Novel electroactive ferrocene-based covalent organic frameworks towards electrochemical label-free aptasensors for the detection of Cardiac Troponin I

An electrochemical aptasensor for methylamphetamine rapid detection by single-on mode based on competition with complementary DNA

“…Subsequently, 50 μL of the activated MB-Apt-S solution was added dropwise to the surface of the gold electrode and incubated at room temperature for 1 h. Ultimately, the MB-Apt-S was immobilized on the surface of the gold electrode through Au–S and we obtain the modified electrode. After that, the electrode was incubated in ethanol solution with 1 mmol/L MCH at room temperature for 2 h. The thiol molecules in the MCH solution could occupy the active sites on the GE surface to passivate the GE, which was conducive to the formation of a well-aligned DNA monolayer 29 . After a thorough wash, the modified electrode (MB-Apt-S/GE) was finally obtained.…”

Recent Progress of Covalent Organic Frameworks Applied in Electrochemical Sensors

“…The FcCOFNs based sensor showed a wide linear range from 10 fg mL −1 to 10 ng mL −1 with ultralow detection limit of 2.6 fg mL −1 of cTnI detection. 145 The prostate specific antigen (PSA) is the most optimal serum biomarker for screening and diagnosing prostate tumors. Li and co-workers developed an electrochemical sensor for PSA determination by employing black phosphorene (BPene) as an electrochemical platform and magnetic COFs as an electrochemical signal amplification.…”

“…The prepared electrode showed sensitive voltammetric response, while the electrochemical signal decreased significantly after cTnI was adsorbed on the surface of Fc-COFNs through H-bonding and π–π stacking interactions. The FcCOFNs based sensor showed a wide linear range from 10 fg mL –1 to 10 ng mL –1 with ultralow detection limit of 2.6 fg mL –1 of cTnI detection …”

Conductive polymer nanocomposites: recent advances in the construction of electrochemical biosensors

“…in biological samples including blood, serum and body fluids play an essential role in the diagnosis of diseases and guidance of clinical treatments. With the rapid development of clinical medicine, more and more biological targets are being verified to directly correspond to specific diseases or physiological state, such as glucose to diabetes, 1-4 troponin to myocardial infarction, [5][6][7][8] alpha fetoprotein to liver cancer, [9][10][11] prostate specific antigen to prostate cancer, 12 human mucin-1 to breast cancer, 13 amyloid-beta to Alzheimer's disease, 14 SARS-CoV-2 spike protein to COVID 19 disease. [15][16][17] Therefore, the accurate and fast detection of such small molecules or macromolecules justifies the keen interest shown by interdisciplinary researchers in the fields of biochemistry, chemistry, immunology, clinical medicine, engineering and materials science.…”