Novel human polyomaviruses in pregnancy: Higher prevalence of BKPyV, but no WUPyV, KIPyV and HPyV9

Csoma, Eszter; Sápy, Tamás; Mészáros, Beáta; Gergely, Lajos

doi:10.1016/j.jcv.2012.07.009

Cited by 19 publications

(20 citation statements)

References 24 publications

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“…With the exception of JCPyV we did not find any known or unknown HPyVs in the samples using the RCA method. These results are consistent with data from others, who either could not detect HPyV-6, -7, -9, KIPyV, WUPyV, TSPyV or MWPyV in urine samples using real-time PCR [44,45] or reported detection only rarely and at very low concentrations (HPyV-9, KIPyV and WUPyV) [3,9,46,47]. …”

Section: Discussionsupporting

confidence: 92%

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Kluba

Linnenweber-Held²,

Heim³

et al. 2015

Intervirology

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Objectives: Multiple novel human polyomaviruses (HPyVs) have been discovered in the last few years. These or other, unknown, nephrotropic HPyVs may potentially be shed in urine. Methods: To search for known and unknown HPyVs we investigated BKPyV-negative urine samples from 105 renal transplant recipients (RTR) by rolling circle amplification (RCA) analysis and quantitative JCPyV PCR. Clinical data was analysed to identify risk factors for urinary polyomavirus shedding. Results: In 10% (11/105) of the urine samples RCA with subsequent sequencing revealed JCPyV, but no other HPyV sequences. Using quantitative JCPyV PCR, 24% (25/105) of the samples tested positive. Overall sensitivities of RCA of 44% (11/25) in detecting JCPyV in JCPyV DNA-positive urine and 67% (10/15) for samples with JCPyV loads >10,000 copies/ml can be assumed. Despite frequent detectable urinary shedding of JCPyV in our cohort, this could not be correlated with clinical risk factors. Conclusion: Routine urinary JCPyV monitoring in BKPyV-negative RTR without suspected polyomavirus-associated nephropathy might be of limited diagnostic value. As RCA works in a sequence-independent manner, detection of novel and known polyomaviruses shed in sufficient quantities is feasible. High-level shedding of HPyVs other than BKPyV or JCPyV in the urine of RTR is unlikely to occur.

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Section: Discussionsupporting

confidence: 92%

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Kluba

Linnenweber-Held²,

Heim³

et al. 2015

Intervirology

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“…Accordingly, the TSPyV early and late promoters were among the strongest of the HPyV in A64 and A549 cells. HPyV9 and HPyV10 are rarely detected in throat and nasal swabs (Csoma et al, 2012;Yu et al, 2012). HPyV9 promoter activity was relatively weak in A549, A64 and HSC-3 cells, while the HPyV10 late promoter had relatively high activity compared with the other HPyV promoters in A549 cells.…”

Section: Discussionmentioning

confidence: 95%

“…BKPyV, JCPyV and TSPyV can be found in the kidney (Doerries, 2006;Fischer et al, 2012), whereas KIPyV, MCPyV, HPyV9 and STLPyV DNA can be detected in urine (Csoma et al, 2012(Csoma et al, , 2015Husseiny et al, 2010;Lim et al, 2013;Mertz et al, 2010;Rockett et al, 2013). The BKPyV, MCPyV and TSPyV early and late promoters were among the strongest in HEK293 cells.…”

Section: Discussionmentioning

confidence: 99%

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Moens

Ghelue

Ludvigsen

et al. 2015

Journal of General Virology

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Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines Little is known about cell tropism of the novel HPyVs, and cell cultures allowing virus propagation are lacking. Because viral tropism partially depends on the interaction of cellular transcription factors with the viral promoter, we monitored the promoter activity of all known HPyVs. Therefore, we compared the relative early and late promoter activity of the BK polyomavirus (BKPyV) (WW strain) with the corresponding activities of the other HPyVs in 10 different cell lines derived from brain, colon, kidney, liver, lung, the oral cavity and skin. Our results show that the BKPyV, MCPyV, TSPyV and HPyV12 early promoters displayed the strongest activity in most cell lines tested, while the remaining HPyV had relative low early promoter activity. HPyV12 showed the highest late promoter activity of all HPyVs in most cell lines, but also the BKPyV, MCPyV and TSPyV late promoters belonged to the stronger ones among HPyVs. The HPyVs with weak early promoter activity had in general also weak late promoter activity, except for HPyV10 whose late promoter was relatively strong in six of the 10 cell lines. A 20 bp deletion in the promoter of an HPyV12 variant significantly affected both early and late promoter activity in most cell lines. In conclusion, our findings suggest which cell lines may be suitable for virus propagation and may give an indication of the cell tropism of the HPyVs.

