Novel enzyme-membrane reactor for polysaccharide synthesis

Hicke, Hans‐Georg; Ulbricht, Mathias; Becker, Margot; Radosta, S.; Heyer, Arnd G.

doi:10.1016/s0376-7388(99)00116-7

Cited by 28 publications

(21 citation statements)

References 11 publications

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“…The resulting even intraporous functionalization of the track-etched membranes had also previously been obtained for amino-functional graft copolymer layers which then had been used for covalent enzyme immobilization; the obtained enzyme binding capacities and activities could be explained by an even coverage of the pore surface. [26,27] There is a clear correlation between the gravimetrically determined degree of functionalization and the carboxylic group amounts on the membranes, determined by reversible ionic dye binding (see Figure 7; cf. Table 2 and 3).…”

Section: Degree Of Functionalizationmentioning

confidence: 95%

“…[8,9,16,26,27] Effects of functionalization were detected by gravimetry (DG) and using the reversible dye binding to carboxylic groups.…”

Section: Identification Of Graft-copolymerization Conditionsmentioning

confidence: 99%

“…[14,15,17,19,25] Also, the resulting porous composite membranes may directly find interesting applications, for examples as enzyme-membrane reactor. [26,27] Furthermore, the knowledge about suited functionalization methods could be applied to the functionalization of other objects made from the same base polymer, for example components for ''labs-on-a-chip''.…”

Section: Introductionmentioning

confidence: 99%

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Photoreactive Functionalization of Poly(ethylene terephthalate) Track‐Etched Pore Surfaces with “Smart” Polymer Systems

Geismann

Ulbricht

2005

Macro Chemistry & Physics

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116

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Summary: Poly(ethylene terephthalate) (PET) track‐etched membranes with a pore diameter of ca. 700 nm, optionally surface‐functionalized to create a “carboxyl” or “amino” surface, were used for heterogeneous graft copolymerizations. Grafted poly(acrylic acid) acted as a pH responsive, “smart” polymer. To evaluate the surface‐specific initiation of graft copolymerizations unmodified and primary functionalized membranes were systematically combined with three differently charged benzophenone derivatives as photoinitiators, which were adsorbed on the PET surface prior to the reaction. The functionalized membranes thus obtained were characterized for chemical structure and permeability as a function of pH. The pore structure with a narrow size distribution and the resulting high sensitivity of the membrane permeability to the grafting functionalizations made the track‐etched PET membranes a very useful tool for analyzing structure and function of responsive grafted polymer layers. The primary functionalization of the base polymer with a molecular layer of a multifunctional alkylamine (“amino” surface) enhanced the efficiency of the subsequent graft copolymerization via photoinitiated hydrogen abstraction considerably because a high density of well accessible reactive groups had been introduced. The preadsorption of the photoinitiator on the base polymer surface was significantly improved by ionic interactions between the respective functional groups of the surface and the photoinitiator. Such a photoinitiator preadsorption, especially when combined with a reactive layer from the primary functionalization, enabled a very efficient and surface selective functionalization because a better control of grafting density and a reduction of photoinitiated side reactions along with a more efficient use of the photoinitiator were possible.Water permeabilities (pH 2 and pH 7) of unmodified and functionalized PET membranes for identification of optimal synthesis and evaluation conditions.magnified imageWater permeabilities (pH 2 and pH 7) of unmodified and functionalized PET membranes for identification of optimal synthesis and evaluation conditions.

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Section: Degree Of Functionalizationmentioning

confidence: 95%

“…[8,9,16,26,27] Effects of functionalization were detected by gravimetry (DG) and using the reversible dye binding to carboxylic groups.…”

Section: Identification Of Graft-copolymerization Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photoreactive Functionalization of Poly(ethylene terephthalate) Track‐Etched Pore Surfaces with “Smart” Polymer Systems

Geismann

Ulbricht

2005

Macro Chemistry & Physics

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116

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“…Porous membranes for enzymatic reactions for polysaccharide syntheses [95] were produced from commercial capillary pore membranes with diameters of 0.4, 1.0, and 3.0 lm. The enzyme (fructosyltransferase) was covalently bound to the surface by a previously photo-grafted layer of poly(2-aminoethyl methacrylate) to the membrane surface.…”

Section: Reviewsmentioning

confidence: 99%

Developments in Membrane Research: from Material via Process Design to Industrial Application

et al. 2006

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The aim of this review paper is to give an overview of the research activities of GKSS in the field of polymer based membranes. After summarizing the historic development of membrane science at GKSS, it describes different classes of polymeric materials for membranes, and the characterization of membranes. The design of membrane‐based separation processes followed by examples of applications will also be presented. In the last chapter we will try to give an outlook for the future activities in membrane research.

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“…An EMR based on surface-functionalized capillary pore (nuclear track-etched) membranes with enzymes immobilized on the pore walls had been demonstrated to be suited for the synthesis of oligo and polysaccharides [23,24]. In particular, a very interesting product, inulin with extraordinarily high MW and low polydispersity [25], could be obtained in vitro after covalent immobilization of fructosyltransferase (FTF) in the cylindrical membrane pores [23]. However, the main problem was that there is most probably the formation of an enzyme/inulin complex causing first a change in enzyme specificity and ultimately an extensive pore blocking.…”

Section: Introductionmentioning

confidence: 99%

Covalently coupled nanoparticles in capillary pores as enzyme carrier and as turbulence promoter to facilitate enzymatic polymerizations in flow-through enzyme–membrane reactors

Hicke¹,

Becker²,

Paulke³

et al. 2006

Journal of Membrane Science

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Novel enzyme-membrane reactor for polysaccharide synthesis

Cited by 28 publications

References 11 publications

Photoreactive Functionalization of Poly(ethylene terephthalate) Track‐Etched Pore Surfaces with “Smart” Polymer Systems

Photoreactive Functionalization of Poly(ethylene terephthalate) Track‐Etched Pore Surfaces with “Smart” Polymer Systems

Developments in Membrane Research: from Material via Process Design to Industrial Application

Covalently coupled nanoparticles in capillary pores as enzyme carrier and as turbulence promoter to facilitate enzymatic polymerizations in flow-through enzyme–membrane reactors

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