Novel Electrical Method for Early Detection of Viable Bacteria in Blood Cultures

Puttaswamy, Sachidevi; Lee, Byung Doo; Sengupta, Shramik

doi:10.1128/jcm.00369-11

Cited by 29 publications

(28 citation statements)

References 14 publications

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“…Whilst the leading cause of mortality is prematurity and its complications, the second is neonatal sepsis 2 . Continuous monitoring blood culture systems (CMBCS), such as BACTEC, are presently the "gold standard" for the detection of bacteraemia and sepsis in the clinical setting 3 . CMBCS monitors carbon dioxide levels in the suspension and a significant rise in these levels indicates the presence of viable bacteria in the suspension and therefore in the blood 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Continuous monitoring blood culture systems (CMBCS), such as BACTEC, are presently the "gold standard" for the detection of bacteraemia and sepsis in the clinical setting 3 . CMBCS monitors carbon dioxide levels in the suspension and a significant rise in these levels indicates the presence of viable bacteria in the suspension and therefore in the blood 3 . Antibiotics have been used extensively in the management of sepsis and are sometimes used empirically without identifying the causative organisms or knowing the antibiotic sensitivity, leading to development of resistance.…”

Section: Introductionmentioning

confidence: 99%

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Haemato-bacteriological profile and antibiogram of suspected cases of early onset sepsis in very low birth weight neonates

Rawat

Shukla

2019

Sri Lanka J Child Health

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Introduction: The current Indian neonatal mortality rate is 28 per 1000 live births. Whilst the leading cause of mortality is prematurity and its complications, the second is neonatal sepsis. Objectives:To describe spectrum of isolates in neonatal sepsis together with their antibiotic sensitivity pattern and significance of routine blood investigations for diagnosis of early onset septicaemia. Method:A cross-sectional study was performed in 100 neonates with risk factors for septicaemia after obtaining informed consent. Routine blood investigations such as complete blood count and Creactive protein (CRP) were done on the first day of life. Specificity, sensitivity, positive predictive value and negative predictive value of total leucocyte count, absolute neutrophil count and CRP were calculated. Blood culture was done using Bactec Peds Plus/F culture as a gold standard to diagnose septicaemia. The study was approved by the Institutional Ethics Committee. Results:Out of 100 neonates with suspected sepsis, 40 were found to have culture proven sepsis. CRP had the highest sensitivity (45%) and negative predictive value (71%), leucopenia had the highest positive predictive value (72%) and neutropenia had the highest specificity (91.7%). The common bacteria for early onset sepsis (EOS) were Klebsiella, Pseudomonas and methicillin resistant Staphylococcus Aureus (MRSA) contributing 17% each to the bacteriological profile. All the gram negative bacteria in our study were sensitive to gentamicin and gram positive bacteria were sensitive to nitrofurantoin, tigecycline, tetracycline, vancomycin, teicoplanin, and linezolid. _________________________________________ 1 Third Year M.D. Resident, 2 Additional Professor, The authors declare that there are no conflicts of interest Personal funding was used for the project. Open Access Article published under the Creative Commons Attribution CC-BY LicenseConclusions: This study showed that leucopenia, neutropenia and CRP are good indicators of sepsis, when used in combination. A negative CRP can exclude sepsis as it has the highest negative predictive value. This study also showed that gram negative bacilli (Klebsiella and Pseudomonas) were the common organisms associated with early onset neonatal sepsis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Haemato-bacteriological profile and antibiogram of suspected cases of early onset sepsis in very low birth weight neonates

Rawat

Shukla

2019

Sri Lanka J Child Health

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“…Here, we demonstrate the first integrated completely hands-free diagnostic multiplex assay for F. tularensis, B. anthracis, and Y. pestis. The assay detected each bacterial species at an LOD of Ͻ10 CFU/ml of blood, with a linear dynamic range of up to 10 4 CFU/ml, which is likely above the upper limit for bloodstream infections (28,29). The multiplexed TAP assay LOD was not compromised by the presence of multiple sets of primers and probes for all three targets, in contrast to our earlier studies on a single target (6,9).…”

