Novel hyaluronic acid–chitosan nanoparticles as non-viral gene delivery vectors targeting osteoarthritis

Lu, Huading; Zhao, Huiqing; Wang, Kun; Liu, Lulu

doi:10.1016/j.ijpharm.2011.08.046

Cited by 124 publications

(87 citation statements)

References 34 publications

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“…The HA/CSplasmid NPs showed a significantly higher transfection efficiency than CS-plasmid NPs without lowering the viability of transfected cells. 86 Similarly a CS-graft-PEI (CP)/DNA NPs have also been used as non-viral gene vectors in both chondrocytes and synoviocytes. The CS-graft-PEI copolymers efficiently carried the pDNA inside chondrocytes and synoviocytes without lowering the cell viability.…”

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confidence: 99%

Molecular targets in arthritis and recent trends in nanotherapy

Roy¹,

Kanwar²,

Kanwar³

2015

IJN

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Due to its severity and increasing epidemiology, arthritis needs no description. There are various forms of arthritis most of which are disabling, very painful, and common. In spite of breakthroughs in the field of drug discovery, there is no cure for arthritis that can eliminate the disease permanently and ease the pain. The present review focuses on some of the most successful drugs in arthritis therapy and their side effects. Potential new targets in arthritis therapy such as interleukin-1β, interleukin-17A, tumor necrosis factor alpha, osteopontin, and several others have been discussed here, which can lead to refinement of current therapeutic modalities. Mechanisms for different forms of arthritis have been discussed along with the molecules that act as potential biomarkers for arthritis. Due to the difficulty in monitoring the disease progression to detect the advanced manifestations of the diseases, drug-induced cytotoxicity, and problems with drug delivery; nanoparticle therapy has gained the attention of the researchers. The unique properties of nanoparticles make them highly attractive for the design of novel therapeutics or diagnostic agents for arthritis. The review also focuses on the recent trends in nanoformulation development used for arthritis therapy. This review is, therefore, important because it describes the relevance and need for more arthritis research, it brings forth a critical discussion of successful drugs in arthritis and analyses the key molecular targets. The review also identifies several knowledge gaps in the published research so far along with the proposal of new ideas and future directions in arthritis therapy.

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confidence: 99%

Molecular targets in arthritis and recent trends in nanotherapy

Roy¹,

Kanwar²,

Kanwar³

2015

IJN

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“…A few studies have reported the development of nanodelivery systems for gene therapy with plasmid DNA. For example, Lu et al 68 described the use of hybrid hyaluronic acid (HA)/ CS NPs as gene delivery vectors to transfer exogenous genes into primary chondrocytes for the treatment of joint diseases. In the study, HA/CS plasmid-DNA NPs were synthesized through the complex coacervation of the cationic polymers with a plasmid-expressing enhanced green fluorescent protein (EGFP).…”

Section: Nanodelivery Of Gene Therapies For Bone Diseasesmentioning

confidence: 99%

“…These results suggest that HA/CS NPs could be an effective nonviral vector for gene delivery to chondrocytes. 68 However, further testing in vivo is needed to fully assess the effectiveness of this delivery system on gene delivery.…”

Section: Nanodelivery Of Gene Therapies For Bone Diseasesmentioning

confidence: 99%

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

Wu²,

Chen³

et al. 2013

IJN

159

111

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Nanotechnology is a vigorous research area and one of its important applications is in biomedical sciences. Among biomedical applications, targeted drug delivery is one of the most extensively studied subjects. Nanostructured particles and scaffolds have been widely studied for increasing treatment efficacy and specificity of present treatment approaches. Similarly, this technique has been used for treating bone diseases including bone regeneration. In this review, we have summarized and highlighted the recent advancement of nanostructured particles and scaffolds for the treatment of cancer bone metastasis, osteosarcoma, bone infections and inflammatory diseases, osteoarthritis, as well as for bone regeneration. Nanoparticles used to deliver deoxyribonucleic acid and ribonucleic acid molecules to specific bone sites for gene therapies are also included. The investigation of the implications of nanoparticles in bone diseases have just begun, and has already shown some promising potential. Further studies have to be conducted, aimed specifically at assessing targeted delivery and bioactive scaffolds to further improve their efficacy before they can be used clinically.

