Novel fluorescence-based method for rapid quantification of live bacteria in river water and treated wastewater

Abstract

Monitoring bacteria is essential for ensuring microbial safety of water sources, including river water and treated wastewater.

Advances in quantifying microbial contamination in potable water: Potential of fluorescence‐based sensor technology

“…Recently, fluorescence investigations have been further refined using targeted wavelength fluorescence field deployable , by targeting known excitation/emission pairs, such as tryptophan‐like fluorescence (Khamis et al, 2017; Sorensen, Vivanco, et al, 2018). While more novel experiments can be done using reagents to trigger a fluorescence response (e.g., Wang et al, 2022), field deployable fluorescence can capitalize on the intrinsic fluorescence of different molecules of organic matter (OM) to identify different functional groups present such as proteinaceous compounds and humic and fulvic acids. In situ fluorescence can also yield instantaneous data, at a fine temporal resolution.…”

Regrowth of Escherichia coli in environmental waters after chlorine disinfection: shifts in viability and culturability

“…For each dilution rate of samples, plates were prepared in triplicate. A LIVE/DEAD BacLight bacterial viability kit (L7012) was used to quantify viable E. coli, following our previous work 30 (details in Text S2 †). The viable and culturable E. coli counts in the control experiment showed a significant correlation (Pearson correlation coefficient r = 0.86, p < 0.05).…”

A highly sensitive SERS substrate based on a mesoporous Ag–TiO2 thin film for the detection of dye molecules

“…3,4 The spectroscopy-based detection tools such as fluorescence spectroscopy, IR spectroscopy, and Raman spectroscopy offer reliable specification of molecules in terms of their electronic, vibrational, and rotational energy levels. 5,6 However, the Raman signal is weak and fails to provide molecular information at the ultra-trace level. 7 Surface-enhanced Raman spectroscopy (SERS), unravelled by Fleishman et al , has received enormous attention due to its applications in multidisciplinary areas.…”