Novel daily disposable therapeutic contact lenses based on chitosan and regenerated silk fibroin for the ophthalmic delivery of diclofenac sodium

Abstract

The aim of this study was to investigate the possibility of chitosan and regenerated silk fibroin (CS/ RSF) blended films as novel biomaterials for daily disposable therapeutic contact lenses based ophthalmic drug delivery system. Diclofenac sodium (DS), a hydrophilic anti-inflammatory agent, was loaded into CS/RSF films by a soaking method. The best conditions of DS loading manifested the loading time of 2 h and pH 6.5 of drug solution. The drug loading capacity and the drug release profile could be controlled by varying the film RSF content. With increasing the film RSF content from 0 to 30%, the amount of loaded DS increased from 12 to 23 mg. Furthermore, the prolong drug released within therapeutic level was obtained with increasing the film RSF content. Consequently, a fast released characteristic within a therapeutic level up to 3 h was observed with the 100CS/0RSF film. On the other hand, the 70CS/30RSF film demonstrated a significant prolonged drug release within therapeutic level up to 11 h. In conclusion, the CS/RSF films are promising as novel biomaterials for daily disposable therapeutic contact lenses-based ophthalmic delivery.

Chitin-Glucan Complex Hydrogels: Physical-Chemical Characterization, Stability, In Vitro Drug Permeation, and Biological Assessment in Primary Cells

“…In fact, the drug concentration of the loading solution has a direct effect on the hydrogels’ drug loading since the increase in the concentration of the drug leads to a higher amount of drug available to be loaded into the hydrogels’ structures. A similar effect has been reported for several drug delivery systems including calcium pectinate beads [ 46 ] and chitosan-silk fibroin films [ 47 ]. Indeed, the latter structures achieved an increase in diclofenac content from 1.075 to 4.564 µg/mm 3 using drug loading solutions of 62.5 and 250 µg/mL, respectively.…”

Kaempferol Incorporated Bovine Serum Albumin Fibrous Films for Ocular Drug Delivery

“…Water content is another important parameter for therapeutic contact lenses, a high water content would promote wearing comfort and oxygen permeability. [9,21] The embedded KAE caused little impact on the water content of the BSA films, all the BSA/KAE films possessed high water contents of >91% (Figure 1E), which surpassed most of the commercial contact lenses and displayed physicochemical properties just like hydrogels. The porous structure and the hydrophilicity of BSA assisted to retain water in the films.…”

“…In Vitro Drug Release: In vitro drug release was characterized according to a previous study. [9] A circular BSA/KAE film (diameter of 15 mm, thickness of ≈17 μm) was placed on a plastic filter head, with fluid cavity of 400 μL. Artificial tear fluid (compositions: 670 mg of NaCl, 200 mg of NaHCO 3 , 8 mg of CaCl 2 •2H 2 O, and deionized water added to 100 g) with a flow rate of 200 μL min −1 flowed through the film (Figure 2B), the filtrate was collected, and the amount of KAE released was determined by a Molecular Devices SpectraMax i3 at 368 nm.…”

“…[5,6] However, limited by the ocular surface protective barriers, the residence time of drugs on cornea is very DOI: 10.1002/mabi.202100269 short (2-5 min), thus the drug bioavailability of the eye drops is quite low (effective absorption < 5%). [7][8][9] Moreover, many drugs show poor solubility in aqueous solution, thus the as-prepared eye drops usually contain surfactants, which may aggravate the stimulation to the ocular wounds. In order to maintain drug efficacy, frequent administration of eye drops with high concentration of drugs are applied, which would not only reduce patient compliance, but also cause potential toxic side effects to the whole body.…”

“…[14] Chitosan/regenerated silk fibroin composite films load diclofenac sodium to form an ophthalmic drug delivery system, which shows tunable drug loading capacity and controllable drug release profile. [9] Considering the requirement of biocompatibility and hydrophilicity, therapeutic contact lenses fabricated by natural macromolecules are attracting continuous attention.…”