Novel developments in capillary electrophoresis miniaturization, sampling, detection and portability: An overview of the last decade

Kubáň, Pavel; Kubáň, Petr

doi:10.1016/j.trac.2023.116941

Cited by 24 publications

(5 citation statements)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The implementation of a CE platform, either in microchannels or fused silica glass as a POC device, began in the 1990s [109][110][111][112][113][114]; since then, several mini handheld prototypes have been manufactured [113][114][115][116][117]. One palmtop bioanalyzer has a laser-induced fluorescent detector-the authors affirm that this tiny device combines seamless design with low-cost construction, retailing for USD 500 [113].…”

Section: Potential Use Of Point-of-care Platforms For the Screening O...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

View full text Add to dashboard Cite

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage of formation, these disorders can be effectively treated, leading to successful outcomes; curative therapies can halt illnesses from advancing, thus improving the quality of life and long-term survival of the patient. Still, cutting-edge upgrades in precision technologies are necessary for early, reliable, affordable, and rapid disease detection, but also vital for the well-being of people and the future of global public health. The emerging role and utility of non-invasive and repeatable diagnostic test approaches for the detection of health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator or signature (e.g., proteins) to provide valuable information on an individual’s wellness and/or disease. Among the bodily secretions used for non-invasive diagnostics is sputum, a complex viscous gel-like biopolymeric network that has gained growing recognition as a rich source of biomarkers of airway infections and pulmonary diseases, and serves as a determinant to reveal other illnesses. As per the World Health Organization, the burden of respiratory conditions is exacerbated by factors ranging from considerable subjection to air pollution and occupational contaminants to tobacco smoking and second-hand smoke, in addition to poor socio-economic status. Due to the likely increase in these determinants, respiratory tract ailments are on the rise, affecting the health of many individuals, in addition to putting stress on healthcare facilities and services worldwide. The aim of this study was to perform a narrative review of sputum constituents with an emphasis on proteins and glycoproteins assessed as possible biomarkers of lung and other organ diseases. A search was conducted using mucus, sputum proteomics, sputum biomarkers, and point-of-care testing as keywords employing Google, PubMed (MEDLINE), and Web of Science, selecting the most referenced and related papers of the last decade. We, therefore, highlight the need to use expectorated or induced sputum specimens as a routine sample source for testing valuable protein biomarkers to diagnose these chronic disorders, predict inflammation and disease progression, as well as monitor the effectiveness of treatments. Further, we discuss the urgent need for fast and reliable point-of-care methods to detect and quantify crucial protein biomarkers in sputum specimens, and the limitations faced when dealing with their complex matrices.

show abstract

Section: Potential Use Of Point-of-care Platforms For the Screening O...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

View full text Add to dashboard Cite

show abstract

“…Among the other detection modes, the universal capacitively coupled contactless conductivity detection (C 4 D) is relatively frequently used in CE and MCE methods [211]. However, much more for small ions, such as acids, AAs [212,213], and other small biomolecules [214,215] than for peptides.…”

Section: Other Detection Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

View full text Add to dashboard Cite

This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

show abstract

“…that are adaptable to LC but may have been used specifically for a different separation technique (capillary electrophoresis [CE], gas chromatography [GC], ion mobility spectrometry [IMS], etc.). For specific details on microcontrollers and single-board computers as related to other liquid-phase separation techniques, indepth reviews on CE [14][15][16] and microfluidics [17][18][19] have also been published.…”

Section: Introductionmentioning

confidence: 99%

The utilization of open‐source microcontroller boards and single‐board computers in liquid chromatography

Piccolo,

Foster,

Parker

et al. 2024

J of Separation Science

View full text Add to dashboard Cite

Electronic system control of analytical instrumentation remains a critical aspect of modern measurement science. Within the field of liquid chromatography (LC), this is especially relevant for automation, module operation, detection, data acquisition, and data analysis. Increasingly, home‐built analytical tools used for liquid‐phase separations rely upon open‐source microcontrollers and single‐board computers to aid in simplifying these operations. In this review, we detail literature reported within the past 5 years in which these types of devices were used to advance various aspects of the LC research field, including sample preparation, instrument control, and data collection.

show abstract

Novel developments in capillary electrophoresis miniaturization, sampling, detection and portability: An overview of the last decade

Cited by 24 publications

References 130 publications

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

The utilization of open‐source microcontroller boards and single‐board computers in liquid chromatography

Contact Info

Product

Resources

About