Novel data set for retrospective biodosimetry using both conventional and FISH chromosome analysis after high accidental overexposure

Sevan'kaev, A.V.; Ik, Khvostunov; Mikhailova, G. F.; Golub, E. V.; Potetnya, O.I.; Shepel, N.N.; Nugis, V.Yu.; Nadejina, N. M.

doi:10.1016/s0969-8043(00)00062-2

Cited by 14 publications

(5 citation statements)

References 3 publications

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“…In contrast, when only doses <1 Gy are considered dose estimates made using translocations (6 years after accident) were exactly overlapped with the estimates made immediately following accident using dicentric analysis. A similar conclusion is made in the follow up of Chernobyl cohorts [22]. Concerning the persistence of translocations in experiments performed during 1992 until 1997, no significant difference could be detected [17,3], that indicates persistence of translocations.…”

Section: Comparison To Non-chernobyl Studiessupporting

confidence: 73%

“…The notion was to estimate the frequency and spectrum of chromosome aberrations (including specific cytogenetic markers of radiation exposure) and to help clinician for therapy regimen of the victims. In studies performed in the period of 1986-1987 in different groups dicentric analysis was used to estimate individual doses that ranged from 20 cGy to 980 cGy [22]. In the follow up studies in the same cohort or other groups (i.e.…”

Section: Post Chernobyl Experiences (In Human Studies)mentioning

confidence: 99%

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Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Darroudi

2012

Sixth International Conference on Environmental Mutagens in Human Populations

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Abstract. Human risk assessments at the low doses, and low dose rates and high doses following acute exposure to ionizing radiation are of prime importance in radiation protection. These issues are of continuing importance in respect of social/economic policy relating to the industrial and medical uses of ionizing radiation, and for risk assessment among people occupationally are being exposed to low and/or high LET radiation, such as astronauts, pilots, stewardess and nuclear power plant workers, as well as victims of radiation accidents. Consequently, several biological assays were developed and attempts were made to investigate formation of radiation induced chromosome aberrations and induction of genomic instability in human lymphocytes and fibroblasts. Fluorescence in situ hybridization (FISH) technique using chromosome, chromosome-arm, chromosome region, centromere and telomere specific DNA libraries has improved the resolution of detecting all classes of radiation induced chromosomal inter-and intra-changes. Consequently, has increased significantly the accuracy and detection limit of biological dosimetry. Newly obtained data indicate that (a) Premature chromosome condensation assay is a unique method to be used for immediate dose assessment at low (5cGy) as well as high doses (≥3 Gy) and can accurately discriminate between whole-and partial-body exposure in case of mass casualties and accidental over-exposure to high doses of ionizing radiation (b) FISH-based translocation assay has the potential to assess acute as well as chronic exposure in cases of accidental as well as occupational exposure to ionizing radiation, either immediately following exposure, or retrospectively by defining accumulative effects to red bone marrows. (c) there are distinct finger-prints (such as insertions and complex translocations) for high LET radiation in comparison to low LET radiation. The importance of these findings and future perspectives for biological dosimetry are discussed.

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Section: Comparison To Non-chernobyl Studiessupporting

confidence: 73%

Section: Post Chernobyl Experiences (In Human Studies)mentioning

confidence: 99%

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Darroudi

2012

Sixth International Conference on Environmental Mutagens in Human Populations

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“…Natarajan, A.T., Santos, S.J., Darroudi, F., Hadjidikova, V., Vermeulen, S., Chatterjee, S., Berg, M., Grigorova, M., Sakamoto-Hojo, E.T., Granath, F., et al For dicentrics the yield equation Y=0.0003+ 0.059D+ 0.043GD 2 was used, see text. a -this work, b -Nugis (24) , c -Sevan'kaev et al (25) , d -Salassidis et al (26) . 1 Doses estimated with G=1.…”

Section: Discussionmentioning

confidence: 81%

A cytogenetic follow-up of some highly irradiated victims of the Chernobyl accident

Sevan'kaev¹,

Lloyd²,

Edwards³

et al. 2004

Radiation Protection Dosimetry

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A follow-up of ten highly irradiated men, mostly reactor crew, from the Chernobyl accident is described. Their pre-accident medical conditions, and relevant medical status approximately 10-13y later are listed. A comparison is made between estimates, derived from several biological parameters, of their average whole-body penetrating radiation doses. First estimates were based on their presenting severity of prodromal sickness, early changes in blood cell counts, and dicentric chromosome aberrations in lymphocytes. In three cases ESR measurements on tooth enamel were also made. Retrospective dosimetry using FISH translocations was attempted 10-13 y later. This showed good agreement for those patients with the lower earlier dose estimates; up to about 3 Gy. For the others, extending up to about 12 Gy, the translocations indicated lower values, suggesting that in these cases translocations had somewhat declined. Repeated chromosomal examinations during the follow-up period showed an expected decline in dicentric frequencies. The pattern of decline was biphasic with a more rapid first phase, with a half-life of about 4 months followed by a slower decline with half-lives around 2-4 y. The rapid phase persisted for longer in those patients who had received the highest doses. 10-13 y later dicentric levels were still above normal background but well below the translocation frequencies.

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“…Since then, "Dicentric Chromosome Assay (DCA)" has been the preferred method for radiation dose assessment of occupationally or accidentally exposed humans. DCA was utilized for radiation dose assessment in the victims of several well-known radiation accidents such as Chernobyl [6,16,17], Goiania [18][19][20] and Fukushima-Daiichi [21][22][23], to name a few. DCA is considered to be the gold standard for absorbed dose assessment owing to its high specificity and sensitivity to IR.…”

Section: Fish Based Detection Of Dicentric Chromosomes For Absorbed Rmentioning

confidence: 99%

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Balajee¹

2018

obm genet

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The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3' end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genomes on various targets such as interphase nucleus, prematurely condensed chromosomes, metaphase chromosomes, fresh and paraffinized tissue sections. Although FISH is quite frequently used in clinical diagnostics, its use has recently been extended to the field of radiation biodosimetry where both stable (translocations) and unstable aberrations (dicentrics and rings) are detected with high resolution for estimating the absorbed radiation dose in humans after incidental, accidental or occupational exposure to ionizing radiation. This review summarizes the diverse applications of FISH in radiation biodosimetry that range from radiation dose estimation to prediction of deterministic (i.e. acute radiation syndrome severity) and stochastic (i.e. genetic mutations and cancer) effects in the affected human population.

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Novel data set for retrospective biodosimetry using both conventional and FISH chromosome analysis after high accidental overexposure

Cited by 14 publications

References 3 publications

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

A cytogenetic follow-up of some highly irradiated victims of the Chernobyl accident

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

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