Novel DiR and SPIO nanoparticles embedded PEG-PLGA nanobubbles as a multimodalimaging contrast agent

Abstract

Abstract. Fluorescence dye DiR and superparamagnetic iron oxide nanoparticles (SPIONs) embedded in PEG-PLGA nanobubbles (DiR-SPIO-NBs) were produced using double emulsion method on a membrane of Shirasu porous glass (SPG). The nanobubbles encapsulated with DiR and SPIONs had a liquid core (perfluoropentane) and a PEG-PLGA shell. DiR-SPIO-NBs showed biocompatibility based on MTT cytotoxicity and hemolysis studies. The PFP encapsulated in the nanobubbles experienced phase transition under ultrasonic irradation. Nanobubbles dispersed well in saline over 3 months and the relaxivity was 127.9 mM -1 s -1 , suggesting that it could be used as a contrast agent in MRI. The MR and fluorescence images in vivo demonstrated that the signal intensity in the spleen and liver was significantly enhanced with the treatment of nanobubbles. In addition, results of ultrasound images suggested that the nanobubbles had persistent contrast ability. In conclusion, nanobubbles could be utilized as an US/MRI/fluorescence contrast agent.

Beyond Oncological Hyperthermia: Physically Drivable Magnetic Nanobubbles as Novel Multipurpose Theranostic Carriers in the Central Nervous System

“…The ADV favor the liquid to gas phase shift of perfluoropentane, increasing the US backscattering. The US imaging contrast ability of SPIONs decorated polymer nanobubbles was previously demonstrated by Luo et al [ 34 ].…”

<p>Anti-ST2 Nanoparticle Alleviates Lung Inflammation by Targeting ILC2s-CD4⁺T Response</p>

“…These properties have offered new prospects for novel magnetic nanoparticles that can improve treatment and diagnosis in a unique multifunctional system. 10 In this paper, we demonstrate that DiR-SPION conjugated to anti-ST2 blocking antibodies (anti-ST2 NPs) efficiently suppressed the development of asthma by block the function of ILC2s.…”

Stimuli-responsive nanobubbles for biomedical applications

“…198 The for ultrasound/MRI/fluorescence imaging. 158 Li et al in turn reported a multimodal PLGA-shell NB for combined NIR/MRI/ultrasound imaging of tumors by electrostatic absorption of oleylamine-/IR-780loaded hollow structures and the GdDTPA-BSA@5-FU complex to the NB shell together with folate as a targeting ligand. 142 Wang et al…”

“…142,158,159 Both Luo et al and Li et al reported the development of ultrasound-, magnetic-, and fluorescence-responsive NBs for multimodal imaging. The NBs were coated with PLGA to which a fluorescence dye and the magneticresponsive materials, such as SPIONs, were directly conjugated; or alternatively these compounds can be encapsulated in the NBs.…”