Novel genes in brain tissues of EAE-induced normal and obese mice: Upregulation of metal ion-binding protein genes in obese-EAE mice

Hasan, Mahbub; Seo, Jiyeon; Rahaman, Asiqur; Min, Hophil; Kim, Ki Hun; Park, Ju-Hyung; Sung, Changmin; Son, Junghyun; Kang, Min‐Jung; Jung, Byung Hwa; Park, Won Sang; Kwon, Oh‐Seung

doi:10.1016/j.neuroscience.2016.12.002

Cited by 22 publications

(14 citation statements)

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“…Among the increased proteins, we found proteins of the immunoglobulin family and a member of the serpin family of serine protease inhibitors, whose functions are not clearly understood ( Silverman et al, 2001 ). The decreased proteins are a neuroendocrine member of the secretogranin family, a player in the innate immune response, small leucine-rich proteoglycan, and members of the complement system ( Taupenot et al, 2003 ; Deckx et al, 2016 ; Hasan et al, 2017 ). However, as none of these proteins harbors a transmembrane domain, they are unlikely to be direct BACE2 substrates, and the observed changes are likely due to the indirect effects of BACE2 absence (in the background of Bace1 −/− ).…”

Section: Resultsmentioning

confidence: 99%

BACE2 distribution in major brain cell types and identification of novel substrates

et al. 2018

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Section: Resultsmentioning

confidence: 99%

BACE2 distribution in major brain cell types and identification of novel substrates

et al. 2018

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“…The disease was induced in male C57BL/6J mice by following protocols reported previously by our group . Briefly, the mice were injected with 150 µg of MOG(33–55) (MEVGWYRSPFSRVVHLYRNGK) emulsified in complete Freund's adjuvant containing 400 µg H37Ra Mycobacterium tuberculosis ; pertussis toxin (200 ng) was then injected intraperitoneally on day 0 and day 2 post immunization.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Proteome Analysis of Brain Subregions and Spinal Cord from Experimental Autoimmune Encephalomyelitis Mice by TMT‐Based Mass Spectrometry

et al. 2019

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Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS); its cause is unknown. To understand the pathogenesis of MS, researchers often use the experimental autoimmune encephalomyelitis (EAE) mouse model. Here, the aim is to build a proteome map of the biological changes that occur during MS at the major onset sites—the brain and the spinal cord. Quantitative proteome profiling is performed in five specific brain regions and the spinal cord of EAE and healthy mice with high‐resolution mass spectrometry based on tandem mass tags. On average, 7400 proteins per region are quantified, with the most differentially expressed proteins in the spinal cord (1691), hippocampus (104), frontal cortex (83), cerebellum (63), brainstem (50), and caudate nucleus (41). Moreover, region‐specific and commonly expressed proteins in each region are identified and bioinformatics analysis is performed. Pathway analysis reveals that protein clusters resemble their functions in disease pathogenesis (i.e., by inducing inflammatory responses, immune activation, and cell–cell adhesion). In conclusion, the study provides an understanding of the pathogenesis of MS in the EAE animal model. It is expected that the comprehensive proteome map of the brain and spinal cord can be used to identify biomarkers for the pathogenesis of MS.

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“…After 12 h of food deprivation, fasting body weight was precisely examined and then all the animals were anaesthetized with ether. Ether was used as the anesthetic due to its high efficiency, low cost and easy manipulation, thus it was still widely used in the animal experiments [7, 31, 32]. In consideration that the use of ether as an anesthetic might pose a safety hazard to researchers, a similar small equipment as previously described had been made and used to reduce the safety hazard [33].…”

Section: Methodsmentioning

confidence: 99%

Modulation of the gut microbiota by the mixture of fish oil and krill oil in high-fat diet-induced obesity mice

Cui

Gao

et al. 2017

PLoS ONE

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Previous studies confirmed that dietary supplements of fish oil and krill oil can alleviate obesity in mice, but the underlying mechanism remains unclear. This study aims to discern whether oil treatment change the structure of the gut microbiota during the obesity alleviation. The ICR mice received high-fat diet (HFD) continuously for 12 weeks after two weeks of acclimatization with a standard chow diet, and the mice fed with a standard chow diet were used as the control. In the groups that received HFD with oil supplementation, the weight gains were attenuated and the liver index, total cholesterol, triglyceride and low-density lipoprotein cholesterol were reduced stepwise compared with the HFD group, and the overall structure of the gut microbiota, which was modulated in the HFD group, was shifted toward the structure found in the control group. Moreover, eighty-two altered operational taxonomic units responsive to oil treatment were identified and nineteen of them differing in one or more parameters associated with obesity. In conclusion, this study confirmed the effect of oil treatment on obesity alleviation, as well as on the microbiota structure alterations. We proposed that further researches are needed to elucidate the causal relationship between obesity alleviation and gut microbiota modulation.

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Novel genes in brain tissues of EAE-induced normal and obese mice: Upregulation of metal ion-binding protein genes in obese-EAE mice

Cited by 22 publications

References 51 publications

BACE2 distribution in major brain cell types and identification of novel substrates

BACE2 distribution in major brain cell types and identification of novel substrates

Quantitative Proteome Analysis of Brain Subregions and Spinal Cord from Experimental Autoimmune Encephalomyelitis Mice by TMT‐Based Mass Spectrometry

Modulation of the gut microbiota by the mixture of fish oil and krill oil in high-fat diet-induced obesity mice

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