Novel Electroanalytical Technique for Determination of Trospium Hydrochloride

Abstract

The present work describes for the first time the fabrication of a novel disposable screen printed sensor for the potentiometric determination of trospium hydrochloride (TRO). The cited sensors were based on alpha cyclodextrin as molecular recognition element and multi walled carbon nanotubes (MWCNTs) as transducer. Comprehensive and deep investigations were done on the sensing membrane components including the nature of sensing material, cavity size, additives, and plasticizers in addition to nanomaterial. The fabricated sensors were highly sensitive, selective and accurate towards TRO with Nernstian slope 60.8± 0.5 mV decade -1 in the concentration range from 10 -6 to 10 -2 mol L -1 . This can be attributed to the incorporation of MWCNTs to the electrode matrix which accelerates response time and improves potential reading stability. The fabricated sensors were used for the potentiometric determination of TRO under batch and flow injection technique with agreeable recoveries compared with the standard methods. Moreover, the high selectivity of the sensors towards TRO in presence of its degradation products suggests application of this method as stability indicating technique for TRO quality control.

A Screen-Printed Potentiometric Sensor for Stability Indicating Assay and Real-Time Monitoring of Trospium Chloride Dissolution Profile in its Pharmaceutical Dosage Form

“…This will bring a leading change in forecasting the in-vivo dissolution performance of oral dosage forms with an ultimate goal of biowaiving the in vivo bioavailability and/or bioequivalence studies. For the best of our knowledge, only one electroanalytical method has been described in the literature for the determination of TRO in pharmaceutical and biological samples 48 but it didn't shed light on monitoring the dissolution of TRO in different media.…”

Novel approaches of erythrosine B as a food dye‐derived spectroscopic probe for assessing trospium chloride in raw material and dosage form

“…[2] Different documented methods have been published in TPM assessment including high performance liquid chromatography (HPLC) methods, [3][4][5][6] spectrophotometric methods, [7][8][9] and electrochemical methods. [10][11][12] The British Pharmacopoeia suggested a chromatographic method for estimating of TPM using C8 column and a mobile phase containing triethylamine: phosphoric acid:water:acetonitrile (0.1:0.3:70:30, v/v). [1] It is worth noting that the published methods have certain disadvantages.…”

“…[5,6] The low sensitivity of the reported spectrophotometric methods was a drawback in the methods, in addition to using nongreen caustic diluting solvents such as NaOH or HCl. [7,9] Electrochemical processes need costly chemicals such as silver nitrate, [10,11] in addition to the required experience to conduct the study. The suggested work has focused on developing simple and sensitive methods for estimating TPM in raw material and dosage forms.…”