Fourier-transformed infrared spectroscopy, whereas cytotoxicity was tested with the HaCaT keratinocyte cell line. The ultimate tensile strength (UTS) was used to assess the mechanical strength of the experimental RBSs. Sealed enamel was used for colorimetric assay. Mineral deposition was assessed with Raman spectroscopy after 7, 14, and 28 days of sample immersion in artificial saliva. Scanning electron microscopy was used to analyze the surface morphology after 28 days of immersion. The addition of 10wt% of fillers significantly reduced the DC of sealants. S OCP groups showed reduced cell viability. Higher UTS was found for Sα-TCP and S HAp. The color analysis showed that S GC and demineralized teeth presented higher mismatches with the sound tissue. Mineral deposition was observed for S HAp and Sα-TCP after 7 days, with increased phosphate content and mineral deposits for S HAp after 28 days. RBS with the addition of 10% HAp promoted increased mineralization in vitro after 28 days, and did not affect cell viability, DC, mechanical properties, or RBS color in the enamel.

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“…It can remineralize the incipient carious lesions (20). Several studies have evaluated the remineralizing efficacy of nHA for demineralized enamel and dentin lesions (21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

Mineral Content Identification of White Spot Lesions Around Orthodontic Brackets Following the Use of Different Concentrations of Nano-Hydroxyapatite Mouthwash And Fluoride Gel

Talaei

Hoseini

Ghorbani

et al. 2019

Middle East J Rehabil Health Stud

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Background: Dental caries is a preventable infectious disease. White spot lesions (WSLs) are incipient caries with high prevalence during orthodontic treatment. They develop on the enamel surface around the orthodontic brackets due to food impaction and difficult oral hygiene maintenance. Fluoride is renowned for its cariostatic properties. Objectives: This study aimed to assess the mineral content of white spot lesions around orthodontic brackets following the use of different concentrations of nano-hydroxyapatite mouthwash and fluoride gel. Methods: Fifty sound-extracted premolars with artificially induced WSLs were randomly divided into 5 groups (n = 10) for the application of 1.23% fluoride gel, 1%, 5%, and 10% nHA mouthwash, and artificial saliva (control group). All groups, except for the control group, were subjected to the application of gel or mouthwashes twice with a one-week interval, each time for 24 hours. The mineral content of all teeth was assessed using scanning electron microscopy (SEM)/energy-dispersive X-ray spectrometer (EDX). Results: Maximum calcium uptake was noted in fluoride gel (P < 0.001) and 10% nHA (P < 0.001) groups. Maximum phosphorus uptake was noted in 10% nHA (P < 0.001) followed by fluoride gel (P < 0.001) and 5% nHA (P < 0.001) groups but the difference among the afore-mentioned three groups was not significant (P = 0.437). Maximum fluoride uptake was observed in the fluoride gel group with significant differences compared with other groups (P < 0.001). Conclusions: Fluoride gel and 10% nHA mouthwash can be effectively used for remineralization of WSLs.

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Novel hydroxyapatite nanorods improve anti-caries efficacy of enamel infiltrants

Cited by 54 publications

References 49 publications

Artificial enamel induced by phase transformation of amorphous nanoparticles

Artificial enamel induced by phase transformation of amorphous nanoparticles

Mineral deposition promoted by resin-based sealants with different calcium phosphate additions

Mineral Content Identification of White Spot Lesions Around Orthodontic Brackets Following the Use of Different Concentrations of Nano-Hydroxyapatite Mouthwash And Fluoride Gel

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