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“…Vectors are normally required for the stabilization and efficient delivery of miRNAs. 19 Nonviral gene-delivery systems, such as chitosan (CS)/hyaluronic acid (HA) nanoparticles, [20][21][22][23] have been proposed as safer alternatives to viral vectors due to their good biocompatibility, biodegradability, high stability, and minimal host immune response. 20 The positive charges of CS contribute to electrostatic interactions with negatively charged substances, which are also responsible for the capacity of CS to interact with the negatively charged cell surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…22,27 A single application of CS presents a lack of stability due to its low water solubility at physiological pH, 28,29 and the inclusion of an anionic polymer (HA) appears to increase the stability of the particles in plasma and induce an even size distribution of nanoparticles. 23,[28][29][30] In addition, HA has the ability to bind various cellular receptors, such as CD44, which is expressed in normal mammalian cells and cancerous cells. 30 This ability would increase the transfection efficiency of target cells and reduce side effects.…”

Section: Introductionmentioning

confidence: 99%

Microarc-oxidized titanium surfaces functionalized with microRNA-21-loaded chitosan/hyaluronic acid nanoparticles promote the osteogenic differentiation of human bone marrow mesenchymal stem cells

Wang¹,

Wu²,

Feng³

et al. 2015

IJN

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Dental implants have been widely used for the replacement of missing teeth in the clinic, but further improvements are needed to meet the clinical demands for faster and tighter osseointegration. In this study, we fabricated safe and biocompatible chitosan (CS)/hyaluronic acid (HA) nanoparticles to deliver microRNA-21 (miR-21) and thereby accelerate osteogenesis in human bone marrow mesenchymal stem cells (hBMMSCs). The CS/HA/miR-21 nanoparticles were cross-linked with 0.2% gel solution onto microarc oxidation (MAO)-treated titanium (Ti) surfaces to fabricate the miR-21-functionalized MAO Ti surface, resulting in the development of a novel coating for reverse transfection. To characterize the CS/HA/miR-21 nanoparticles, their particle size, zeta potential, surface morphology, and gel retardation ability were sequentially investigated. Their biological effects, such as cell viability, cytotoxicity, and expression of osteogenic genes by hBMMSCs on the miR-21-functionalized MAO Ti surfaces, were evaluated. Finally, we explored appropriate CS/HA/miR-21 nanoparticles with a CS/HA ratio of 4:1 and N/P ratio 20:1 for transfection, which presented good spherical morphology, an average diameter of 160.4±10.75 nm, and a positive zeta potential. The miR-21-functionalized MAO Ti surfaces demonstrated cell viability, cytotoxicity, and cell spreading comparable to those exhibited by naked MAO Ti surfaces and led to significantly higher expression of osteogenic genes. This novel miR-21-functionalized Ti implant may be used in the clinic to allow more effective and robust osseointegration.

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Novel hyaluronic acid–chitosan nanoparticles as non-viral gene delivery vectors targeting osteoarthritis

Cited by 124 publications

References 34 publications

Molecular targets in arthritis and recent trends in nanotherapy

Molecular targets in arthritis and recent trends in nanotherapy

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

Microarc-oxidized titanium surfaces functionalized with microRNA-21-loaded chitosan/hyaluronic acid nanoparticles promote the osteogenic differentiation of human bone marrow mesenchymal stem cells

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Novel hyaluronic acid–chitosan nanoparticles as non-viral gene delivery vectors targeting osteoarthritis

Cited by 124 publications

References 34 publications

Molecular targets in arthritis and recent trends in&nbsp;nanotherapy

Molecular targets in arthritis and recent trends in&nbsp;nanotherapy

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

Microarc-oxidized titanium surfaces functionalized with microRNA-21-loaded chitosan/hyaluronic acid nanoparticles promote the osteogenic differentiation of human bone marrow mesenchymal stem cells

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Molecular targets in arthritis and recent trends in nanotherapy

Molecular targets in arthritis and recent trends in nanotherapy