Novel foam separation column with hollow octagonal prismoids with sieve tray for purification of recombinant β-glucosidase

Abstract

In order to intensify the foam drainage and improve the enrichment ratio of recombinant β-glucosidase, a new foam separation equipment with a hollow octagonal prismoid with sieve tray (HOPST) was designed to separate recombinant β-glucosidase (GLEGB) from fermentation broth. The structural parameters (number, spacing, sieve diameter) of the foam separation internals and experimental parameters (temperature, initial protein concentration, gas flow rate, liquid volume) were optimized. Under the optimal conditions, the protein enrichment ratio was 2.46 ± 0.10, and the recovery rate of enzyme activity was 52.49 ± 2.50%. Based on the temperature sensitivity of elastin like polypeptide (ELP), the GLEGB foam solution was further purified with a purification ratio of 37.25 ± 0.60. The conformation results of purified GLEGB by CD, UV-vis and FT-IR showed that the structure of recombinant β-glucosidase was not changed during the purification process.