Novel fluorescent colloids as a DNA fluorescence probe

Abstract

Fluorescent perylene colloids in the 80-90 nm size range have been prepared by the reprecipitation method. These nanoparticles were modified by cetyltrimethylammonium bromide (CTAB) which inhibited their growth. The nanoparticles also readily interacted with DNA. The fluorescence emission was measured at lambda(ex)/ lambda(em)=400/565 nm. The fluorescence decrease of colloid-CTAB in aqueous solution was measured in the presence of nucleic acids. Under the optimum conditions, the ratio of fluorescence intensity in the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range 0.02-5.1 micro g mL(-1) for FS (fish sperm) DNA or CT (calf thymus) DNA. The detection limits were 0.01 micro g mL(-1) for FS DNA and 0.012 micro g mL(-1) for CT DNA, respectively. Based on this approach, a new quantitative method for DNA assay is presented in this paper.

Luminescent and hydrophilic LaF3–polymer nanocomposite for DNA detection

“…Numerous materials, such as organic fluorescent dyes (4)(5)(6)(7)(8), semiconductor quantum dots (9,10) and luminescent metal-ligand complexes (11)(12)(13)(14), have been widely used as fluorescent probes for DNA detection. However, there are still some intrinsic limitations in these materials.…”

Direct Condensation Method for the Preparation of Organic‐Nanoparticle Dispersions

“…The investigation of nanoparticles and microcrystals of such organic molecular materials is currently a rapidly growing field. Research on these particles is inspired by their high potential for applications in fields such as optoelectronic devices, [1][2][3] optical data storage, [4] chemo-and biosensors, [5,6] and photocatalysis. [7] Such nanoparticles and microcrystals of organic molecular materials very often represent an intermediate state, lying in between that of molecular solutions and bulk solid phases of the respective material.…”

Morphology control of organic luminescent microcrystals and approach of their optical properties

“…With a size considerably lower than that of bulk crystals, they exhibit a large proportion of surface molecules, and this characteristic feature is thought to govern many of their physical properties. Therefore, they find promising applications as luminescent probes for bioanalysis [1], as photocatalysts [2], and in the fields of photonics, non-linear optics and microelectronics [3].…”