Novel fibrous collagen-based cream accelerates fibroblast growth for wound healing applications: in vitro and in vivo evaluation

Udhayakumar, Sivalingam; Shankar, K. Gopal; Sampath, S.; Rose, C.

doi:10.1039/c7bm00331e

Cited by 37 publications

(25 citation statements)

References 68 publications

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“…In the maturation phase, the continuity of the skin begins and the process of re-epithelisation continues [ 84 ]. In this process, Col supplies adequate nutrition directly to the wound, enhancing the repair mechanism and aiding in scar reduction [ 86 ].…”

Section: Roles Of Collagen and Gelatin In Wound Healing Phasesmentioning

confidence: 99%

Natural-Based Biomaterial for Skin Wound Healing (Gelatin vs. Collagen): Expert Review

et al. 2021

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Collagen (Col) and gelatin are most extensively used in various fields, particularly in pharmaceuticals and therapeutics. Numerous researchers have proven that they are highly biocompatible to human tissues, exhibit low antigenicity and are easy to degrade. Despite their different sources both Col and gelatin have almost the same effects when it comes to wound healing mechanisms. Considering this, the bioactivity and biological effects of both Col and gelatin have been, and are being, constantly investigated through in vitro and in vivo assays to obtain maximum outcomes in the future. With regard to their proven nutritional values as sources of protein, Col and gelatin products exert various possible biological activities on cells in the extracellular matrix (ECM). In addition, a vast number of novel Col and gelatin applications have been discovered. This review compared Col and gelatin in terms of their structures, sources of derivatives, physicochemical properties, results of in vitro and in vivo studies, their roles in wound healing and the current challenges in wound healing. Thus, this review provides the current insights and the latest discoveries on both Col and gelatin in their wound healing mechanisms.

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Section: Roles Of Collagen and Gelatin In Wound Healing Phasesmentioning

confidence: 99%

Natural-Based Biomaterial for Skin Wound Healing (Gelatin vs. Collagen): Expert Review

et al. 2021

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“…With the improvements in recombinant protein techniques, the research on high-quality and contaminant-free RHC has been growing by leaps and bounds. The RHC may produce a suitable biological dressing for burn wounds as an alternative to native collagen (Shilo et al, 2013;Udhayakumar et al, 2017;Cheng and Hu, 2019). Rutschmann et al (2014) coexpressed human type III collagen with virus-derived proline 4-hydroxylase in the E. coli expression system to obtain RHC with hydroxylated structure (Rutschmann et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Hybrid Freeze-Dried Dressings Composed of Epidermal Growth Factor and Recombinant Human-Like Collagen Enhance Cutaneous Wound Healing in Rats

Cheng

Huang

et al. 2020

Front. Bioeng. Biotechnol.

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Epidermal growth factor (EGF) is important for promoting skin repair and remodeling. Native collagen is also widely used as a scaffold for skin tissue engineering. The limitations of EGF include easy decomposition or inactivation, whereas native collagen is immunogenic and has poor solubility. Therefore, we constructed a freeze-dried dressing based on the recombinant human-like collagen (RHC) to act as a carrier for EGF (RHC/EGF freeze-dried dressing) and promote skin wound closure. Here, the freezedried dressing that combined EGF and RHC significantly enhanced the proliferation, adhesion, and spreading of NIH/3T3 fibroblasts and migration of HaCaT keratinocytes at the wound site. The physicochemical characteristics of the RHC/EGF freeze-dried dressing investigated using scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy, and differential scanning calorimetry revealed that it was a loose and porous cake that redissolved quickly. The molecular mechanisms involved in cell proliferation and angiogenesis were also assessed. The expression levels of the markers Ki-67, proliferating cell nuclear antigen, vascular endothelial growth factor, and cluster of differentiation 31 were significantly increased after treatment with the RHC/EGF freeze-dried dressing (P < 0.01, vs. RHC or EGF alone). This increase indicated that the RHC/EGF freeze-dried dressing significantly accelerated wound closure, re-epithelialization, and the orderly arrangement and deposition of collagen in the Sprague-Dawley rats with full-thickness skin defects. This work describes a significant step toward the development of wound environments conducive to healing, and the RHC/EGF freeze-dried dressing is a potential therapeutic strategy in wound management.

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“…Chitosan (80% deacetylated), was purchased from Sigma Aldrich (St. Louis, MO, USA). 3 g of wet collagen paste and 1 g of chitosan were blended and prepared as a cream according to the method of Udhayakumar et al [17].…”

Section: Preparation Of Collagen Formsmentioning

confidence: 99%

Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study

Babu

Srinivasan

Dhandapani³

et al. 2021

JPRI

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Aims: Natural biomaterials are more suitable than synthetic biomaterials for in vivo applications for treating damaged tissues. Collagen and chitosan are abundantly available natural biomaterials for wound dressings for tissue/wound repair. In this context, collagen-chitosan composite powder has been used to treat chronic wounds in Hansen disease (HD) patients. Place and Duration of Study: CSIR Central Leather Research Institute, Sardar Patel Road, Adyar, Chennai 600021, Southern Railway Headquarters Hospital, Constable Road, Ayanavaram, Chennai 600023, and Gremaltes Hospital, India between June 2013 and July 2020. Methodology: Collagen extracted from bovine rumen, a waste product of meat industry, and a commercially available chitosan were prepared as a composite powder (COL/CS) and applied to chronic wounds in HD patients after debridement and the wound contours were measured by planimetry. Biochemical parameters in blood samples were periodically assessed. Histopathology of wound tissue with Hematoxylin and Eosin and Masson’s Trichrome staining was studied. Matrix Metalloproteinase-9 (MMP9) levels before and after treatment were estimated. Results: Wound healing of 64.2% was obtained with COL/CS treatment and formation of granulation cells was observed early. Hemogram studies have been reported in a regression model with 95% confidence intervals. Histopathology revealed dense collagen fibres and continuity of sub-epithelial layer on 8th day. MMP-9 levels showed collagen integrity after treatment. Conclusion: The novel biocompatible, biodegradable COL/CS wound dressing is a promising biomaterial for management of chronic wounds in Hansen disease patients.

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Novel fibrous collagen-based cream accelerates fibroblast growth for wound healing applications: in vitro and in vivo evaluation

Cited by 37 publications

References 68 publications

Natural-Based Biomaterial for Skin Wound Healing (Gelatin vs. Collagen): Expert Review

Natural-Based Biomaterial for Skin Wound Healing (Gelatin vs. Collagen): Expert Review

Hybrid Freeze-Dried Dressings Composed of Epidermal Growth Factor and Recombinant Human-Like Collagen Enhance Cutaneous Wound Healing in Rats

Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study

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