Novel Heparin Receptor Transmembrane Protein 184a Regulates Angiogenesis in the Adult Zebrafish Caudal Fin

Farwell, Sara Lynn N.; Reylander, Kimberly G.; Iovine, M. Kathryn; Lowe-Krentz, Linda J.

doi:10.3389/fphys.2017.00671

Cited by 13 publications

(17 citation statements)

References 44 publications

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“…TMEM184A (also called Sdmg1) localizes to endosomes in mouse Sertoli cells and is required for the normal localization of the plasma membrane SNARE protein Stx2 ( Best et al, 2008 ). TMEM184A also acts as a heparin receptor in vascular cells and regulates angiogenesis ( Farwell et al, 2017 ; Pugh et al, 2016 ). TMEM184B localizes to recycling endosomes in mouse neurons and is important for the maintenance of synaptic architecture ( Bhattacharya et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein

Liu

Yamasaki

et al. 2018

eLife

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The ubiquitin-like protein Atg8, in its lipidated form, plays central roles in autophagy. Yet, remarkably, Atg8 also carries out lipidation-independent functions in non-autophagic processes. How Atg8 performs its moonlighting roles is unclear. Here we report that in the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae, the lipidation-independent roles of Atg8 in maintaining normal morphology and functions of the vacuole require its interaction with a vacuole membrane protein Hfl1 (homolog of human TMEM184 proteins). Crystal structures revealed that the Atg8-Hfl1 interaction is not mediated by the typical Atg8-family-interacting motif (AIM) that forms an intermolecular β-sheet with Atg8. Instead, the Atg8-binding regions in Hfl1 proteins adopt a helical conformation, thus representing a new type of AIMs (termed helical AIMs here). These results deepen our understanding of both the functional versatility of Atg8 and the mechanistic diversity of Atg8 binding.

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Section: Discussionmentioning

confidence: 99%

Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein

Liu

Yamasaki

et al. 2018

eLife

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“…Smc3 knockdown validation was confirmed by preparing MO1, MO2 and Std-MO injected fin lysates as described in Farwell et al (2017) . For evaluating the protein expression, western blotting technique using fluorescent secondary antibody was used as previously described ( Farwell and Lowe-Krentz, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Cohesin mediates Esco2-dependent transcriptional regulation in zebrafish regenerating fin model of Roberts syndrome

2017

Self Cite

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Robert syndrome (RBS) and Cornelia de Lange syndrome (CdLS) are human developmental disorders characterized by craniofacial deformities, limb malformation and mental retardation. These birth defects are collectively termed cohesinopathies as both arise from mutations in cohesion genes. CdLS arises due to autosomal dominant mutations or haploinsufficiencies in cohesin subunits (SMC1A, SMC3 and RAD21) or cohesin auxiliary factors (NIPBL and HDAC8) that result in transcriptional dysregulation of developmental programs. RBS arises due to autosomal recessive mutations in cohesin auxiliary factor ESCO2, the gene that encodes an N-acetyltransferase which targets the SMC3 subunit of the cohesin complex. The mechanism that underlies RBS, however, remains unknown. A popular model states that RBS arises due to mitotic failure and loss of progenitor stem cells through apoptosis. Previous findings in the zebrafish regenerating fin, however, suggest that Esco2-knockdown results in transcription dysregulation, independent of apoptosis, similar to that observed in CdLS patients. Previously, we used the clinically relevant CX43 to demonstrate a transcriptional role for Esco2. CX43 is a gap junction gene conserved among all vertebrates that is required for direct cell-cell communication between adjacent cells such that cx43 mutations result in oculodentodigital dysplasia. Here, we show that morpholino-mediated knockdown of smc3 reduces cx43 expression and perturbs zebrafish bone and tissue regeneration similar to those previously reported for esco2 knockdown. Also similar to Esco2-dependent phenotypes, Smc3-dependent bone and tissue regeneration defects are rescued by transgenic Cx43 overexpression, suggesting that Smc3 and Esco2 cooperatively act to regulate cx43 transcription. In support of this model, chromatin immunoprecipitation assays reveal that Smc3 binds to a discrete region of the cx43 promoter, suggesting that Esco2 exerts transcriptional regulation of cx43 through modification of Smc3 bound to the cx43 promoter. These findings have the potential to unify RBS and CdLS as transcription-based mechanisms.

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“…According to Cawley (2011), risk factors for obesity and the cardiovascular system are extremely widespread among urban adults in particular, which requires urgent policies to prevent the growth of chronic illness. Farwell et al (2017) demonstrated that the incidence of cardiovascular disease and its risk factors are two to three times higher in the urban area compared to rural study subjects. The study also shows that among industry employees, 27% of them were overweight and 41% face obesity.…”

Section: Determinants Of Health Policiesmentioning

confidence: 96%

Determinants of Health Policies in the Field of Cardiovascular Policies

Todosia¹

2020

Proceedings of the 13th Economics &Amp; Finance Virtual Conference, Prague

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The theme of public health is one with a very strong impact on the entire population. The problems in this sector affect every patient or potential patient, which is why public attention is very high on this topic. The interest in addressing this issue arises from the need for the balanced health status of the population, and health policies can support this goal. Public Health Policies refer to three aspects, based on the health system performance concept developed by the World Health Organization: population health status, citizens' satisfaction with the performance of the health system and the extent to which the system provides financial protection. Thus, the field of study of health policies is a general interest, and health policies in the field of cardiovascular medicine also taking into account that cardiovascular disease is the leading cause of death worldwide, according to statistics. This article aims to address the importance of health policies from the perspective of their determinants, given the influence of the population's illnesses on the development of health policies.

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Novel Heparin Receptor Transmembrane Protein 184a Regulates Angiogenesis in the Adult Zebrafish Caudal Fin

Cited by 13 publications

References 44 publications

Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein

Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein

Cohesin mediates Esco2-dependent transcriptional regulation in zebrafish regenerating fin model of Roberts syndrome

Determinants of Health Policies in the Field of Cardiovascular Policies

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