Novel ferrocene‐containing triacyl derivative of resveratrol improves viability parameters in ovary cells

Abstract

Besides the use of resveratrol as a drug candidate, there are obstacles mainly due to its poor pharmacokinetic properties. Numerous studies are being conducted on the synthesis of resveratrol derivatives that exhibit enhanced biological activity. The aim of our research was to investigate activity of the newly synthesized ferrocene‐containing triacyl derivative of resveratrol to achieve cell protection from endo/exogenous ROS and reduction in cell death by assessing multiple endpoints. Our research showed that both resveratrol and the resveratrol derivatives (1–100 μM) lower the levels of ROS in CHO‐K1 cells. Resveratrol at doses <20 μM had little or no effect on cell proliferation, while at higher doses, a significant inhibitory effect on cell proliferation and viability has been noticed. The activity of the new derivative was significantly altered compared to resveratrol—cellular viability was not suppressed regardless of the concentration applied, and the extent of apoptosis was low. In summary, the new ferrocene‐resveratrol derivative has the potential to protect cells from oxidative stress due to its low cytotoxicity and retained antioxidant properties, whereas caution should be exercised with resveratrol at higher doses, as it significantly impairs cell viability and induces cell death. By linking ROS to specific diseases (relevance in neurodegenerative, cardiovascular, and neoplastic diseases), we can assume that the new resveratrol derivative can prevent or alleviate the mentioned disorders. Furthermore, recognition of the resveratrol derivative as an anti‐apoptotic chemical could be useful in the cultivation of various cell lines on a large scale in the industrial biotechnology.

Ouratea sp. and Virola surinamensis seed fats as new sources of lipids: Composition, physicochemical characterization, bioactive potential, and cytotoxicity

“…[55] CHO-K1 cells are also widely applied in toxicology research due to their non-tumor characteristics and stable karyotype. [56,57] The sensitivity of CHO-K1 cell line for detecting compounds that act on DNA was previously related by Aardema et al [58] Figure 4 shows the results obtained from the MTT assay using CHO-K1 cells in different concentrations (100, 500, and 1000 μg mL −1 ) of the fat samples at 24 h. Tests were also performed at 48 and 72 h of incubation with statistically similar results (unpublished results).…”

“…[ 55 ] CHO‐K1 cells are also widely applied in toxicology research due to their non‐tumor characteristics and stable karyotype. [ 56,57 ] The sensitivity of CHO‐K1 cell line for detecting compounds that act on DNA was previously related by Aardema et al. [ 58 ]…”

Novel Ferrocene-Containing Triacyl Derivative of Resveratrol Protects Ovarian Cells from Toxicity Caused by Ortho-Substituted Polychlorinated Biphenyls

“…To overcome these drawbacks, an infinite number of different polyphenol analogs and prodrugs have been synthesized, and various technological approaches have been developed that involve polyphenol incorporation into different delivery systems (e.g., liposomes, nanoparticles). Recently, our group demonstrated a beneficial effect of a novel resveratrol derivative, RF, on multiple cellular endpoints in ovary cell culture [8]. Guided by these positive results, the aim of this study was to explore the possible application of a new RF as a protective agent against the cytotoxic effects of PCBs.…”

“…Various structural modifications of RSV resulted in improved liposolubility in the cellular environment and thus in modified cellular response. Recently, a novel ferrocene-containing triacyl derivative of RSV (RF; Figure 1B) was synthesized by our group, and we have pointed out its beneficial effect on multiple cell viability parameters in ovary cell culture [8]. Compared with RSV, the novel derivative was non-toxic to non-cancerous ovarian cells with noticeable antioxidant potential, whereas RSV exerted cytotoxicity in doses > 20 µM.…”