Section: Discussionmentioning

confidence: 64%

Multiplex Detection of Three Select Agents Directly from Blood by Use of the GeneXpert System

et al. 2019

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Francisella tularensis, Bacillus anthracis, and Yersinia pestis are tier 1 select agents with the potential to rapidly cause severe disease. Rapid detection of these bacteria from patient samples at the point of care could contribute to improved clinical outcomes in the event of a bioterrorism attack. A multiplex nested PCR assay for detection of F. tularensis, B. anthracis, and Y. pestis directly from patient blood samples was developed using the GeneXpert system. The multiplex GeneXpert cartridge-based assay includes all necessary sample processing and amplification reagents. Blood samples spiked with different numbers of CFU were used to measure the analytical limit of detection (LOD) and dynamic range. Sensitivity was determined by testing spiked blood samples and negative-control blood in a blind manner. Specificity was determined by testing against nontarget pathogens and blood samples from clinical patients. The assay LOD was 8.5 CFU/ml for F. tularensis, 10 CFU/ml for B. anthracis, and 4.5 CFU/ml for Y. pestis. The sensitivity was 100% at the LOD for all three select agent bacteria in spiked patient blood samples. The assay specificity was 100% when it was tested against both nontarget pathogens and clinical patient blood samples. The total assay time was approximately 100 min. This automated assay, which is suitable for use at the point of care, identifies three select agents directly in blood without the need for enrichment with a high sensitivity within 100 min. This assay may enable rapid detection and treatment of patients infected with the target organisms in the event of a bioterrorism attack.

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“…In this study, we used a single dose (10 mg/kg) of AMPR-11 to evaluate its efficacy in a murine model of sepsis induced by 2 ϫ 10 7 to 8 ϫ 10 8 CFU of bacteria, which is commonly used for sepsis models. However, human sepsis is much more sensitive than that of mice in terms of inflammatory responses: 100 CFU/ml in blood induce sepsis in humans (50). The blood volume of the mice we used in this study (28 to 30 g) was approximately 1.5 to 2 ml, indicating that we injected bacteria at a rate of 2 ϫ 10 5 to 8 ϫ 10 7 times higher than the pathological concentration of bacteria in humans.…”

Section: Resultsmentioning

confidence: 91%

Romo1-Derived Antimicrobial Peptide Is a New Antimicrobial Agent against Multidrug-Resistant Bacteria in a Murine Model of Sepsis

Lee

You

Kim

et al. 2020

mBio

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To overcome increasing bacterial resistance to conventional antibiotics, many antimicrobial peptides (AMPs) derived from host defense proteins have been developed. However, there are considerable obstacles to their application to systemic infections because of their low bioavailability. In the present study, we developed an AMP derived from Romo1 (AMPR-11) that exhibits a broad spectrum of antimicrobial activity. AMPR-11 showed remarkable efficacy against sepsis-causing bacteria, including multidrug-resistant strains, with low toxicity in a murine model of sepsis after intravenous administration. It seems that AMPR-11 disrupts bacterial membranes by interacting with cardiolipin and lipid A. From the results of this study, we suggest that AMPR-11 is a new class of agent for overcoming low efficacy in the intravenous application of AMPs and is a promising candidate to overcome multidrug resistance. IMPORTANCE Abuse of antibiotics often leads to increase of multidrug-resistant (MDR) bacteria, which threatens the life of human beings. To overcome threat of antibiotic resistance, scientists are developing a novel class of antibiotics, antimicrobial peptides, that can eradicate MDR bacteria. Unfortunately, these antibiotics have mainly been developed to cure bacterial skin infections rather than others, such as life-threatening sepsis. Major pharmaceutical companies have tried to develop antiseptic drugs; however, they have not been successful. Here, we report that AMPR-11, the antimicrobial peptide (AMP) derived from mitochondrial nonselective channel Romo1, has antimicrobial activity against Gram-positive and Gram-negative bacteria comprising many clinically isolated MDR strains. Moreover, AMPR-11 increased the survival rate in a murine model of sepsis caused by MDR bacteria. We propose that AMPR-11 could be a novel antiseptic drug candidate with a broad antimicrobial spectrum to overcome MDR bacterial infection.

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Novel Electrical Method for Early Detection of Viable Bacteria in Blood Cultures

Cited by 29 publications

References 14 publications

Haemato-bacteriological profile and antibiogram of suspected cases of early onset sepsis in very low birth weight neonates

Haemato-bacteriological profile and antibiogram of suspected cases of early onset sepsis in very low birth weight neonates

Multiplex Detection of Three Select Agents Directly from Blood by Use of the GeneXpert System

Romo1-Derived Antimicrobial Peptide Is a New Antimicrobial Agent against Multidrug-Resistant Bacteria in a Murine Model of Sepsis

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