Novel glycosyltransferase genes involved in the acetan biosynthesis of Acetobacter xylinum

Ishida, Takehiko; Sugano, Yasushi; Shoda, Makoto

doi:10.1016/s0006-291x(02)00663-0

Cited by 19 publications

(9 citation statements)

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“…It is completely absent in K. europaeus LMG 18494, whereas the majority of these genes is missing in the K. hansenii strains, which was additionally verified using tblastn (Figure b). The most important seems to be the absence of aceA ( gumD) , which is thought to initiate acetan biosynthesis by transferring a glucosyl‐1‐phosphate residue from UDP‐glucose to an undecaprenyl‐phosphate lipid carrier anchored in the inner membrane (Brandt et al., ; Ishida, Sugano, & Shoda, ). This would implicate that the K. hansenii strains and K. europaeus LMG 18494 should not produce acetan.…”

Section: Resultsmentioning

confidence: 99%

“…The gene symbols relate to putative functions: polD (rmlD) — dTDP ‐4‐dehydrorhamnose reductase; polC (rmlC) — dTDP ‐4‐dehydrorhamnose 3,5‐epimerase; e.g.,—endoglucanase; gumE —polymerization or export protein; gumF —acyltransferase; gumK —glucuronosyltransferase; aceR —rhamnosyl transferase; aceQ —glucosyl transferase; aceP —glucosyl transferase; aceM ( ugd )— UDP ‐glucose 6‐dehydrogenase; aceF ( mpg )—mannose‐phosphate‐guanyl transferase; aceA ( gumD )— UDP ‐glucose:undecaprenyl‐phosphate glucose‐1‐phosphate transferase; aceB ( gumM )—glycosyltransferase; aceD ( gumC )—putative tyrosine‐protein kinase; aceC ( gumH )—glycosyltransferase; aceE —polysaccharide transporter or flippase; aceG —putative beta‐barrel porin 2 family protein; aceH ( gumB )—polymerization and export protein; aceI —acyltransferase; polA ( aceJ; rmlB )— dTDP ‐glucose 4,6‐dehydratase; polB ( rmlA )—glucose‐1‐phosphate thymidylyltransferase. Annotation is based on homology with gum ‐like gene cluster in Kozakia baliensis and acetan biosynthesis cluster from K. sucrofermentans DSM 15973 (Brandt et al., ; Ishida, Sugano, & Shoda, ). (c) Organization and conservation of the levan operon in K. kakiaceti JCM 25156, K. xylinus NBRC 13693, and Ga. diazotrophicus PA l 5.…”

Section: Resultsmentioning

confidence: 99%

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Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Ryngajłło

Kubiak

Jędrzejczak-Krzepkowska

et al. 2018

MicrobiologyOpen

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Komagataeibacter species are well‐recognized bionanocellulose ( BNC ) producers. This bacterial genus, formerly assigned to Gluconacetobacter , is known for its phenotypic diversity manifested by strain‐dependent carbon source preference, BNC production rate, pellicle structure, and strain stability. Here, we performed a comparative study of nineteen Komagataeibacter genomes, three of which were newly contributed in this work. We defined the core genome of the genus, clarified phylogenetic relationships among strains, and provided genetic evidence for the distinction between the two major clades, the K . xylinus and the K. hansenii . We found genomic traits, which likely contribute to the phenotypic diversity between the Komagataeibacter strains. These features include genome flexibility, carbohydrate uptake and regulation of its metabolism, exopolysaccharides synthesis, and the c‐di‐ GMP signaling network. In addition, this work provides a comprehensive functional annotation of carbohydrate metabolism pathways, such as those related to glucose, glycerol, acetan, levan, and cellulose. Findings of this multi‐genomic study expand understanding of the genetic variation within the Komagataeibacter genus and facilitate exploiting of its full potential for bionanocellulose production at the industrial scale.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Ryngajłło

Kubiak

Jędrzejczak-Krzepkowska

et al. 2018

MicrobiologyOpen

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“…The R and S strains are interconvertible by spontaneous mutation (Matsushita, Ebisuya, Ameyama, & Adachi, 1992). The genetic study of polysaccharides in acetic acid bacteria has shown that the acs (Saxena, Kudlicka, Okuda, & Brown, 1994) and the bcs (Wong et al, 1990) operons, in addition to ORF2 gene (Nakai, Nishiyama, Kuga, Sugano, & Shoda, 2002), are involved in cellulose biosynthesis, and the aceRQP operon in acetan biosynthesis in Ga. xylinus (Ishida, Sugano, & Shoda, 2002). Moreover, a gene cluster, polABCDE, required for pellicle formation in the R strain of A. tropicalis SKU1100, has been identified (Deeraksa et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of pellicle polysaccharides of Acetobacter tropicalis SKU1100 wild type and mutant strains

Ali¹,

Akakabe²,

Moonmangmee³

et al. 2011

Carbohydrate Polymers

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“…Bacterial CPS and EPS are a focus of study, as they are involved in human and plant pathogenesis, and human-and plant-bacterium interactions; they are also involved in biofilm formation, which may be a part of the stress response to, for example, antibiotics (Sutherland, 1982;Roberts, 1996). Polysaccharide biosynthetic pathways involving glycosyltransferases have also been studied (Ishida et al, 2002;Whitfield & Paiment, 2003).…”

Section: Introductionmentioning

confidence: 99%

Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100

et al. 2005

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Acetobacter tropicalis SKU1100 produces a pellicle polysaccharide, consisting of galactose, glucose and rhamnose, which attaches to the cell surface. This strain forms two types of colony on agar plates: a rough-surfaced colony (R strain) and a mucoid smooth-surfaced colony (S strain). The R strain forms a pellicle, allowing it to float on the medium surface in static culture, while the S strain does not. The pellicle is an assemblage of cells which are tightly associated with capsular polysaccharides (CPS) on the cell surface. In this study, a gene required for pellicle formation by the R strain was investigated by transposon mutagenesis using Tn10. The resulting mutant, designated Pel-, has a smooth-surfaced colony and a defect in pellicle formation, as for the S strain. The mutant produced polysaccharide which was instead secreted into the culture medium as extracellular polysaccharide (EPS). An ORF was identified at the Tn10 insertion site, designated polE, upstream of which polABCD genes were also found. The deduced amino acid sequences of polABCD showed a high level of homology to those of rfbBACD which are involved in dTDP-rhamnose synthesis, whereas polE had a relatively low level of homology to glycosyltransferase. In this study a polB (rfbA) disruptant was also prepared, which lacked both CPS and EPS production. A plasmid harbouring the polE or polB genes could restore pellicle formation in the Pel(-) mutant and S strains, and in the DeltapolB mutant, respectively. Thus both polE and polB are evidently involved in pellicle formation, most likely by anchoring polysaccharide to the cell surface and through the production of dTDP-rhamnose, respectively. The Pel- and DeltapolB mutants were unable to grow in static culture and became more sensitive to acetic acid due to the loss of pellicle formation. Additionally, this study identified the mutation sites of several S strains which were spontaneously isolated from the original culture and found them to be concentrated in a sequence of 7 C residues in the coding sequence of polE, with the deletion or addition of a single C nucleotide.

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Novel glycosyltransferase genes involved in the acetan biosynthesis of Acetobacter xylinum

Cited by 19 publications

References 30 publications

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Structural characterization of pellicle polysaccharides of Acetobacter tropicalis SKU1100 wild type and mutant strains

Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100

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