Novel electrochemical sensing platform based on a nanocomposite of PVA/PVP/RGO applied to IgG anti- Toxoplasma gondii antibodies quantitation

Abstract

This article describes the development of a new electrochemical platform composed by a polymer mixture and graphene oxide (GO). The working electrode of a screen-printed carbon electrode (SPCE) was modified with nanocomposite constituted by poly-vinyl alcohol (PVA), poly-vinylpyrrolidone (PVP) and GO, which was electrochemically reduced to obtain PVA/PVP/RGO/SPCE. The interactions and morphology of the PVA/PVP/GO nanocomposite were investigated by SEM, FTIR and UV-Vis. SEM images indicated an excellent dispersion of the GO sheets in the polymer matrix. Besides, FTIR and visible UV studies revealed strong interactions between polymer mixture and GO sheets. According to electrochemical studies, the new platform increased the electroactive surface area by a factor of 20.46 compared to the unmodified SPCE. Also, the PVA/PVP/RGO/SPCE had a fast electron kinetics transfer process with a value of k s = 9.6 s-1. The modified electrode was applied to the determination of IgG anti-T. gondii antibodies for the serological diagnosis of toxoplasmosis. The IgG anti-T. gondii antibodies quantification showed a detection limit of 0.012 U mL-1 , and the coefficients of variation intra-day and inter-day assays were lower than 4.5 % and 6.2 %, respectively. The electrochemical platform proved to be a sensitive and easily applicable tool applied to the serological diagnosis of toxoplasmosis. Therefore, the developed nanocomposite represents an excellent alternative for the electrochemical biosensor fabrication.

Polymers with Cyanoethyl Ether and Propanesulfonate Ether Side Chains for Solid-State Li-Ion Battery Applications

“…The presence of peaks at 2249 cm –1 (CN stretching), 1095 cm –1 (C–O–C stretching), and the greatly reduced intensity of the peak at 3400 cm –1 (O–H stretching) clearly reveal that partial transformation of −OH to −OCH 2 CH 2 CN occurred during the cyanoethylation of PVA and formed PVA–CN accordingly. Furthermore, in comparison with the spectrum of PVA–CN, the characteristic peak at 1258 cm –1 (C–OH stretching) is not observed in the PVA–CN–SO 3 spectrum . In addition, the PVA–CN–SO 3 spectrum also shows new bands at 1173, 1046, and 926 cm –1 , corresponding to S=O asymmetric, S=O symmetric, and C–S stretching, respectively. , Thus, these results provide supporting evidence for the successful formation of PVA–CN–SO 3 .…”

“…Furthermore, in comparison with the spectrum of PVA−CN, the characteristic peak at 1258 cm −1 (C−OH stretching) is not observed in the PVA−CN−SO 3 spectrum. 29 In addition, the PVA−CN−SO 3 spectrum also shows new bands at 1173, 1046, and 926 cm −1 , corresponding to S=O asymmetric, S=O symmetric, and C−S stretching, respectively. 30,31 Thus, these results provide supporting evidence for the successful formation of PVA−CN−SO 3 .…”

Novel Electrochemical Biosensing for Detection of Neglected Tropical Parasites of Animal Origin: Recent Advances

“…The presented probe showed a good potential towards the POC for T. gondii. Takara et al develop an electrochemical biosensing platform based on a nanocomposite of poly-vinyl alcohol/ poly-vinylpyrolidone and graphene oxide (PVA/PVP/RGO) for T. gondii diagnosis [71]. The LOD was 0.012 U/ml was reported with very less variation during intra-day and inter-day assays.…”

Electrochemical immunosensor based on gold-thionine for detection of subarachnoid hemorrhage biomarker

“…Although these measurement methods are able to achieve IL-6 detection to some extent, a variety of factors restrict further development, including complicated procedures requiring professional executors, low accuracy, time-consuming, and expensive instruments in the laboratory. The electrochemical immunoassay of specific antigen-antibody interactions has significant application in biomedical diagnostics ( Hou et al, 2019 ; Takara et al, 2019 ). Electrochemical immunosensors, which are designed to be based on biosensing technology, have many significant advantages over conventional detection methods, such as simple operation, convenient carrying, and high sensitivity ( Sun et al, 2020 ).…”