Novel cytokine marker available for skin irritation testing of medical devices using reconstructed human epidermis models

Kato, Reiko; Miyajima, Atsuko; Komoriya, Kaoru; Haishima, Yuji

doi:10.1016/j.tiv.2020.104919

Cited by 2 publications

(2 citation statements)

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“…IL-1α is synthesized de novo in stimulated macrophages and can be actively secreted or passively released from apoptotic cells. IL-1α levels were studied as marker for irritation testing using reconstructed human epidermis models. , The assessment of potential toxicity and influence on IL-1α induction by synthetic mannolipids was determined using a reconstructed human epidermis model …”

Section: Resultsmentioning

confidence: 99%

“…IL-1α levels were studied as marker for irritation testing using reconstructed human epidermis models. 46,47 The assessment of potential toxicity and influence on IL-1α induction by synthetic mannolipids was determined using a reconstructed human epidermis model. 48 The production of another member of the IL-1 family (IL-1β) was also followed.…”

Section: Bioimmunological Characterization Of Polymers and Theirmentioning

confidence: 99%

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Cytotoxicity and Bioimmunological Activity of Poly(2-Isopropenyl-2-oxazoline) Conjugates with Ibuprofen Using 3D Reconstructed Tissue Models

Kroneková,

Majerčíková,

Paulovičová

et al. 2024

Biomacromolecules

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Poly(2-isopropenyl-2-oxazoline) (PIPOx) represents a universal polymer platform with pendant 2-oxazoline groups, allowing the preparation of biomaterials for various biomedical applications. However, there is a lack of information on PIPOx concerning the effect of molar mass (M n ) on cytotoxicity and bioimmunological properties. Here, aqueous copper(0)mediated reversible-deactivation radical polymerization (Cu 0 -RDPR) was used for the preparation of PIPOx with defined M n and low dispersity. PIPOx of different M n are used for the synthesis of conjugates with ibuprofen (5 mol %), the nonsteroidal antiinflammatory drug. The release of ibuprofen at 37 °C and different pH values is monitored using high-performance liquid chromatography, where the rate of drug release increases with increasing pH and lower M n . In vitro cytotoxicity and bioimmunological properties of PIPOx and drug conjugates are studied using 3D reconstructed tissue models of the human epidermis and intestinal epithelium. We demonstrate low cytotoxicity of PIPOx and conjugates with different M n values on both 3D tissue models.

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Section: Resultsmentioning

confidence: 99%