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“…Little is known about the cell tropism of this virus, but viral sequences are predominantly found in respiratory tract secretions, with prevalence ranging between 0.5 and 6.5 % (Babakir-Mina et al, 2013). KIPyV DNA has occasionally been amplified from paranasal tissue (Babakir-Mina et al, 2009b), tonsil (Babakir-Mina et al, 2009b;Astegiano et al, 2010), lymphoid tissue (Sharp et al, 2009), lung tissue (Babakir-Mina et al, 2009c;Teramoto et al, 2011), stool (Allander et al, 2007;Babakir-Mina et al, 2009a;Bialasiewicz et al, 2009;Kantola et al, 2009;Mourez et al, 2009;Li et al, 2013), brain (Barzon et al, 2009b), eyebrow hair (Hampras et al, 2015), normal skin (Hampras et al, 2015) and blood and plasma (Barzon et al, 2009a;Babakir-Mina et al, 2010;Csoma et al, 2012;Touinssi et al, 2013). Immunohistochemical assay of spleen tissue from a 42-year-old human immunodeficiency virus (HIV)-positive male with a monoclonal antibody against the capsid protein VP1 of KIPyV stained positive, but the identity of the KIPyV VP1-positive cell type could not be determined (Siebrasse et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the non-coding control region of polyomavirus KI isolated from nasopharyngeal samples from patients with respiratory symptoms or infection and from blood from healthy blood donors in Norway

Song

Ghelue

Ludvigsen

et al. 2016

Journal of General Virology

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Seroepidemiological studies showed that the human polyomavirus KI (KIPyV) is common in the human population, with age-specific seroprevalence ranging from 40-90 %. Genome epidemiological analyses demonstrated that KIPyV DNA is predominantly found in respiratory tract samples of immunocompromised individuals and children suffering from respiratory diseases, but viral sequences have also been detected in brain, tonsil, lymphoid tissue studies, plasma, blood and faeces. Little is known about the sequence variation in the non-coding control region of KIPyV variants residing in different sites of the human body and whether specific strains dominate in certain parts of the world. In this study, we sequenced the non-coding control region (NCCR) of naturally occurring KIPyV variants in nasopharyngeal samples from patients with respiratory symptoms or infection and in blood from healthy donors in Norway. In total 86 sequences were obtained, 44 of which were identical to the original isolated Stockholm 60 variant. The remaining NCCRs contained one or several mutations, none of them previously reported. The same mutations were detected in NCCRs amplified from blood and nasopharyngeal samples. Some patients had different variants in their specimens. Transient transfection studies in HEK293 cells with a luciferase reporter plasmid demonstrated that some single mutations had a significant effect on the relative early and late promoter strength compared with the Stockholm 60 promoter. The effect of the NCCR mutations on viral replication and possible virulence properties remains to be established. INTRODUCTIONPolyomaviruses are non-enveloped viruses with a circular dsDNA genome of approximately 5000 bp. These viruses are common in birds and mammals, and recently the first complete fish polyomavirus genome has been published (Johne et al., 2011;Peretti et al., 2015). Their genome can †These authors contributed equally to this work.The GenBank/EMBL/DDBJ accession numbers for the sequences of the mutant NCCRs are KU564911-564952. ã 2016 The Authors Printed in Great Britain 1647Journal of General Virology (2016), 97, 1647-1657 DOI 10.1099 be divided into three functional regions: the early region, encoding regulatory proteins required for transcription and viral DNA replication; the late region, encoding the capsid proteins; and interspersed between these the non-coding control region (NCCR), encompassing the origin of replication and the promoters controlling transcription of the early and late genes, respectively (DeCaprio & Garcea, 2013). The first human polyomaviruses, BK and JC, were detected using histology or cytology followed by electron microscopy, and their existence was reported in 1971 (Gardner et al., 1971; Padgett et al., 1971). The development of new techniques such as high-throughput sequencing, rolling circle amplification and polymerase chain reaction with degenerate primers has led in recent years to the discovery of new polyomaviruses that can infect humans. KI polyomavirus, named after the researchers' instit...

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Novel human polyomaviruses in pregnancy: Higher prevalence of BKPyV, but no WUPyV, KIPyV and HPyV9

Cited by 19 publications

References 24 publications

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Characterization of the non-coding control region of polyomavirus KI isolated from nasopharyngeal samples from patients with respiratory symptoms or infection and from blood from healthy blood donors in Norway

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