Novel conductive polypyrrole/zinc oxide/chitosan bionanocomposite: synthesis, characterization, antioxidant, and antibacterial activities

Abstract

An antibacterial and conductive bionanocomposite (BNC) film consisting of polypyrrole (Ppy), zinc oxide (ZnO) nanoparticles (NPs), and chitosan (CS) was electrochemically synthesized on indium tin oxide (ITO) glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO–Ppy/CS and ITO–Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%), while it was increased gradually for higher ratios (10, 15, and 20 wt%). The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa . The thermal stability and physical properties of BNC films were increased by an increase in the weight ratio of ZnO NPs, promising novel applications for the electrically conductive polysaccharide-based nanocomposites, particularly those that may exploit the antimicrobial nature of Ppy/ZnO/CS BNCs.

Robust self cleaning polypyrrole‐polysulfone blend hollow fiber membrane for biofouling mitigation

“…The observation suggests that 1 wt% concentration of PPy is sufficient to achieve absolute disinfection. The antibacterial activity of M3 membrane is mainly attributed to partially positively charged backbone of PPy particles disrupting the cell membranes of bacteria …”

“…9,10 Many researchers have reported the excellent antibacterial property of PPy against both Gram positive and Gram negative bacteria. [10][11][12] The bactericidal 3186 www.soci.org M Mukherjee, S De property of PPy is attributed to the charged backbone and not the leaching of biocidal materials, as reported by a number of studies. 11 Several nanocomposites and polymeric blends like PPy grafted silver, PPy-chitosan hybrid copolymer and PPy coated fabrics have shown striking antibacterial potential in the recent past.…”

“…Antibacterial activity of PPy has been studied over the last few years in combination with various metallic nanoparticles and other polymers . Many researchers have reported the excellent antibacterial property of PPy against both Gram positive and Gram negative bacteria . The bactericidal property of PPy is attributed to the charged backbone and not the leaching of biocidal materials, as reported by a number of studies .…”

“…11 Several nanocomposites and polymeric blends like PPy grafted silver, PPy-chitosan hybrid copolymer and PPy coated fabrics have shown striking antibacterial potential in the recent past. [10][11][12] However, there is scant literature investigating the blending of PPy into polymeric membrane and its biofouling and antibacterial potential during separation.…”

Tailoring Functional Chitosan‐Based Composites for Food Applications

“…Films based on chitosan with MWNT and Fe 3 O 4 , glutaraldehyde and aniline tetramer, graphene or polyaniline (alone or mixed with clay or silver nanoparticles – NP) showed typically low conductivity (between 0.00005–0.3 S/m) and modulus of elasticity (1.5–4.8 GPa) ,. However, the film with chitosan/polypyrrole/zinc oxide bionanocomposite had much higher conductivity (3355 S/m), but significant lower elasticity (100 times less) …”