Novel Human and Mouse Homologs of Saccharomyces cerevisiae DNA Polymerase η

Abstract

The Saccharomyces cerevisiae RAD30 gene encodes a novel eukaryotic DNA polymerase, pol eta that is able to replicate across cis-syn cyclobutane pyrimidine dimers both accurately and efficiently. Very recently, a human homolog of RAD30 was identified, mutations in which result in the sunlight-sensitive, cancer-prone, Xeroderma pigmentosum variant group phenotype. We report here the cloning and localization of a second human homolog of RAD30. Interestingly, RAD30B is localized on chromosome 18q21.1 in a region that is often implicated in the etiology of many human cancers. The mouse homolog (Rad30b) is located on chromosome 18E2. The human RAD30B and mouse Rad30b mRNA transcripts, like many repair proteins, are highly expressed in the testis. In situ hybridization analysis indicates that expression of mouse Rad30b occurs predominantly in postmeiotic round spermatids. Database searches revealed genomic and EST sequences from other eukaryotes such as Aspergillus nidulans, Schizosaccharomyces pombe, Brugia malayi, Caenorhabditis elegans, Trypanosoma cruzi, Arabidopsis thaliana, and Drosophila melanogaster that also encode putative homologs of RAD30, thereby suggesting that Rad30-dependent translesion DNA synthesis is conserved within the eukaryotic kingdom.

Mutagenic and Nonmutagenic Bypass of DNA Lesions byDrosophila DNA Polymerases dpolη and dpolι

“…: 81-75-753-7555; Fax: 81-75-753-7564; E-mail: todo@ house.rbc.kyoto-u.ac.jp. 1 The abbreviations used are: TLS, translesion DNA synthesis CPD, cis-syn-cyclobutane pyrimidine dimer; (6 -4)PP, pyrimidine-pyrimidone XPV/RAD30A gene (21,22)), pol (encoded by RAD30B (10,23)), and pol (encoded by DINB1 (24)). In addition, the human protein hRev1 is a homologue of yeast REV1 (25).…”

Genome scan stratified by the presence of anti-double-stranded DNA (dsDNA) autoantibody in pedigrees multiplex for systemic lupus erythematosus (SLE) establishes linkages at 19p13.2 (SLED1) and 18q21.1 (SLED2)

“…28 One of the mitogen-activated protein kinase enzymes (MAPK 4) (18q12-21), which their possible interaction pathways with DNA methyltransferase were mentioned above and the anti-apoptotic Bcl-2 gene are located here.…”

DNA repair, DNA replication and human disorders: A personal journey

“…Shortly afterwards, Roger Woodgate (who had done his PhD at Sussex with Bryn Bridges in 1980s), told me that he had found a gene related to Rad30 in yeast that was a good candidate for the XPV gene. We sequenced this gene in several XPV cell lines but did not find any mutations -the gene turned out to encode DNA polymerase [66], which is closely related to polymerase , the real XPV gene that was identified by the Hanaoka and Prakash groups in June 1999 [67,68].…”