Novel cytoskeletal elements in mammalian eggs are composed of a unique arrangement of intermediate filaments

Gallicano, G. Ian; Larabell, Carolyn A.; McGaughey, Robert W.; Capco, David G.

doi:10.1016/0925-4773(94)90009-4

Cited by 49 publications

(37 citation statements)

References 43 publications

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“…[15][16][17][18] Regarding the molecular composition of the lattices, older studies suggested that the lattices were largely comprised of intermediate filaments, however more recent studies suggest that the lattices are also associated with microtubules. 19 In addition to their cytoskeletal composition, older and more recent studies have suggested that one function of the lattices is to store a pool of ribosomes that is needed for early cleavage divisions.…”

Section: Introductionmentioning

confidence: 99%

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

Liu

Morency

et al. 2017

Cell Cycle

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The oocyte cytoplasmic lattices (CPLs) have long been predicted to function as a storage form for the maternal contribution of ribosomes to the early embryo. Our previous studies have demonstrated that ribosomal component S6 is stored in the oocyte CPLs and peptidylarginine deiminase 6 (PADI6) is critical for CPLs formation. Additionally, we found that depletion of PADI6 reduced de novo protein synthesis prior to the maternal-to-embryonic transition, therefore causing embryos to arrest at the 2-cell stage. Here, we present evidence further supporting the association of ribosomes with the CPLs by demonstrating that rRNAs are dramatically decreased in Padi6 KO oocytes. We also show that the abundance and localization of mRNAs is affected upon PADI6 depletion, suggesting that mRNAs are very possibly associated with CPLs. Consistent with this observation, the amount of the major RNA binding protein, MSY2, that is associated with the insoluble fraction of the oocytes after Triton X-100 extraction is also markedly decreased in the Padi6 KO oocytes. Furthermore, treatment of the oocytes with RNase A followed by Triton X-100 extraction severely impairs the localization of PADI6 and MSY2 in oocytes. These results indicate that mRNAs, possibly in a complex with MSY2 and PADI6, are bound in the CPLs and may play a role in securing the mRNA-MSY2 complex to the CPLs.

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Section: Introductionmentioning

confidence: 99%

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

Liu

Morency

et al. 2017

Cell Cycle

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“…Conventional, resin-embedded transmission electron microscopy has been utilized by investigators as a powerful tool to interrogate the ultrastructural changes that accompany www.intechopen.com fertilization (Bement and Capco, 1989;Gallicano et al, 1991Gallicano et al, , 1994. However, mammalian eggs and embryos do not afford high contrast images in resin-embedded, ultrathin sections (silver to gold interference patterns corresponding to 60-70 nm).…”

Section: Conventional and Embedment-free Electron Microscopymentioning

confidence: 99%

“…ICB is composed of 100 mM KCl, 5 mM MgCl 2 , 3 mM EGTA, 20 mM HEPES, (pH 6.8). Tween-20 is the non-ionic detergent used for eggs and embryos as Triton X-100 was shown to remove selective peptides from and destabilize the cytoskeletal network in mammalian eggs and embryos (Gallicano et al, 1991(Gallicano et al, , 1994.…”

Section: Detergent Extractionmentioning

confidence: 99%

PKC Regulation of Gametogenesis and Early Development

Faust¹,

Kalive²,

Abraham³

et al. 2012

Meiosis - Molecular Mechanisms and Cytogenetic Diversity

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“…In some of these studies, vimentin and CKs were detected within the granulosa cells of follicles at various stages of growth and atresia, in luteal cells of the corpus luteum throughout the luteal phase, and in oocytes from both fetal and adult ovaries [Czernobilsky et al, 1985;Gall, 1991;Santini et al, 1993;Gallicano et al, 1994;van den Hurk et al, 1995;Marettová and Maretta, 2002]. Of vertebrate germ cells, nuclear IFs, called lamins, have thus far only been found in the oocytes of Xenopus [Gall et al, 1989] and in the oocytes of mice [Arnault et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

“…The expression of several classes of cytoplasmic IFs in the ovary has been described for several mammalian and avian species [Czernobilsky et al, 1985;Gall, 1991;Santini et al, 1993;Gallicano et al, 1994;Bukovsky et al, 1995;Ricken et al, 1995;van den Hurk et al, 1995;Pan and Auersperg, 1998;Löffler et al, 2000;Marettová and Maretta, 2002]. In some of these studies, vimentin and CKs were detected within the granulosa cells of follicles at various stages of growth and atresia, in luteal cells of the corpus luteum throughout the luteal phase, and in oocytes from both fetal and adult ovaries [Czernobilsky et al, 1985;Gall, 1991;Santini et al, 1993;Gallicano et al, 1994;van den Hurk et al, 1995;Marettová and Maretta, 2002].…”

Section: Introductionmentioning

confidence: 99%

Expression of Intermediate Filaments in the Balbiani Body and Ovarian Follicular Wall of the Japanese Quail <b><i>(Coturnix japonica)</i></b>

Rodler¹,

Sinowatz²

2013

Cells Tissues Organs

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In the present study, we examined the distribution of 6 groups of intermediate filaments (IFs; cytokeratins, CKs, vimentin, synemin, desmin, glial fibrillary acidic protein and lamins) in oocytes and follicular walls of the Japanese quail (Coturnix japonica) during their development using immunohistochemical and ultrastructural techniques. A distinctly vimentin- and synemin-positive Balbiani body, which is a transient accumulation of organelles (mitochondria, Golgi complex and endoplasmic reticulum) that occurs in the oocytes of all vertebrates including birds, could be detected in the oocytes of primordial and early pre-vitellogenic follicles. In larger pre-vitellogenic follicles, the Balbiani body has dispersed and the positivity of the granulosa cells appeared to concentrate in the basal portion of their cytoplasm. Our ultrastructural data demonstrated that the matrix of the Bal-biani body consists of fine IFs, which may play a role in the formation and dispersion of the Balbiani body. Of the CKs studied (panCK, CK5, CK7, CK8, CK14, CK15, CK18 and CK19), only CK5 showed a slight positive staining in both the theca externa and the Balbiani bodies of pre-vitellogenic oocytes. In conclusion, our data, which describe the changes in avian IF protein expression during folliculogenesis, suggest that the functions of the IFs (vimentin and synemin) of oocytes and follicular walls are not primarily mechanical but may be involved in the transient tethering of mitochondria in the area of the Balbiani body and in the gain of endocrine competence during the differentiation of granulosa cells.

show abstract

Novel cytoskeletal elements in mammalian eggs are composed of a unique arrangement of intermediate filaments

Cited by 49 publications

References 43 publications

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

PKC Regulation of Gametogenesis and Early Development

Expression of Intermediate Filaments in the Balbiani Body and Ovarian Follicular Wall of the Japanese Quail <b><i>(Coturnix japonica)</i></b>

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