Novel Functions of the Matricellular Proteins Osteopontin and Osteonectin/SPARC

Sodek, Jaro; Zhu, Baoqian; Huynh, My Hang; Brown, Theodore L.; Ringuette, Maurice J.

doi:10.1080/713713529

Cited by 31 publications

(35 citation statements)

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“…FITC-labeled peptide 4.2 added to the media of differentiating EBs at day 7 became concentrated at the site of the peripheral endoderm by day 8, providing further support of a role for SPARC during PE differentiation. While we could not determine whether the peptide was internalized, intracellular activities for SPARC have been suggested in a number of studies (Huynh et al 2000(Huynh et al , 2004Sodek et al 2002;Yan et al 2005;Wilson et al 2006;Tang and Tai 2007).…”

Section: Discussionmentioning

confidence: 93%

Recombinant mouse SPARC promotes parietal endoderm differentiation and cardiomyogenesis in embryoid bodies

Hrabchak

Ringuette

Woodhouse

2008

Biochem. Cell Biol.

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In the absence of leukemia inhibitory factor, murine embryonic stem cells cultured in vitro spontaneously aggregate to from three-dimensional embryoid bodies that differentiate to produce hematopoietic, endothelial, muscle, and neuronal cell lineages in a manner recapitulating the events of early embryogenesis. Cardiomyogenesis in embryoid bodies was recently demonstrated to be promoted by PYS-2-derived native SPARC (secreted protein, acidic and rich in cysteine), whose expression is upregulated in parietal endoderm at the onset of the epithelial to mesenchymal transition. Here, we confirm the stimulatory effects of mouse SPARC on cardiomyogenesis using a recombinant baculovirus-produced protein (rmSPARC). Embryoid bodies cultured in the presence of glycosylated rmSPARC, or an unglycosylated peptide spanning the C-terminal EF-hand domain, developed greater numbers of beating cardiomyocytes than did time-matched controls, with enhanced expression of cardiac marker genes including Nkx2.5, Troponin, BMP-2, and MHCalpha. Histochemical analysis revealed an expansion of the peripheral endoderm, with thicker layers of extracellular matrix (ECM) material observed atop underlying cells. Embryoid bodies treated with SPARC also displayed increased adherence to polystyrene culture dishes, with enhanced expression of ECM mRNAs including collagen IValpha3, collagen IValpha5, and laminin alpha1. These results indicate that, in addition to the promotion of cardiomyogenesis, SPARC may also help regulate the molecular composition and organization of ECM secreted by the mesenchymal parietal endoderm.

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Section: Discussionmentioning

confidence: 93%

Recombinant mouse SPARC promotes parietal endoderm differentiation and cardiomyogenesis in embryoid bodies

Hrabchak

Ringuette

Woodhouse

2008

Biochem. Cell Biol.

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“…sOPN is secreted through the endoplasmic reticulum and Golgi, and exerts its effects by binding to the cell surface receptors. On the other hand, iOPN is co‐localized with the CD44‐ezrin‐radixin‐moesin (CD44‐ERM) complex that played a role in cell motility . iOPN is also involved in signal transduction pathways of innate immune receptors, such as Toll‐like receptors, and is translocated into the nucleus during mitosis …”

Section: Structure Receptors and Modifications Of Opnmentioning

confidence: 99%

Osteopontin: Versatile modulator of liver diseases

Nagoshi

2013

Hepatology Research

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Osteopontin (OPN) is a multifunctional protein, involved in pathological conditions including inflammation, immunity, angiogenesis, fibrosis and cancer progression in various tissues. Hepatic inflammation and fibrosis induced by feeding with a diet deficient in methionine and choline (MCD diet) were markedly attenuated in OPN knockout mice when compared with wild‐type mice in the model of non‐alcoholic steatohepatitis (NASH). Hepatic cholangiocytes, myofibroblastic stellate cells and natural killer T cells were suggested to secret OPN in mice fed an MCD diet. Plasma and hepatic OPN levels were significantly higher in patients with NASH with advanced fibrosis than in those with early fibrosis. Hepatic OPN mRNA level was correlated with hepatic neutrophil infiltration and fibrosis in patients with alcoholic liver diseases. In those with hepatocellular carcinoma (HCC), OPN levels in plasma and HCC were prognostic factors after liver resection or transplantation. Downregulation of OPN inhibited tumor growth and lung metastasis in nude mice implanted with HCC cells. The single nucleotide polymorphism in the promoter region of the OPN gene was shown to be associated with activity of hepatitis in chronic hepatitis C patients, prognosis in patients with HCC, and growth and lung metastasis of HCC xenografts in nude mice. OPN was reported to be a downstream effecter of Hedgehog pathway, which modulates hepatic fibrosis and carcinogenesis. This review focuses on the roles of OPN in hepatic inflammation, fibrosis and cancer progression. Further elucidation of cellular interactions and molecular mechanisms associated with OPN actions may contribute to development of novel strategies for treatment of the liver diseases.

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“…Other structural CNS‐associated ECM molecules include glycoproteins, such as tenascins (Hsia and Schwarzbauer 2005), thrombospondin (Bornstein et al. 2004), osteonectin/secreted protein acidic and rich in cysteine (Sodek et al. 2002) and various BM‐associated proteins.…”

Section: The Extracellular Matrix Of the Cnsmentioning

confidence: 99%

The extracellular matrix in multiple sclerosis pathology

Horssen

Dijkstra

Vries

2007

Journal of Neurochemistry

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The extracellular matrix (ECM) is a substrate upon which cells migrate, proliferate and differentiate. It is involved in the maintenance of cytoarchitecture, regulation of homeostasis, and it influences interactions between cells and molecules via specific receptors. Although a substantial body of knowledge has accumulated concerning the role of the ECM in peripheral tissues, little is known of the structure and function of the ECM in the CNS. However, marked changes in the expression of ECM constituents have been documented in various neurological disorders, including multiple sclerosis. This review focuses on the structure and function of the ECM in the CNS and in particular on the occurrence and involvement of ECM changes in the pathology of multiple sclerosis. Increased knowledge of the expression and functional role of ECM proteins in the CNS can lead to a better understanding of complex neurobiological processes both under normal as well as pathological conditions.

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Novel Functions of the Matricellular Proteins Osteopontin and Osteonectin/SPARC

Cited by 31 publications

References 0 publications

Recombinant mouse SPARC promotes parietal endoderm differentiation and cardiomyogenesis in embryoid bodies

Recombinant mouse SPARC promotes parietal endoderm differentiation and cardiomyogenesis in embryoid bodies

Osteopontin: Versatile modulator of liver diseases

The extracellular matrix in multiple sclerosis pathology

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