Novel fungal phenylpyruvate reductase belongs to d-isomer-specific 2-hydroxyacid dehydrogenase family

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse

“…NADH was only detected in cultures with sorghum bagasse hydrolysate, and the NAD + levels were not significantly different among the three cultivation conditions. NADPH availability limits PhLA production in E. coli cells, as NADPH is a cofactor for PhLA synthesis by PPR (Fujii et al, 2011). The supply of NADPH for PhLA synthesis is largely dependent on the activities of G6P and 6PG dehydrogenases, which catalyze rate-limiting steps in the PPP.…”

“…PhLA-producing E. coli strain GK1 (Kawaguchi et al, 2014), which expresses the phenylpyruvate reductase gene (pprA) derived from W. fluorescens TK1 (Fujii et al, 2011), was aerobically cultured at 37°C in Luria-Bertani medium (Sambrook and Russell, 2001) containing 20 lg mL À1 kanamycin.…”

Fermentation of aromatic lactate monomer and its polymerization to produce highly thermoresistant bioplastics

“…A complementary DNA fragment encoding W. fluorescens pprA (AB621792) was amplified using PCR and the pprA-f (5′-AAGGAGCCTTCGCCTCTATGAAAAAGCCTCAGGTCCTTA-3′) and pprA-r (5′-GTCGACTCAAACTACAAGATTCATTTCTT-3′) primers. 9 The gene promoter of C. glutamicum sp B (PcspB) was amplified using the csp-f (5′-GGATCCAAATTCCTGTGAATTAGCTGATT) and csp-r (5′-TAAGGACC TGAGGCTTTTTCATAGAGGCGAAGGCTCCTT-3′) primers. These DNA fragments were fused using PCR, digested with BamHI and SalI, and cloned into the C. glutamicum-Escherichia coli shuttle vector pPK4.…”

“…This study created artificial metabolic pathways for efficient fermentation of biomass glucose to 4-hydroxyphenyllactic acid using a combination of tyrosineoverproducing bacterium and phenylpyruvate reductase (PPR) from the phenyllactic acid-producing fungus Wickerhamia fluoresens, which can reduce 4-hydroxyphenylpyruvate, as well as phenylpyruvate. 9 The carboxylic group of the resulting DHPA was protected for subsequent use as a diol monomer for the polyesters. The polyesters produced by methylated DHPA polycondensation with a series of aliphatic and aromatic diacid chlorides exhibited a very high T g of 130°C.…”

Phenyllactic Acid: A Potential Antimicrobial Compound in Lactic acid Bacteria

“…These D-type of enzymes exhibit broad substrate specificity including PPA [17]. Fujii et al [37] discovered a novel fungal strain, Wickerhamia fluorescens TK1 which produced novel enzyme known as PPA reductase that reduced PPA to D-PhLA using either NADPH or NADH [37]. This enzyme has more preference for NADPH than NADH.…”